Molecular Identification of Strains of Caridean Species in a Tropical Lagoons of South-Western Nigeria

Species identification by morphological approach requires a high degree of experience, which is difficult and not practical enough for those interested in surveying a broad diversity of organisms. However, molecular or genetic approaches to identify species have been proposed and widely used by most scientists. DNA barcoding was used to identify Macrobrachium species occurring in three coastal lagoons (Badagry, Lagos and Epe) in South-western Nigeria. Samples of prawns for the DNA studies were collected from May to July 2015. The DNA of the Macrobrachium species was extracted using the phenol-chloroform protocol and Norgen tissue kit. Amplification and sequencing of the Macrobrachium species were carried out and further identification was done by comparing with sequenced data in the Genbank. New species of Macrobrachium were obtained in Badagry and Epe Lagoons having a close match with M. asperulum and M. nipponense at 85% and 84% respectively. M. asperulum and M. nipponense are reported in Badagry and Epe Lagoons for the first time.

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Benchmarking DNA barcodes: an assessment using available primate sequences

Genome ◽

10.1139/g06-025 ◽

2006 ◽

Vol 49 (7) ◽

pp. 851-854 ◽

Cited By ~ 48

Author(s):

Mehrdad Hajibabaei ◽

Gregory AC Singer ◽

Donal A Hickey

Keyword(s):

New Species ◽

Cryptic Species ◽

Dna Barcoding ◽

Species Identification ◽

Dna Sequences ◽

Phylogenetic Relationships ◽

Primate Species ◽

Dna Barcodes ◽

Global Biodiversity ◽

Efficient Sequence

DNA barcoding has been recently promoted as a method for both assigning specimens to known species and for discovering new and cryptic species. Here we test both the potential and the limitations of DNA barcodes by analysing a group of well-studied organisms—the primates. Our results show that DNA barcodes provide enough information to efficiently identify and delineate primate species, but that they cannot reliably uncover many of the deeper phylogenetic relationships. Our conclusion is that these short DNA sequences do not contain enough information to build reliable molecular phylogenies or define new species, but that they can provide efficient sequence tags for assigning unknown specimens to known species. As such, DNA barcoding provides enormous potential for use in global biodiversity studies.Key words: DNA barcoding, species identification, primate, biodiversity.

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Description of new apterous winter species of Leuctra (Plecoptera: Leuctridae) based morphology and DNA barcoding and further records to stonefly fauna of the Caucasus, Georgia

Zootaxa ◽

10.11646/zootaxa.4585.3.9 ◽

2019 ◽

Vol 4585 (3) ◽

pp. 547

Author(s):

VALENTINA A. TESLENKO ◽

DMITRY M. PALATOV ◽

ALEXANDER A. SEMENCHENKO

Keyword(s):

New Species ◽

Dna Barcoding ◽

Interspecific Variation ◽

Genetic Distances ◽

Dna Barcodes ◽

The Caucasus ◽

Diagnostic Characters ◽

Males And Females ◽

Lesser Caucasus ◽

First Time

Leuctra adjariae sp. n. and Leuctra georgiae sp. n. (Plecoptera: Leuctridae) are described as two new apterous stonefly species from the Meskheti Range (Lesser Caucasus) in southwestern Georgia. Descriptions and illustrations are provided for both sexes and diagnostic characters are discussed. Males and females of the two species are associated by DNA barcodes. Comparisons with corresponding regions of COI between L. adjariae sp. n. and L. georgiae sp. n. produced K2P genetic distances of 8.38%, values well associated with interspecific variation. The well-supported monophyly as well as results of an ABGD analysis confirms the validity of both new species. Capnioneura gouanerae Vinçon & Sivec, 2011, previously described and known only from Turkey, is reported for the first time for the Caucasus.

