Apoptosis inducing effects of chlorhexidine and essential oil mouthwashes on BHK-21 fibroblast cell line: An in vitro study

Background: The maintenance of oral health can be achieved mainly by mechanical and chemical means. Among chemical agents, mouthwashes are widely used for personal oral hygiene because of their ability to inhibit dental plaque. The antibacterial effects of essential oils (EOs) and chlorhexidine (CHX) are well documented; however, the reaction of host tissue to these substances has a poor documentation. Until now studies have not examined the effect of EOs with sodium fluoride (EOF) on fibroblast cell lines. The aim of this study was to examine the effect of mouth rinse EOs, EOF and CHX on the apoptosis of fibroblast cell line. Methods: BHK-21 fibroblast cell line was cultured and incubated in Eagle's Minimum Essential Medium containing EOs, EOF and CHX mouthwashes with different doses (15% or 25%) and various exposure times. Cell apoptosis was assayed using RT-PCR. Results: EOs, EOF and CHX induce apoptotic effects on fibroblasts in a dose and time dependent manner. Conclusion: CHX is the most cytotoxic mouthwash to fibroblasts as compared to mouthwashes containing EOs and EOF.

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Comparative Evaluation of Cytotoxicity and Genotoxicity of Two Bioceramic Sealers on Fibroblast Cell Line: An in vitro Study

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-2315 ◽

2018 ◽

Vol 19 (6) ◽

pp. 656-661 ◽

Cited By ~ 4

Author(s):

Moksha Nayak ◽

CN Vijay Kumar ◽

Veena Shetty ◽

Anoop V Nair ◽

L Krishna Prasada ◽

...

Keyword(s):

Cell Line ◽

Comparative Evaluation ◽

In Vitro Study ◽

Fibroblast Cell ◽

Vitro Study ◽

Fibroblast Cell Line

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Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study

Journal of Conservative Dentistry ◽

10.4103/jcd.jcd_518_18 ◽

2019 ◽

Vol 22 (2) ◽

pp. 160 ◽

Cited By ~ 2

Author(s):

KiranR Halkai ◽

JayashreeA Mudda ◽

Vasundhara Shivanna ◽

Veena Patil ◽

Vandana Rathod ◽

...

Keyword(s):

Silver Nanoparticles ◽

Cell Line ◽

In Vitro Study ◽

Fibroblast Cell ◽

Vitro Study ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Fibroblast Cell Line

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In vitro study for laser gene transfer in BHK-21 fibroblast cell line

10.1117/12.802507 ◽

2009 ◽

Author(s):

M. Abdel Aziz ◽

D. S. Salem ◽

M. S. Salama ◽

Y. Badr

Keyword(s):

Gene Transfer ◽

Cell Line ◽

In Vitro Study ◽

Fibroblast Cell ◽

Vitro Study ◽

Fibroblast Cell Line

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In vitro evaluation of cytomechanics of osteoblast and fibroblast cell line for enhanced peri-implant bone and soft tissue engineering

Clinical Oral Implants Research ◽

10.1111/clr.18_13356 ◽

2018 ◽

Vol 29 ◽

pp. 61-61

Author(s):

Santosh Y. Nelogi ◽

Maheshwari Nelogi

Keyword(s):

Tissue Engineering ◽

Soft Tissue ◽

Cell Line ◽

Fibroblast Cell ◽

Fibroblast Cell Line ◽

In Vitro Evaluation ◽

Soft Tissue Engineering

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In vitro and in silico modeling of perfluoroalkyl substances mixture toxicity in an amphibian fibroblast cell line

Chemosphere ◽

10.1016/j.chemosphere.2019.05.065 ◽

2019 ◽

Vol 233 ◽

pp. 25-33 ◽

Cited By ~ 16

Author(s):

Gary Hoover ◽

Supratik Kar ◽

Samuel Guffey ◽

Jerzy Leszczynski ◽

Maria S. Sepúlveda

Keyword(s):

Cell Line ◽

In Silico ◽

Mixture Toxicity ◽

Fibroblast Cell ◽

Perfluoroalkyl Substances ◽

Fibroblast Cell Line ◽

In Silico Modeling

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Modeling atrial fibrosis in vitro —Generation and characterization of a novel human atrial fibroblast cell line

FEBS Open Bio ◽

10.1002/2211-5463.12896 ◽

2020 ◽

Vol 10 (7) ◽

pp. 1210-1218 ◽

Cited By ~ 2

Author(s):

Stephan R. Künzel ◽

Johanna S. E. Rausch ◽

Charlotte Schäffer ◽

Maximilian Hoffmann ◽

Karolina Künzel ◽

...

Keyword(s):

Cell Line ◽

Fibroblast Cell ◽

Fibroblast Cell Line ◽

Atrial Fibrosis

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Fibroblast cell line establishment, cryopreservation and interspecies embryos reconstruction in red panda (Ailurus fulgens)

Zygote ◽

10.1017/s0967199408004966 ◽

2009 ◽

Vol 17 (2) ◽

pp. 117-124 ◽

Cited By ~ 10

Author(s):

Yong Tao ◽

Jianming Liu ◽

Yunhai Zhang ◽

Meiling Zhang ◽

Junshun Fang ◽

...

Keyword(s):

Somatic Cell ◽

Cell Line ◽

Fibroblast Cell ◽

Basic Medium ◽

Fibroblast Cell Line ◽

Cell Line Establishment ◽

Red Panda ◽

Fetal Bovine ◽

Ailurus Fulgens

SummaryIn evolution, the red panda (Ailurus fulgens) plays a pivotal role in the higher level phylogeny of arctoides carnivore mammals. The red panda inhabits certain Asian countries only and its numbers are decreasing. Therefore, the development of feasible ways to preserve this species is necessary. Genetic resource cryopreservation and somatic cell nuclear transfer (SCNT) have been used extensively to rescue this endangered species. The present study describes the establishment, for the first time, of a red panda ear fibroblast cell line, which was then cryopreserved, thawed and cultured. Through micromanipulation, interspecies embryos were reconstructed using the cryopreserved–thawed fibroblasts of the red panda as the donor and rabbit oocytes as recipients. A total of 194 enucleated rabbit oocytes were reconstructed with red panda ear fibroblasts; enucleated oocytes were activated without fusion as the control. The results show that the fibroblast cell line was established successfully by tissue culture and then cryopreserved in liquid nitrogen. Supplementation with 20% fetal bovine serum and 8% dimethyl sulphoxide in basic medium facilitated the cryopreservation. The interspecies embryos were successfully reconstructed. The cleavage, morulae and blastocyst rates after in vitro culture were 71, 47 and 23% (31/194), respectively. This study indicated that a somatic cell line could be established and cryopreserved from red panda and that rabbit cytoplast supports mitotic cleavage of the red panda karyoplasts and is capable of reprogramming the nucleus to achieve blastocysts.

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