ABA enhances plant regeneration of somatic embryos derived from cell suspension cultures of plantain cv. Spambia (Musa sp.)

AbstractTo establish an efficient plant regeneration system from cell suspension cultures of Euonymus alatus, embryogenic callus formation from immature embryos was investigated. The highest frequency of embryogenic callus formation reached 50% when the immature zygotic embryos were incubated on Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D). At higher concentrations of 2,4-D (over 2 mg/L), the frequency of embryogenic callus formation declined significantly. The total number of somatic embryos development was highest with the 3% (w/v) sucrose treatment, which was found to be the optimal concentration for somatic embryo formation. Activated charcoal (AC) and 6-benzyladenine (BA) significantly increased the frequency of plantlet conversion from somatic embryos, but gibberellic acid (GA3) had a negative effect on plantlet conversion and subsequent development from somatic embryos. Even though the cell suspension cultures were maintained for more than 1 year, cell aggregates from embryogenic cell suspension cultures were successfully converted into normal somatic embryos with two cotyledons. To our knowledge, this is the first successful report of a plant regeneration system of E. alatus via somatic embryogenesis. Thus, the embryogenic cell line and plant regeneration system established in this study can be applied to mass proliferation and production of pharmaceutical metabolite in E. alatus.

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Somatic embryogenesis and plant regeneration from cell suspension cultures of Gentiana kurroo Royle.

Annals of Plant Sciences ◽

10.21746/aps.2018.7.5.6 ◽

2018 ◽

Vol 7 (5) ◽

pp. 2239

Author(s):

Shiwani Kaushal ◽

Arushdeep Sidana

Keyword(s):

Somatic Embryogenesis ◽

Plant Regeneration ◽

Cell Suspension ◽

Growth Regulators ◽

Somatic Embryos ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Western Himalayas ◽

Ms Medium ◽

Gentiana Kurroo

Gentiana kurroo Royle, native to North- Western Himalayas, is one of the critically endangered perennial herbs, which is being overexploited due to its multiple medicinal uses. The goal of this work was to develop a protocol for somatic embryogenesis and efficient plant regeneration through cell suspension culture. Selected ranges of plant growth regulators were experimented for somatic embryogenesis. Light micrographs of the established suspension cell cultures of somatic embryos at different developmental stages were also observed under light microscope. Somatic embryogenesis was induced from the leaf explants and the maximum induction frequency (97.2%) was obtained on Murashige and Skoog (MS) medium supplemented with 0.5 mg/l each of NAA, BAP and TDZ. Cell suspension cultures were established in MS liquid medium containing IAA (0.5 mg/l) + BAP (1.0 mg/l) with 89-93% viable cells. Transfer of cotyledonary somatic embryos to the MS agar medium containing 2, 4-D (0.4 mg/l) and KN (1.5 mg/l) resulted in somatic embryogenesis at high frequencies (80%) with an average of 28 ± 0.2 embryos/ callus piece. After transfer onto the half strength MS medium without growth regulators approximately 80-90% somatic embryos developed into complete plantlets. The protocol described here could be used for somatic hybridization, genetic transformation, isolation of protoplasts and large-scale propagation of G. kurroo.

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High frequency plant regeneration via somatic embryogenesis in cell suspension cultures of cowpea, Vigna unguiculata (L.) Walp.

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-000-0085-4 ◽

2000 ◽

Vol 36 (6) ◽

pp. 475-480 ◽

Cited By ~ 16

Author(s):

R. Prem Anand ◽

A. Ganapathi ◽

V. Ramesh Anbazhagan ◽

G. Vengadesan ◽

N. Selvaraj

Keyword(s):

Somatic Embryogenesis ◽

Plant Regeneration ◽

Cell Suspension ◽

Vigna Unguiculata ◽

High Frequency ◽

Cell Suspension Cultures ◽

Suspension Cultures

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Characterization and plant regeneration of cell suspension cultures of Lycopersicon chilense

Canadian Journal of Botany ◽

10.1139/b91-283 ◽

1991 ◽

Vol 69 (10) ◽

pp. 2257-2260 ◽

Cited By ~ 4

Author(s):

Ann Francine Greer ◽

Zohreh Tabaeizadeh

Keyword(s):

Plant Regeneration ◽

Cell Suspension ◽

Casein Hydrolysate ◽

Leaf Explants ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Cell Suspensions ◽

Lag Phase ◽

Petiole Explants ◽

Lycopersicon Chilense

To produce calli for the establishment of a cell suspension, leaf, stem, and petiole explants of Lycopersicon chilense Dun., grown in vitro and in the soil, were cultured on media containing 15 different combinations of benzylaminopurine, kinetin, and indole acetic acid. Among the three types of tissues, leaf explants showed the best response. Cell suspension cultures of L. chilense were established from leaf callus derived from soil grown plants using Murashige and Skoog's medium supplemented with casein hydrolysate (250 mg/L), coconut water (5%), and 2,4-dichlorophenoxyacetic acid (2 mg/L). Once established, cell suspensions showed a rapid growth rate with no marked lag phase. Shooting via organogenesis occurred from callus derived from cell suspensions on medium containing 2 mg/L benzylaminopurine. Regenerated plants had the same morphology as the original plants. Key words: Lycopersicon chilense, tomato, tissue culture, cell suspensions, organogenesis, plant regeneration.

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