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A first phylogenetic appraisal of two allied genera of Afrotropical Ceratocanthinae: Melanophilharmostes and Pseudopterorthochaetes (Coleoptera: Hybosoridae)

Fragmenta Entomologica ◽

10.4081/fe.2016.159 ◽

2016 ◽

Vol 48 (1) ◽

pp. 33 ◽

Cited By ~ 1

Author(s):

Alberto Ballerio

Keyword(s):

New Species ◽

Burkina Faso ◽

Miombo Woodlands ◽

Morphological Approach ◽

Three New Species ◽

First Time ◽

Ecological Range

Two genera of Ceratocanthinae (Coleoptera Scarabaeoidea Hybosoridae), Melanophilharmostes Paulian, 1968 and Pseudopterorthochaetes Paulian, 1977, are evaluated through a cladistic morphological approach, resulting in the transfer of Melanophilharmostes demirei Paulian, 1977 to Pseudopterorthochaetes. All species are catalogued and all except two are keyed. Three new species are described: Melanophilharmostes poggii n. sp. from Annobon island (Ecuatorial Guinea), Pseudopterorthochaetes genierorum n. sp. and P. miomboincola n. sp., both from Mozambique. Melanophilharmostes zicsii (Paulian, 1968) is recorded for the first time for Burkina Faso, marking the northernmost record for African Ceratocanthinae. The records in Sudanian savannas and Miombo woodlands extend the ecological range of the subfamily.

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Taxonomy of the genus Craspedomerus Bernhauer, 1911 (Coleoptera: Staphylinidae: Philonthina) from China

Zootaxa ◽

10.11646/zootaxa.2506.1.1 ◽

2010 ◽

Vol 2506 (1) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

LIANG LI ◽

HONG-ZHANG ZHOU

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

New Species ◽

Species Identification ◽

Geographical Distribution ◽

Additional Species ◽

Three New Species ◽

First Time ◽

Scanning Electron

Three new species of the genus Craspedomerus from China are described and illustrated: C. giganteus Li & Zhou sp. n. from Sichuan, C. gongshanus Li & Zhou sp. n. from Yunnan and C. zhangi Li & Zhou sp. n. from Tibet. Four species are reported for the first time from China: C. sinetuber (Coiffait, 1977a) from Tibet, C. cyanipennis Scheerpeltz, 1976b, C. ganeshensis Coiffait, 1983 and C. glenoides (Schubert, 1908) from Yunnan. These four and two additional species previously recorded from China (C. beckeri Bernhauer, 1934 and C. violaceipennis Cameron, 1928 are redescribed and illustrated. Sensory peg setae located on the underside of the paramere of the aedeagus of all nine Chinese species are compared using scanning electron microscopy (SEM) and the result shows that this character is useful for species identification. A key to the Chinese species of Craspedomerus is presented and geographical distribution of all sixteen species of Craspedomerus is mapped.

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Newly Emerging Pest in China, Rhynchaenus maculosus (Coleoptera: Curculionidae): Morphology and Molecular Identification with DNA Barcoding

Insects ◽

10.3390/insects12060568 ◽

2021 ◽

Vol 12 (6) ◽

pp. 568

Author(s):

Rui-Sheng Yang ◽

Ming-Yang Ni ◽

Yu-Jian Gu ◽

Jia-Sheng Xu ◽

Ying Jin ◽

...

Keyword(s):

