scholarly journals Induction of Somatic Embryos and Plantlet Development in Cell Suspension Cultures of Arachis hypogaea L.

1998 ◽  
Vol 48 (3) ◽  
pp. 231-236
Author(s):  
Perumal Venkatachalam ◽  
Natesan Geetha ◽  
Narayanasamipillai Jayabalan
Keyword(s):  
Cell Suspension ◽  
Arachis Hypogaea ◽  
Somatic Embryos ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Arachis Hypogaea L

ABA enhances plant regeneration of somatic embryos derived from cell suspension cultures of plantain cv. Spambia (Musa sp.)

2009 ◽  
Vol 99 (2) ◽  
pp. 133-140 ◽  
Author(s):  
Nasser J. Y. Sholi ◽  
Anjana Chaurasia ◽  
Anuradha Agrawal ◽  
Neera Bhalla Sarin
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
Somatic Embryos ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Musa Sp

scholarly journals Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hyun-A Woo ◽  
Seong Sub Ku ◽  
Eun Yee Jie ◽  
HyeRan Kim ◽  
Hyun-Soon Kim ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Regeneration System ◽  
Embryogenic Cell ◽  
Embryogenic Callus Formation

AbstractTo establish an efficient plant regeneration system from cell suspension cultures of Euonymus alatus, embryogenic callus formation from immature embryos was investigated. The highest frequency of embryogenic callus formation reached 50% when the immature zygotic embryos were incubated on Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D). At higher concentrations of 2,4-D (over 2 mg/L), the frequency of embryogenic callus formation declined significantly. The total number of somatic embryos development was highest with the 3% (w/v) sucrose treatment, which was found to be the optimal concentration for somatic embryo formation. Activated charcoal (AC) and 6-benzyladenine (BA) significantly increased the frequency of plantlet conversion from somatic embryos, but gibberellic acid (GA3) had a negative effect on plantlet conversion and subsequent development from somatic embryos. Even though the cell suspension cultures were maintained for more than 1 year, cell aggregates from embryogenic cell suspension cultures were successfully converted into normal somatic embryos with two cotyledons. To our knowledge, this is the first successful report of a plant regeneration system of E. alatus via somatic embryogenesis. Thus, the embryogenic cell line and plant regeneration system established in this study can be applied to mass proliferation and production of pharmaceutical metabolite in E. alatus.

Development of somatic embryos from cell suspension cultures of Norway spruce (Picea abies Karst.)

1988 ◽  
Vol 7 (2) ◽  
pp. 134-137 ◽  
Author(s):  
M. P. Boulay ◽  
P. K. Gupta ◽  
P. Krogstrup ◽  
D. J. Durzan
Keyword(s):  
Picea Abies ◽  
Cell Suspension ◽  
Norway Spruce ◽  
Somatic Embryos ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

scholarly journals Somatic embryogenesis and plant regeneration from cell suspension cultures of Gentiana kurroo Royle.

2018 ◽  
Vol 7 (5) ◽  
pp. 2239
Author(s):  
Shiwani Kaushal ◽  
Arushdeep Sidana
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Cell Suspension ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Western Himalayas ◽  
Ms Medium ◽  
Gentiana Kurroo

Gentiana kurroo Royle, native to North- Western Himalayas, is one of the critically endangered perennial herbs, which is being overexploited due to its multiple medicinal uses. The goal of this work was to develop a protocol for somatic embryogenesis and efficient plant regeneration through cell suspension culture. Selected ranges of plant growth regulators were experimented for somatic embryogenesis. Light micrographs of the established suspension cell cultures of somatic embryos at different developmental stages were also observed under light microscope. Somatic embryogenesis was induced from the leaf explants and the maximum induction frequency (97.2%) was obtained on Murashige and Skoog (MS) medium supplemented with 0.5 mg/l each of NAA, BAP and TDZ. Cell suspension cultures were established in MS liquid medium containing IAA (0.5 mg/l) + BAP (1.0 mg/l) with 89-93% viable cells. Transfer of cotyledonary somatic embryos to the MS agar medium containing 2, 4-D (0.4 mg/l) and KN (1.5 mg/l) resulted in somatic embryogenesis at high frequencies (80%) with an average of 28 ± 0.2 embryos/ callus piece. After transfer onto the half strength MS medium without growth regulators approximately 80-90% somatic embryos developed into complete plantlets. The protocol described here could be used for somatic hybridization, genetic transformation, isolation of protoplasts and large-scale propagation of G. kurroo.

Transformation of Papaver somniferum cell suspension cultures with sam1 from A. thaliana results in cell lines of different S-adenosyl-L-methionine synthetase activity

1997 ◽  
Vol 99 (2) ◽  
pp. 233-240 ◽  
Author(s):  
Muriel Belny ◽  
Didier Herouart ◽  
Brigitte Thomasset ◽  
Helene David ◽  
Annie Jacquin-Dubreuil ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Cell Lines ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Papaver Somniferum ◽  
Synthetase Activity
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