scholarly journals Cloning and amplification of a gene for glutathione synthetase in Escherichia coli B.

1983 ◽  
Vol 47 (6) ◽  
pp. 1381-1383 ◽  
Author(s):  
Kousaku MURATA ◽  
Toyofumi MIYA ◽  
Hiroshi GUSHIMA ◽  
Akira KIMURA
Keyword(s):  
Escherichia Coli ◽  
Glutathione Synthetase ◽  
Escherichia Coli B

Use of adenosine(5')polyphospho(5')pyridoxals to study the substrate-binding region of glutathione synthetase from Escherichia coli B

Biochemistry ◽  
1993 ◽  
Vol 32 (6) ◽  
pp. 1548-1554 ◽  
Author(s):  
Takao Hibi ◽  
Hiroaki Kato ◽  
Takaaki Nishioka ◽  
Junichi Oda ◽  
Hiroshi Yamaguchi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Substrate Binding ◽  
Glutathione Synthetase ◽  
Binding Region ◽  
Escherichia Coli B

Three-dimensional Structure of the Glutathione Synthetase from Escherichia coli B at 2·0 Å Resolution

1993 ◽  
Vol 229 (4) ◽  
pp. 1083-1100 ◽  
Author(s):  
Hiroshi Yamaguchi ◽  
Hiroaki Kato ◽  
Yasuo Hata ◽  
Takaaki Nishioka ◽  
Akira Kimura ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Glutathione Synthetase ◽  
Three Dimensional Structure ◽  
Escherichia Coli B

Expression of the glutathione synthetase gene of Escherichia coli B in Saccharomyces cerevisiae

1989 ◽  
Vol 68 (6) ◽  
pp. 390-394 ◽  
Author(s):  
Yasuyuki Ohtake ◽  
Kunihiko Watanabe ◽  
Hidetoshi Tezuka ◽  
Tomoo Ogata ◽  
Seizou Yabuuchi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Glutathione Synthetase ◽  
Escherichia Coli B

Crystallization and preliminary X-ray studies of glutathione synthetase from Escherichia coli B

1989 ◽  
Vol 209 (3) ◽  
pp. 503-504 ◽  
Author(s):  
Hiroaki Kato ◽  
Hiroshi Yamaguchi ◽  
Yasuo Hata ◽  
Takaaki Nishioka ◽  
Yukiteru Katsube ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Glutathione Synthetase ◽  
X Ray ◽  
Escherichia Coli B

Sites of Adsorption of the Bacteriophages T3 and T5 on the Wall of Escherichia Coli B.

1967 ◽  
Vol 25 ◽  
pp. 96-97
Author(s):  
Manfred E. Bayer
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Electron Microscope ◽  
Uranyl Acetate ◽  
E Coli ◽  
Receptor Sites ◽  
Rigid Cell Wall ◽  
Host Cell Surface ◽  
Bacterial Viruses ◽  
Escherichia Coli B

Bacterial viruses adsorb specifically to receptors on the host cell surface. Although the chemical composition of some of the cell wall receptors for bacteriophages of the T-series has been described and the number of receptor sites has been estimated to be 150 to 300 per E. coli cell, the localization of the sites on the bacterial wall has been unknown.When logarithmically growing cells of E. coli are transferred into a medium containing 20% sucrose, the cells plasmolize: the protoplast shrinks and becomes separated from the somewhat rigid cell wall. When these cells are fixed in 8% Formaldehyde, post-fixed in OsO4/uranyl acetate, embedded in Vestopal W, then cut in an ultramicrotome and observed with the electron microscope, the separation of protoplast and wall becomes clearly visible, (Fig. 1, 2). At a number of locations however, the protoplasmic membrane adheres to the wall even under the considerable pull of the shrinking protoplast. Thus numerous connecting bridges are maintained between protoplast and cell wall. Estimations of the total number of such wall/membrane associations yield a number of about 300 per cell.

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