Dna Barcoding ◽

Species Identification ◽

Molecular Identification ◽

Morphological Characters ◽

Information Storage ◽

Morphological Description ◽

Quick Response ◽

Dna Barcodes ◽

Pairwise Divergence ◽

Coding Capacity

The oak flea weevil, Rhynchaenus maculosus Yang et Zhang 1991, is a newly emerging pest that severely damages oak (genus Quercus) in China. The first R. maculosus outbreak occurred in 2020 and caused spectacular damage to all oak forests in Jilin province, northeast China. The lack of key morphological characters complicates the identification of this native pest, especially in larva and pupa stages. This is problematic because quick and accurate species identification is crucial for early monitoring and intervention during outbreaks. Here, we provided the first detailed morphological description of R. maculosus at four life stages. Additionally, we used DNA barcodes from larva and pupa specimens collected from three remote locations for molecular identification. The average pairwise divergence of all sequences in this study was 0.51%, well below the 2% to 3% (K-2-parameter) threshold set for one species. All sample sequences matched the R. maculosus morphospecies (KX657706.1 and KX657707.1), with 99.23% to 100% (sequence identity, E value: 0.00) matching success. The tree based on barcodes placed the specimens into the Rhynchaenus group, and the phylogenetic relationship between 62 sequences (30 samples and 32 from GeneBank) had high congruence with the morphospecies taxa. The traditional DNA barcodes were successfully transformed into quick response codes with larger coding capacity for information storage. The results showed that DNA barcoding is reliable for R. maculosus identification. The integration of molecular and morphology-based methods contributes to accurate species identification of this newly emerging oak pest.

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Discovery of the genus Claraeola Aczél in Iran with the description of two new species (Diptera: Pipunculidae)

Zootaxa ◽

10.11646/zootaxa.4227.4.6 ◽

2017 ◽

Vol 4227 (4) ◽

pp. 563 ◽

Cited By ~ 2

Author(s):

BEHNAM MOTAMEDINIA ◽

CHRISTIAN KEHLMAIER ◽

AZIZOLLAH MOKHTARI ◽

EHSAN RAKHSHANI ◽

EBRAHIM GILASIAN

Keyword(s):

New Species ◽

Dna Barcoding ◽

Identification Key ◽

Coi Gene ◽

Palaearctic Species ◽

Mitochondrial Coi ◽

Mitochondrial Coi Gene ◽

Two New Species ◽

Western Palaearctic ◽

First Time

The genus Claraeola Aczél is recorded from Iran for the first time. Two new species, Claraeola parnianae Motamedinia & Kehlmaier sp. nov. and Claraeola khorshidae Motamedinia & Kehlmaier sp. nov., are described and illustrated. An updated identification key to the Western Palaearctic species of the genus Claraeola is provided. Both species were characterized morphologically and by DNA barcoding of the mitochondrial COI gene.

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Taxonomic revision of the spider genus Ixchela Huber, 2000 (Araneae: Pholcidae), with description of ten new species from Mexico and Central America

Zootaxa ◽

10.11646/zootaxa.3608.5.1 ◽

2013 ◽

Vol 3608 (5) ◽

pp. 285-327 ◽

Cited By ~ 2

Author(s):

ALEJANDRO VALDEZ-MONDRAGÓN

Keyword(s):

New Species ◽

Central America ◽

Species Identification ◽

Taxonomic Revision ◽

Identification Keys ◽

Taxonomic Information ◽

Males And Females ◽

First Time ◽

New Distribution

A taxonomic revision of the genus Ixchela Huber, 2000 is presented. The previously described five species are redescribed with new taxonomic information and new distribution records. The males of Ixchela placida (Gertsch, 1971) and Ixchela simoni (O. Pickard-Cambridge, 1898) are described for the first time. Ten new species are described, nine from Mexico and one from Honduras: Ixchela mixe new species, Ixchela huberi new species, Ixchela juarezi new species, Ixchela grix new species, Ixchela taxco new species, Ixchela franckei new species, Ixchela tzotzil new species, Ixchela santibanezi new species, Ixchela huasteca new species, and Ixchela viquezi new species. Identification keys for males and females are presented.

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Three new species of Coecobrya (Collembola: Entomobryidae) from caves in the Thai Peninsula

Zootaxa ◽

10.11646/zootaxa.4286.2.3 ◽

2017 ◽

Vol 4286 (2) ◽

pp. 187 ◽

Cited By ~ 3

Author(s):

AREERUK NILSAI ◽

SOPARK JANTARIT ◽

CHUTAMAS SATASOOK ◽

FENG ZHANG

Keyword(s):

