scholarly journals СРАВНИТЕЛЬНЫЙ АНАЛИЗ МНОГОЛЕТНЕЙ ЭПИЗООТИЧЕСКОЙ СИТУАЦИИ ПО ОРТОХАНТАВИРУСНОЙ ИНФЕКЦИИ В ПОПУЛЯЦИЯХ APODEMUS AGRARIUS И MICROTUS FORTIS В ПРИРОДНЫХ ОЧАГАХ ЮГА ДАЛЬНЕГО ВОСТОКА РОССИИ

2018 ◽  
Vol 10 (5) ◽  
pp. 62
Author(s):  
Galina Gennadievna Kompanets ◽  
Olga Viktorovna Iunikhina
Keyword(s):  
Apodemus Agrarius

Цель. Изучить особенности эпизоотического процесса в популяциях мышевидных грызунов – носителей ортохантавирусов Hantaan и Fuzong в природных очагах инфекции Приморского края.Материалы и методы. Инфицированность ортохантавирусом 14 494 Apodemus agrarius и 2 887 Microtus fortis, отловленных в 1981–2016 гг, в разных районах края, оценивали по обнаружению специфического антигена в иммуноферментном анализе или специфических антител в непрямом методе флуоресцирующих антител (НМФА). Для более показательного сравнительного анализа многолетней динамики инфицированности в популяциях грызунов период исследования был разделен на три 12-летних периода (1981–1992 гг, 1993–2004 гг. и 2005–2016 гг.). Статистическая обработка полученных данных производилась с использованием программного обеспечения Statistica 10.0 (Statsoft, США) и статистических калькуляторов предоставленных сервисом http://medstatistic.ru.Результаты. Полученные результаты многолетней динамики инфицированности ортохантавирусом демонстрируют практически синхронные подъемы/спады инфицированности в популяциях двух видов грызунов, однако, несмотря на доминирование в отловах полевой мыши, за исследованный период отмечена статистически достоверная тенденция уменьшения активности эпизоотического процесса в её популяции. В то же время в популяции M. fortis в биотопах совместного обитания статистически достоверное снижение инфицированности отмечено только в последние 12 лет.Заключение. Таким образом, в последние годы в луго-полевых биотопах Приморского края отмечено снижение активности эпизоотического процесса в популяциях грызунов – носителей ортохантавируса Hantaan, имеющее важное значение для заболеваемости геморрагической лихорадкой с почечным синдромом в регионе.

scholarly journals Multiplex PCR-Based Nanopore Sequencing and Epidemiological Surveillance of Hantaan orthohantavirus in Apodemus agrarius, Republic of Korea

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 847
Author(s):  
Kyungmin Park ◽  
Seung-Ho Lee ◽  
Jongwoo Kim ◽  
Jingyeong Lee ◽  
Geum-Young Lee ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Republic Of Korea ◽  
Hantaan Virus ◽  
Genomic Sequences ◽  
Whole Genome ◽  
Nanopore Sequencing ◽  
Apodemus Agrarius ◽  
Copy Numbers ◽  
Nanopore Sequencer

Whole-genome sequencing of infectious agents enables the identification and characterization of emerging viruses. The MinION device is a portable sequencer that allows real-time sequencing in fields or hospitals. Hantaan orthohantavirus (Hantaan virus, HTNV), harbored by Apodemus agrarius, causes hemorrhagic fever with renal syndrome (HFRS) and poses a critical public health threat worldwide. In this study, we aimed to evaluate the feasibility of using nanopore sequencing for whole-genome sequencing of HTNV from samples having different viral copy numbers. Amplicon-based next-generation sequencing was performed in A. agrarius lung tissues collected from the Republic of Korea. Genomic sequences of HTNV were analyzed based on the viral RNA copy numbers. Amplicon-based nanopore sequencing provided nearly full-length genomic sequences of HTNV and showed sufficient read depth for phylogenetic analysis after 8 h of sequencing. The average identity of the HTNV genome sequences for the nanopore sequencer compared to those of generated from Illumina MiSeq revealed 99.8% (L and M segments) and 99.7% (S segment) identities, respectively. This study highlights the potential of the portable nanopore sequencer for rapid generation of accurate genomic sequences of HTNV for quicker decision making in point-of-care testing of HFRS patients during a hantavirus outbreak.

