Absence of spermatozoal CD46 protein expression and associated rapid acrosome reaction rate in striped field mice (Apodemus agrarius)

SummarySpermatozoa obtained from hybrid male mice were treated with solubilised zonae pellucidae after a period of capacitation in vitro to induce the acrosome reaction. Single spermatozoa were selected and microinjected into the perivitelline space of mature oocytes. A high proportion of the spermatozoa acrosome-reacted affter treatment with solubilised zonae (63%). However, the fertilisation rate (37%) after subzonal microinjection of oocytes by the treated spermatozoa was not different to the fertilisation rate (40%) of oocytes microinjected with untreated spermatozoa which had a lower rate of acrosome reaction (39%). When spermatozoa were washed by high-speed centrifugation before treatment with solubilised zonae pellucidae and subzonal microinjection, the fertilisation rate (68%) was significantly higher (p < 0.001) than that (29%) for occytes microinjected with untreated spermatozoa and was found to be correlated with a high acrosome reaction rate (74%) (r = 0.8). The washing of spermatozoa by centrifugation itself did not increase the acrosome reaction rate or fertilisation rate of oocytes after microinjection. The results of this study suggests that some modifications other than the acrosome reaction are needed to enable capacitated mouse spermatozoa to fuse with the oocyte plasma membrane. These modifications were achieved by washing spermatozoa by high-speed centrifugation and the replacement of the supernatant with fresh culture medium used for capacitation. Induction of the acrosome reaction by solubilised zonae pellucidae following this treatment leads to a high fertilisation rate of oocytes by subzonal sperm microinjection.

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Seasonal changes in gut morphology of the striped field mouse (Apodemus agrarius)

Canadian Journal of Zoology ◽

10.1139/z95-130 ◽

1995 ◽

Vol 73 (6) ◽

pp. 1095-1099 ◽

Cited By ~ 13

Author(s):

Anetta Borkowska

Keyword(s):

Small Intestine ◽

Surface Area ◽

Seasonal Changes ◽

Poor Quality ◽

Mucosal Surface ◽

Apodemus Agrarius ◽

Late Autumn ◽

Field Mice ◽

Highly Correlated ◽

Mucosal Surface Area

I studied seasonal changes in length and wet mass of the digestive tract and area of the mucosal surface of the small intestine in wild adult striped field mice (Apodemus agrarius). The length and mass of the gut varied with season in males but not females. The mucosal surface area of the small intestine was highly correlated with seasonal variations in the size of the alimentary tract. Changes in the surface area of the mucosa depended on changes in both villus size and the number of villi per unit area. The area of the intestinal mucosa varied during the year in both females and males, but unexpectedly, the mucosal surface area in females was significantly larger than in males only in autumn. Because gut size decreased toward late autumn, I suggest that late autumn was not a stressful period (i.e., because of low temperature or poor-quality food) for wild field mice. The decrease in gut parameters may have been an effect of declining metabolic requirements and completion of reproduction in late autumn.

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Progress of sperm IZUMO1 relocation during spontaneous acrosome reaction

Reproduction ◽

10.1530/rep-13-0193 ◽

2014 ◽

Vol 147 (2) ◽

pp. 231-240 ◽

Cited By ~ 18

Author(s):

Natasa Sebkova ◽

Lukas Ded ◽

Katerina Vesela ◽

Katerina Dvorakova-Hortova

Keyword(s):

Fusion Protein ◽

Acrosome Reaction ◽

Mating Behaviour ◽

Calcium Ionophore ◽

Cumulus Cells ◽

Sperm Head ◽

High Rate ◽

Field Mice ◽

Species Specific

It has been recently shown in mice that sperm undergo acrosome reaction (AR) by passing through cumulus cells; furthermore, the acrosome-reacted sperm can bind to zona pellucida and consequently fertilise the egg. During AR, the relocation of the primary fusion protein IZUMO1 into the equatorial segment is crucial for sperm–egg fusion. There is a high rate of spontaneous AR in rodents, with up to 60% in promiscuous species. The aim of this study was to clarify whether the IZUMO1 relocation in sperm after spontaneous and induced AR is the same, and whether there is a correlation between the speed of IZUMO1 relocation and species-specific mating behaviour in field mice. Immunofluorescent detection of IZUMO1 dynamics during the in vitro capacitation, spontaneous, calcium ionophore and progesterone-induced AR was monitored. Our results show that during spontaneous AR, there is a clear IZUMO1 relocation from the acrosomal cap to the equatorial segment, and further over the whole sperm head. In addition, there is positive tail tyrosine phosphorylation (TyrP) associated with hyperactive motility. Moreover, the beginning and the progress of IZUMO1 relocation and tail TyrP positively correlate with the level of promiscuity and the acrosome instability in promiscuous species. The findings that crucial molecular changes essential for sperm–egg fusion represented by dynamic movements of IZUMO1 also happen during spontaneous AR are vital for understanding fertilisation in mice.

