Classification of the nucleolytic ribozymes based upon catalytic mechanism
Keyword(s):
The nucleolytic ribozymes carry out site-specific RNA cleavage reactions by nucleophilic attack of the 2′-oxygen atom on the adjacent phosphorus with an acceleration of a million-fold or greater. A major part of this arises from concerted general acid–base catalysis. Recent identification of new ribozymes has expanded the group to a total of nine and this provides a new opportunity to identify sub-groupings according to the nature of the general base and acid. These include nucleobases, hydrated metal ions, and 2′-hydroxyl groups. Evolution has selected a number of different combinations of these elements that lead to efficient catalysis. These differences provide a new mechanistic basis for classifying these ribozymes.
1992 ◽
Vol 114
(14)
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pp. 5643-5646
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2011 ◽
Vol 366
(1580)
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pp. 2910-2917
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1984 ◽
pp. 745-751
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1978 ◽
pp. 663-667
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2000 ◽
Vol 122
(9)
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pp. 2062-2068
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1976 ◽
Vol 98
(4)
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pp. 1057-1059
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2021 ◽
2014 ◽
Vol 70
(9)
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pp. 2256-2263
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