scholarly journals Molecular Typing of Avian Pathogenic Escherichia coli O78 Strains in Japan by Using Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis

2010 ◽  
Vol 72 (12) ◽  
pp. E3-E3
Author(s):  
Manao OZAWA ◽  
Kotaro BABA ◽  
Tetsuo ASAI
Keyword(s):  
Escherichia Coli ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Multilocus Sequence Typing ◽  
Pulsed Field Gel Electrophoresis ◽  
Avian Pathogenic Escherichia Coli ◽  
Pulsed Field ◽  
Pathogenic Escherichia Coli

scholarly journals Molecular typing of Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan

1999 ◽  
Vol 122 (2) ◽  
pp. 337-341 ◽  
Author(s):  
M. AKIBA ◽  
T. MASUDA ◽  
T. SAMESHIMA ◽  
K. KATSUDA ◽  
M. NAKAZAWA
Keyword(s):  
Escherichia Coli ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Escherichia Coli O157 ◽  
Outbreak Strain ◽  
Pulsed Field ◽  
E Coli ◽  
Biological Methods ◽  
Pfge Profiles

A total of 77 Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan were investigated by molecular biological methods. Most of these isolates (43 isolates) possessed the stx2 gene, but not stx1. Fifteen bacteriophage types and 50 pulsed-field gel electrophoresis (PFGE) profiles were observed. One isolate was indistinguishable from the human outbreak strain by these methods. This indicates that cattle must be considered as a possible source of human E. coli O157[ratio ]H7 infection in Japan.

scholarly journals Genetic Characterization of Atypical Enteropathogenic Escherichia coli Isolates from Ewes' Milk, Sheep Farm Environments, and Humans by Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis

2013 ◽  
Vol 79 (19) ◽  
pp. 5864-5869 ◽  
Author(s):  
Verónica Otero ◽  
José-María Rodríguez-Calleja ◽  
Andrés Otero ◽  
María-Luisa García-López ◽  
Jesús A. Santos
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Multilocus Sequence Typing ◽  
Pulsed Field Gel Electrophoresis ◽  
Content Type ◽  
Pulsed Field ◽  
Atypical Epec ◽  
Sheep Farm ◽  
Stool Samples ◽  
Human Stool

ABSTRACTA collection of 81 isolates of enteropathogenicEscherichia coli(EPEC) was obtained from samples of bulk tank sheep milk (62 isolates), ovine feces (4 isolates), sheep farm environment (water, 4 isolates; air, 1 isolate), and human stool samples (9 isolates). The strains were considered atypical EPEC organisms, carrying theeaegene without harboring the pEAF plasmid. Multilocus sequence typing (MLST) was carried out with seven housekeeping genes and 19 sequence types (ST) were detected, with none of them having been previously reported for atypical EPEC. The most frequent ST included 41 strains isolated from milk and human stool samples. Genetic typing by pulsed-field gel electrophoresis (PFGE) resulted in 57 patterns which grouped in 24 clusters. Comparison of strains isolated from the different samples showed phylogenetic relationships between milk and human isolates and also between milk and water isolates. The results obtained show a possible risk for humans due to the presence of atypical EPEC in ewes' milk and suggest a transmission route for this emerging pathogen through contaminated water.

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