scholarly journals Detection of sarcocystic infection in a wild rodent (Apodemus agrarius chejuensis) captured on Jeju island

2011 ◽  
Vol 27 (4) ◽  
pp. 357 ◽  
Author(s):  
Tae-Hyoun Kim ◽  
Ju-Hee Han ◽  
Seo-Na Chang ◽  
Dong-Su Kim ◽  
Tamer Said Abdelkader ◽  
...  
Keyword(s):  
Jeju Island ◽  
Wild Rodent ◽  
Apodemus Agrarius

scholarly journals Absence of spermatozoal CD46 protein expression and associated rapid acrosome reaction rate in striped field mice (Apodemus agrarius)

2009 ◽  
Vol 7 (1) ◽  
Author(s):  
Leanne E Clift ◽  
Petra Andrlikova ◽  
Michaela Frolikova ◽  
Pavel Stopka ◽  
Josef Bryja ◽  
...  
Keyword(s):  
Protein Expression ◽  
Acrosome Reaction ◽  
Reaction Rate ◽  
Apodemus Agrarius ◽  
Field Mice

scholarly journals A novel genotype of Hantaan orthohantavirus harbored by Apodemus agrarius chejuensis as a potential etiologic agent of hemorrhagic fever with renal syndrome in Republic of Korea

2021 ◽  
Vol 15 (5) ◽  
pp. e0009400
Author(s):  
Kyungmin Park ◽  
Won-Keun Kim ◽  
Seung-Ho Lee ◽  
Jongwoo Kim ◽  
Jingyeong Lee ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Hemorrhagic Fever ◽  
Etiologic Agent ◽  
Jeju Island ◽  
Etiological Agent ◽  
Republic Of Korea ◽  
Hantaan Virus ◽  
Genomic Sequences ◽  
Mortality And Morbidity ◽  
Apodemus Agrarius

Background Orthohantaviruses, causing hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome, pose a significant public health threat worldwide. Despite the significant mortality and morbidity, effective antiviral therapeutics or vaccines for orthohantavirus infections are currently unavailable. This study aimed to investigate the prevalence of HFRS-associated orthohantaviruses and identify the etiological agent of orthohantavirus outbreaks in southern Republic of Korea (ROK). Methodology/Principal findings We collected small mammals on Jeju Island during 2018–2020. We detected the Hantaan virus (HTNV)-specific antibodies and RNA using an indirect immunofluorescence assay test and reverse transcription-polymerase chain reaction on Apodemus agrarius chejuensis (A. chejuensis). The prevalence of anti-HTNV antibodies among rodents was 14.1%. A total of six seropositive mice harbored HTNV RNA. The amplicon-based next-generation sequencing provided nearly full-length tripartite genomic sequences of six HTNV harbored by A. chejuensis. Phylogenetic and tanglegram analyses were conducted for inferring evolutionary relationships between orthohantaviruses with their reservoir hosts. Phylogenetic analyses identified a novel distinct HTNV genotype. The detected HTNV genomic sequences were phylogenetically related to a viral sequence derived from HFRS patient in southern ROK. Tanglegram analysis demonstrated the segregation of HTNV genotypes corresponding to Apodemus spp. divergence. Conclusions/Significance Our results suggest that A. chejuensis-borne HTNV may be a potential etiological agent of HFRS in southern ROK. Ancestral HTNV may infect A. chejuensis prior to geological isolation between the Korean peninsula and Jeju Island, supporting the co-evolution of orthohantaviruses and rodents. This study arises awareness among physicians for HFRS outbreaks in southern ROK.

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