Radiological and Histopathological Study of Diabetes Mellitus Effect on Bone Healing in Late Stages in Dogs

2015 ◽  
Vol 10 (2) ◽  
pp. 86-93
Author(s):  
Abdulbari A. Al-Faris ◽  
Alaa A. Sawad ◽  
Saad H. Zebon
Keyword(s):  
Diabetes Mellitus ◽  
Bone Healing ◽  
Histopathological Study ◽  
Late Stages

scholarly journals Study of nano-hydroxyapatite/zirconia stabilized with yttria in bone healing: histopathological study in rabbit model

2014 ◽  
Vol 1 ◽  
pp. 40-47 ◽  
Author(s):  
Gholamreza Abedi ◽  
Amirali Jahanshahi ◽  
Mohamad Hosein Fathi ◽  
Iraj Sohrabi Haghdost ◽  
Abas Veshkini
Keyword(s):  
Bone Healing ◽  
Rabbit Model ◽  
Histopathological Study

Efficacy of Ankaferd Blood Stopper on bone healing in diabetic rats: a stereological and histopathological study

2014 ◽  
Vol 89 (7) ◽  
pp. 535-543 ◽  
Author(s):  
E Bulut ◽  
B Baş ◽  
BZ Altunkaynak ◽  
B Bekçioğlu ◽  
G Erdem Koç ◽  
...  
Keyword(s):  
Bone Healing ◽  
Diabetic Rats ◽  
Histopathological Study ◽  
Ankaferd Blood Stopper

scholarly journals Diabetes-associated alterations in the cecal microbiome and metabolome are independent of diet or environment in the UC Davis Type 2 Diabetes Mellitus Rat model

2018 ◽  
Vol 315 (5) ◽  
pp. E961-E972 ◽  
Author(s):  
Brian D. Piccolo ◽  
James L. Graham ◽  
Kimber L. Stanhope ◽  
Intawat Nookaew ◽  
Kelly E. Mercer ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Gut Microbiome ◽  
Bacterial Species ◽  
Dietary Factors ◽  
Metagenomic Sequencing ◽  
Bacterial Gene ◽  
Late Stages

The composition of the gut microbiome is altered in obesity and type 2 diabetes; however, it is not known whether these alterations are mediated by dietary factors or related to declines in metabolic health. To address this, cecal contents were collected from age-matched, chow-fed male University of California, Davis Type 2 Diabetes Mellitus (UCD-T2DM) rats before the onset of diabetes (prediabetic PD; n = 15), 2 wk recently diabetic (RD; n = 10), 3 mo (D3M; n = 11), and 6 mo (D6M; n = 8) postonset of diabetes. Bacterial species and functional gene counts were assessed by shotgun metagenomic sequencing of bacterial DNA in cecal contents, while metabolites were identified by gas chromatography-quadrupole time-off-flight-mass spectrometry. Metagenomic analysis showed a shift from Firmicutes species in early stages of diabetes (PD + RD) toward an enrichment of Bacteroidetes species in later stages of diabetes (D3M + D6M). In total, 45 bacterial species discriminated early and late stages of diabetes with 25 of these belonging to either Bacteroides or Prevotella genera. Furthermore, 61 bacterial gene clusters discriminated early and later stages of diabetes with elevations of enzymes related to stress response (e.g., glutathione and glutaredoxin) and amino acid, carbohydrate, and bacterial cell wall metabolism. Twenty-five cecal metabolites discriminated early vs. late stages of diabetes, with the largest differences observed in abundances of dehydroabietic acid and phosphate. Alterations in the gut microbiota and cecal metabolome track diabetes progression in UCD-T2DM rats when controlling for diet, age, and housing environment. Results suggest that diabetes-specific host signals impact the ecology and end product metabolites of the gut microbiome when diet is held constant.

scholarly journals Local Application of Semaphorin 3A Combined with Adipose-Derived Stem Cell Sheet and Anorganic Bovine Bone Granules Enhances Bone Regeneration in Type 2 Diabetes Mellitus Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Xiaoru Xu ◽  
Kaixiu Fang ◽  
Lifeng Wang ◽  
Xiangwei Liu ◽  
Yuchao Zhou ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Stem Cell ◽  
Bone Healing ◽  
Cell Sheet ◽  
Semaphorin 3A ◽  
Adipose Derived Stem Cell

