Metabolic Activity of Multipotent Mesenchymal Stromal Cells by Means of Moification of Ph-Condition of Incubation and the L-Carnitine Effects

Culture of cell lines have been used traditionally to evaluate medicinal cytotoxicity. The aim of this study was to evaluate the effect of L-carnitine on the metabolic activity of multipotent mesenchymal stromal cells. Research methods. The object of the study was the culture of multipotent mesenchymal stromal cells of the 6th passage derived from adipocytes in rat. Cultivation of this cell line was carried out at the optimum pH-condition (7.37) and at the modification of the values (4.0, 5.5 and 9.0). The MTT-test was performed by the method of M. Niks and M.Otto in modification. Results. The research culture of cells, it is shown that the simulation acidosis (pH 4.0 and 5.5) and alkalosis (pH of 9.0) accompanied by a decrease in the intensity of their metabolism by MTT test. The severity of oppression activity of mitochondrial dehydrogenases significantly depended on the magnitude of deviations from normal pH values. Introduction in the environment of cultivation L-carnitine in the range of concentrations 10-3-10-10 Mol/1 increases the intensity of formation of theformazan salts all over the pH range of incubation medium. The optimal effect of L-carnitine manifests in concentrations 10-5-10-6 Mol/1 and with the least deviation of the pH of the environment from the normal values. The discussion. The obtained results confirm the hypothesis about the ability of L-carnitine to maintain the metabolism of cells in the failure of intra - and extracellular pH caused by stress, hypoxia, strenuous and ischemia. In the conditions and acidosis and alkalosis L- carnitine stimulates the synthesis of ATP through participation in the oxidation of free fatty acids into the mitochondria. This property of the drug may explain some of its pharmacological effects and is of interest for specialists in the field of sports medicine.