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Morphological and Molecular Characterization of Toxocara apodemi (Nematoda: Ascarididae) from Striped Field Mice, Apodemus agrarius, in Korea

The Korean Journal of Parasitology ◽

10.3347/kjp.2020.58.4.403 ◽

2020 ◽

Vol 58 (4) ◽

pp. 403-411

Author(s):

Hyeon Cheol Kim ◽

Eui Ju Hong ◽

Si Yun Ryu ◽

Jinho Park ◽

Jeong Gon Cho ◽

...

Keyword(s):

Electron Microscope ◽

Scanning Electron Microscope ◽

18S Rrna ◽

Rrna Gene ◽

Scanning Electron Micrographs ◽

Toxocara Cati ◽

Apodemus Agrarius ◽

Toxocara Vitulorum ◽

Field Mice ◽

Scanning Electron

Adult ascarid worms from the field mice, Apodemus agrarius, were observed with a light and scanning electron microscope, and molecularly analized with 18S rRNA gene. In the scanning electron microscope, 3 prominent labia were present in the anterior end of male and female worms, but the interlabia and gubernaculum were absent. Scanning electron micrographs showed cervical alae as vestigial organs that looked like a slightly uplifted superficial sewing stitch. Total 6 pairs of post-cloacal papillae were observed on the tail of the male worms. The tail of female worms was blunt and conical shape with a spine-like structure, mucron. The eggs were sub-globular, coated with the albuminous layer and 73 by 82 μm in average size. The superficial pits of T. apodemi egg (mean 8.6×6.7 μm) are obviously bigger than those of Toxocara spp. The partial sequence of 18S rRNA showed the sequence homology of Toxocara canis (99.6%), Toxocara cati (99.4%), Toxascaris leonina (99.4%), and Toxocara vitulorum (99.2%). Conclusively, it was confirmed that ascarid nematodes, Toxocara apodemi, recovered from striped field mice in Korea are taxonomically conspecific relationship with genus Toxocara and genetic divergence from other Toxocara species.

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Infections of Intestinal Helminth at Two Species of Field Mice, Apodemus agrarius and A. Peninsulae, in Gangwondo and Chungcheongnam-do, Korea

The Korean Journal of Parasitology ◽

10.3347/kjp.2018.56.3.301 ◽

2018 ◽

Vol 56 (3) ◽

pp. 301-304

Author(s):

Jae-Hyung Lee ◽

Shuang Gong ◽

Yung Chul Park ◽

Hyun-Ju Kim ◽

In-Wook Choi ◽

...

Keyword(s):

Intestinal Helminth ◽

Apodemus Agrarius ◽

Field Mice

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Study of Catsper1 Protein Levels in Unexplained and Idiopathic Infertile Men

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.v9i2.13646 ◽

2018 ◽

Vol 9 (2) ◽

Cited By ~ 1

Author(s):

Hayder L F AL-Msaid ◽

Alaauldeen S M AL-Sallami

Keyword(s):

Protein Expression ◽

Acrosome Reaction ◽

Sperm Concentration ◽

Unexplained Infertility ◽

Normal Morphology ◽

Positive Correlation ◽

Protein Levels ◽

Progressive Motility ◽

Infertile Men ◽

Protein Expressions

Catsper 1 is an important protein involved in calcium channel synthesis. These channels are essential for the hyperactivity of sperm flagellum, chemotaxis towards the egg, capacitation and acrosome reaction. Data analysis from unexplained infertility (n=50), idiopathic infertile men (Asthenozoospermia (n=50), Oligozoospermia (n=50)), and Normospermia fertile men as a control (n=26). The expression of CatSper1 protein expression in spermatozoa was reduced in unexplained infertility (mean± SD. Error 64.32±2.5 and in Asthenozoospermia 46.6±2.1and Oligozoospermia 53.86±4.1 while significant increase was observed in Normospermia fertile men 115.19±2.3. CatSper1 protein expressions was shown to have a positive correlation between sperm concentration, Sperm Progressive motility percentage and sperm normal morphology present in idiopathic infertile men respectively (r= 0.822, r = 0.651 and r = 0.742). A positive correlation was found between CatSper1 protein expression and sperm concentration, Sperm Progressive motility percentage and sperm normal morphology present in unexplained infertile men Respectively (r=0.845, r = 0.576 and r = 0.582). The present study shows the importance of protein catsper 1 in infertile men and its positive correlation with sperm parameters.

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