Bone tissue regeneration is considered to be the optimal solution for bone loss. However, diabetic patients have a greater risk of poor bone healing or bone grafting failure than nondiabetics. The purpose of this study was to investigate the influence of the complexes of an adipose-derived stem cell sheet (ASC sheet) and Bio-Oss® bone granules on bone healing in type 2 diabetes mellitus (T2DM) rats with the addition of semaphorin 3A (Sema3A). The rat ASC sheets showed stronger osteogenic ability than ASCs in vitro, as indicated by the extracellular matrix mineralization and the expression of osteogenesis-related genes at mRNA level. An ASC sheet combined with Bio-Oss® bone granules promoted bone formation in T2DM rats as indicated by microcomputed tomography (micro-CT) and histological analysis. In addition, Sema3A promoted the osteogenic differentiation of ASC sheets in vitro and local injection of Sema3A promoted T2DM rats’ calvarial bone regeneration based on ASC sheet and Bio-Oss® bone granule complex treatment. In conclusion, the local injection of Sema3A and the complexes of ASC sheet and Bio-Oss® bone granules could promote osseous healing and are potentially useful to improve bone healing for T2DM patients.

Type 2 diabetes mellitus impairs bone healing of dental implants in GK rats

2010 ◽  
Vol 88 (1) ◽  
pp. e7-e9 ◽  
Author(s):  
Feng Wang ◽  
Ying-liang Song ◽  
De-hua Li ◽  
Cui-xia Li ◽  
Yao Wang ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Dental Implants ◽  
Bone Healing ◽  
Gk Rats

Impact of diabetes mellitus and metabolic control on bone healing around osseointegrated implants: removal torque and histomorphometric analysis in rats

2012 ◽  
Vol 24 (7) ◽  
pp. 831-837 ◽  
Author(s):  
Rafael Scaf de Molon ◽  
Juliana Aparecida Najarro Dearo Morais-Camilo ◽  
Mario Henrique Arruda Verzola ◽  
Rafael Silveira Faeda ◽  
Maria Teresa Pepato ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Metabolic Control ◽  
Bone Healing ◽  
Histomorphometric Analysis ◽  
Removal Torque ◽  
Osseointegrated Implants

scholarly journals Effect of Post-Operative Toradol Administration on Bone Healing After Ankle Fracture Fixation

2017 ◽  
Vol 2 (3) ◽  
pp. 2473011417S0002
Author(s):  
Elizabeth McDonald ◽  
Brian Winters ◽  
Rachel Shakked ◽  
David Pedowitz ◽  
Steven Raikin ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Tobacco Use ◽  
Bone Healing ◽  
Historical Control ◽  
Lateral Malleolus ◽  
Ankle Fractures ◽  
Medial Malleolus ◽  
Foot And Ankle ◽  
Factors Associated ◽  
Posterior Malleolus