New Species ◽

Genetic Distance ◽

Dna Barcoding ◽

Labial Palp ◽

Outer Lobe ◽

Coi Sequences ◽

Morphological Differences ◽

Three New Species ◽

Great Genetic Distance ◽

First Time

Three new species of the genus Coecobrya are described from caves in the Thai Peninsula for the first time: C. cavicta sp. nov. and C. polychaeta sp. nov. from Satun Province, and C. chumphonensis sp. nov. from Chumphon Province. These species differ in antennal length, clypeal chaetae, labial palp, sublobal chaetae of maxillary outer lobe, labial and postlabial chaetae, claw, ventral tube, manubrial plaque, and dorsal cephalic and tergal chaetotaxy. They are similar to C. annulata Zhang, Bedos & Deharveng, 2016 but differ from the latter in the combination of antennal length, claw and dorsal chaetotaxy. DNA barcoding COI sequences are provided for two species and three populations and key to the Thai species is also given. One population, which exhibits a great genetic distance (0.219–0.239) and minor but stable morphological differences from C. polychaeta sp. nov., cannot be accepted as a full species as the evidence is insufficient.

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Contributions to the millipede genus Nepalella Shear, 1979 from China, with four new species and first results on phylogeny based on DNA-barcoding (Diplopoda, Chordeumatida, Megalotylidae)

Zootaxa ◽

10.11646/zootaxa.4243.3.3 ◽

2017 ◽

Vol 4243 (3) ◽

pp. 455 ◽

Cited By ~ 3

Author(s):

WEIXIN LIU ◽

THOMAS WESENER ◽

SERGEI GOLOVATCH ◽

MINGYI TIAN

Keyword(s):

New Species ◽

Genetic Distance ◽

Dna Barcoding ◽

Southern China ◽

Mitochondrial Gene ◽

First Results ◽

First Time

Four new species of the chordeumatidan millipede genus Nepalella Shear, 1979, family Megalotylidae, are described from caves in southern China: N. troglodytes sp. nov., N. lobata sp. nov., N. jinfoshan sp. nov., and N. wangi sp. nov. Three of them (except N. lobata sp. nov.) are presumed troglobites. Additional locality records of two cave congeners, N. caeca Shear, 1999 and N. grandoides Golovatch, Geoffroy & Mauriès, 2006, are also provided. DNA-barcoding based on the COI mitochondrial gene is documented in this genus and for species of the order Chordeumatida outside Europe for the first time. Interspecific p-distances between Nepalella species amount to 8.5–15.9%, while intraspecific p-distances are 0–6.8%. The species of Nepalella are found to show a surprisingly low genetic distance from the European genus Atractosoma Fanzago, 1876, family Craspedosomatidae Gray in Jones, 1843, potentially based on the very limited number of barcoding sequences of the order Chordeumatida being available.

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The tribe Anthocorini in Japan (Hemiptera: Anthocoridae): descriptions of new species, review of distribution and bionomics

Acta Entomologica Musei Nationalis Pragae ◽

10.37520/aemnp.2021.022 ◽

2021 ◽

pp. 375-426

Author(s):

Kazutaka Yamada ◽

Tomohide Yasunaga

Keyword(s):

New Species ◽

Species Identification ◽

Japanese Species ◽

Widespread Species ◽

Male And Female ◽

Distribution Maps ◽

Two New Species ◽

Anthocoris Nemoralis ◽

First Time ◽

Locality Information

Japanese Anthocorini are reviewed. A total of five genera and 13 species were identified, including two new species described below: Temnostethus mirificus sp. nov. and Anthocoris venustus sp. nov. Previous records of Anthocoris miyamotoi Hiura, 1959 from Japan are considered to be confused with A. venustus sp. nov. Anthocoris nemoralis (Fabricius, 1794), which is a common and widespread species in Europe, is recorded in Japan for the first time; it is assumed to have been accidentally introduced into Japan recently. Temnostethus distans Kerzhner, 1973 and Anthocoris kalopanacis Kerzhner, 1977 are newly recorded in Japan proper. Male and female genitalia are illustrated and described for most of the treated species. Identification keys to the genera and species that occur in Japan are provided. Bionomics are provided for each species. Detailed locality information and distribution maps in Japan are presented for all Japanese species and their zoogeography is discussed.

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