Category: Ankle, Trauma Introduction/Purpose: Ketorolac has been reported to delay bone healing when administered after spine surgery, and there is hesitancy to use non-steroidal anti-inflammatories (NSAIDs) in the fracture setting despite its reliable ability to relieve surgical pain. The effect of ketorolac administration after foot and ankle surgery has not been well-defined in the literature to date. The purpose of this study is to report clinical and radiographic outcomes for patients treated with a perioperative ketorolac regimen after open reduction and internal fixation (ORIF) of ankle fractures. A secondary purpose is to determine whether there are other patient factors that affect radiographic healing in this population. We hypothesize that the time to radiographic healing with ketorolac use after ORIF of ankle fractures is no different than a historical control. Methods: A retrospective chart review was performed on all patients that received perioperative ketorolac at the time of lateral malleolar, bimalleolar, and trimalleolar ankle ORIF by a single surgeon between 2010 and 2016 with minimum 4 months follow-up. Patients were prescribed 5 days of 10 mg ketorolac every 6 hours. Radiographs were evaluated independently by two blinded fellowship-trained foot and ankle surgeons to assess for radiographic healing of lateral malleolus, medial malleolus, and posterior malleolus fractures at 6, 12, and 16 weeks post-operatively. Two hundred and ninety-four patients were included with an average age of 50 years with 138 males (47%). Literature review was performed to determine an appropriate historical control of time to radiographic healing after ankle ORIF for comparison. Statistical analysis consisted of a linear mixed-effects regression which was performed to estimate the effect of time and covariates, taking into account repeated measurements on the same subject. Results: Radiographic healing was demonstrated by 16 weeks in 221 of 281 (79%) lateral malleolus fractures, 105 of 132 medial malleolus fractures (80%), and 53 of 57 (93%) posterior malleolus fractures (see Figure 1). Median healing times were 12, 11, and 6 weeks for lateral, medial, and posterior malleoli fractures respectively. There was no significant difference in time to radiographic healing of lateral malleolus when compared to a historical control of 16.7 weeks to union. Active tobacco use was an independent risk factor for delayed radiographic healing (p < 0.05). Diabetes mellitus and age greater than 50 years were independent factors associated with faster healing of the lateral malleolus fractures (p < 0.05). Rheumatoid arthritis, oral steroid use, and obesity had no effect on radiographic healing. Conclusion: Perioperative ketorolac use did not affect radiographic healing of ankle fractures after ORIF. As expected, active tobacco use was associated with slower radiographic healing. There is no evidence that ketorolac use further delayed union in smokers, but this may warrant further study. We unexpectedly identified diabetes mellitus and older age as factors associated with faster healing which also warrants further study. This is the first study to date examining the effect of ketorolac on radiographic time to union of ankle fractures. Further study may help determine whether ketorolac helps reduce opioid consumption and improve pain following ORIF of ankle fractures.

scholarly journals Onychomycosis in patients with diabetes mellitus: Etiology, clinical features, and treatment response

2021 ◽  
Vol 12 (4) ◽  
pp. 359-366
Author(s):  
Alma Ileana Molina-Hernandez ◽  
Hassiel Aurelio Ramírez-Marín ◽  
Alexandro Bonifaz
Keyword(s):  
Diabetes Mellitus ◽  
Treatment Response ◽  
Correct Diagnosis ◽  
Response To Therapy ◽  
Histopathological Study ◽  
Direct Examination ◽  
Calcofluor White ◽  
Nail Diseases ◽  
Pharmacological Interactions ◽  
Patients With Diabetes

Background: Onychomycosis accounts for 30% of all superficial mycoses and 50% of all nail diseases. One of the most studied predisposing factors is diabetes mellitus, with a frequency of onychomycosis of 31.5% in these patients. Many show resistance to standard therapeutics and have “polypharmacy”, which represents a risk for pharmacological interactions. Objective: The objective was to assess the clinical response to therapy, evaluate with histopathology, direct examination with KOH and white-calcofluor, and culture the most frequent etiologic agents associated with the development of onychomycosis in patients with diabetes mellitus. Materials and Methods: A non-randomized, uncontrolled, open-ended, prospective cohort study was conducted on 46 patients with onychomycosis and diabetes mellitus. Treatment was assigned according to clinical findings and specific indications for treatment. Results: From the samples taken for direct examination with KOH and calcofluor-white, culture, and histopathological study, positive results were: 39 (84.1%) patients to the direct examination, 32 (69.6%) to the culture, 27 (65.2%) with a positive histopathological study, and 17 (54.86%) to the calcofluor-white. On clinical evaluation, we found no treatment response in 8 patients (20%), a partial response in 14 patients (25%), and a complete response in 18 patients (45%). Out of the 46 patients evaluated initially, 25 persisted with onychomycosis after six months of follow-up. Conclusion: The prevalence of onychomycosis is increasing and requires correct diagnosis since there are other non-fungal diseases of the nails that resemble onychomycosis. Presumably, the immunosuppression of diabetes, its systemic affection, and the foot abnormalities of a diabetic patient cause more nail dystrophy, an increased fungal load, and treatment resistance.

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