scholarly journals Genetic Characterization of Atypical Enteropathogenic Escherichia coli Isolates from Ewes' Milk, Sheep Farm Environments, and Humans by Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis

2013 ◽  
Vol 79 (19) ◽  
pp. 5864-5869 ◽  
Author(s):  
Verónica Otero ◽  
José-María Rodríguez-Calleja ◽  
Andrés Otero ◽  
María-Luisa García-López ◽  
Jesús A. Santos
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Multilocus Sequence Typing ◽  
Pulsed Field Gel Electrophoresis ◽  
Content Type ◽  
Pulsed Field ◽  
Atypical Epec ◽  
Sheep Farm ◽  
Stool Samples ◽  
Human Stool

ABSTRACTA collection of 81 isolates of enteropathogenicEscherichia coli(EPEC) was obtained from samples of bulk tank sheep milk (62 isolates), ovine feces (4 isolates), sheep farm environment (water, 4 isolates; air, 1 isolate), and human stool samples (9 isolates). The strains were considered atypical EPEC organisms, carrying theeaegene without harboring the pEAF plasmid. Multilocus sequence typing (MLST) was carried out with seven housekeeping genes and 19 sequence types (ST) were detected, with none of them having been previously reported for atypical EPEC. The most frequent ST included 41 strains isolated from milk and human stool samples. Genetic typing by pulsed-field gel electrophoresis (PFGE) resulted in 57 patterns which grouped in 24 clusters. Comparison of strains isolated from the different samples showed phylogenetic relationships between milk and human isolates and also between milk and water isolates. The results obtained show a possible risk for humans due to the presence of atypical EPEC in ewes' milk and suggest a transmission route for this emerging pathogen through contaminated water.

Occurrence of extended-spectrum β-lactamase, AmpC and virulence genes in Escherichia coli isolates from vegetable salads in Morocco

2017 ◽  
Vol 119 (7) ◽  
pp. 1633-1647
Author(s):  
Kaotar Nayme ◽  
Abouddihaj Barguigua ◽  
Brahim Bouchrif ◽  
Idrissa Diawara ◽  
Fatima El Otmani ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Virulence Genes ◽  
Phylogenetic Group ◽  
Pulsed Field Gel Electrophoresis ◽  
Resistant Bacteria ◽  
Content Type ◽  
Pulsed Field ◽  
E Coli ◽  
Extended Spectrum

Purpose The purpose of this paper is to assess the occurrence of the extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamase genes in 144 Escherichia coli isolates recovered from 160 vegetable salad samples. Design/methodology/approach Among the 144 E. coli isolates recovered from 160 vegetable salads, 17 (12 percent) ceftazidime-resistant isolates were screened for ESBL production with the double disk-diffusion test. The ESBL-producing isolates were characterized for antimicrobial resistance, the presence of virulence genes and plasmid-mediated quinolone resistance (PMQR) determinants. The isolates were also subjected to phylogenetic group typing. The existence of plasmid AmpC genes and mutations in the regulatory region of the chromosomal AmpC gene was assessed using polymerase chain reaction (PCR) and sequencing. All β-lactamase isolates were further characterized by pulsed-field gel electrophoresis to determine the genetic relatedness. Findings Overall, 17 (12 percent) of the 144 E. coli isolates studied were ceftazidime resistant. Among the 17 isolates, 13 (77 percent) were multidrug resistant and four (23.5 percent) were ESBL producers. The bla CTX-M14 was the only gene detected. Of the 12 AmpC-producing isolates, three (18 percent) harbored plasmid-encoded AmpC and sequencing analysis of the chromosomal AmpC genes revealed mutations in the promoter/attenuator region. PMQR determinants were detected in 9 (52 percent) isolates. A was the most prevalent phylogenetic group (56 percent), followed by groups B1 (31 percent), D (6 percent), and B2 (6 percent). PCR showed that six (50 percent) ESBL/AmpC-producing E. coli isolates carried one and/or two virulence genes. Pulsed-field gel electrophoresis showed no epidemiological relationship between these isolates. Originality/value This study places vegetable salads within the spectrum of ecological niches that may be vehicles for antibiotic-resistant bacteria/genes with clinical interest and these findings are worthy of attention as their spread to humans by ingestion cannot be dismissed.

scholarly journals Detection of Carbapenemase Genes in Aquatic Environments in Rio de Janeiro, Brazil

2016 ◽  
Vol 60 (7) ◽  
pp. 4380-4383 ◽  
Author(s):  
Carlos Felipe Machado de Araujo ◽  
Dalton Marcondes Silva ◽  
Marcos Tavares Carneiro ◽  
Sthefanie Ribeiro ◽  
Marcela Fontana-Maurell ◽  
...  
Keyword(s):  
Water Samples ◽  
Gel Electrophoresis ◽  
Multilocus Sequence Typing ◽  
Rio De Janeiro ◽  
Pulsed Field Gel Electrophoresis ◽  
Aquatic Environments ◽  
Gene Pools ◽  
Content Type ◽  
Pulsed Field

ABSTRACTThis study reveals the presence of different carbapenemase genes (blaKPC,blaNDM,blaGES, andblaOXA48-likegenes) detected directly from water samples and clonal dispersion (by pulsed-field gel electrophoresis [PFGE] and multilocus sequence typing [MLST]) of KPC-2-producingEnterobacteriaceaein two important urban aquatic matrixes from Rio de Janeiro, Brazil, highlighting the role of aquatic environments as gene pools and the possibility of community spreading.

scholarly journals Extraintestinal Pathogenic and Antimicrobial-Resistant Escherichia coli Contamination of 56 Public Restrooms in the Greater Minneapolis-St. Paul Metropolitan Area

2015 ◽  
Vol 81 (13) ◽  
pp. 4498-4506 ◽  
Author(s):  
Muhanad Mohamed ◽  
Kris Owens ◽  
Abby Gajewski ◽  
Connie Clabots ◽  
Brian Johnston ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metropolitan Area ◽  
Gel Electrophoresis ◽  
Virulence Gene ◽  
Pulsed Field Gel Electrophoresis ◽  
Ecological Data ◽  
Content Type ◽  
Pulsed Field ◽  
E Coli ◽  
Sequence Types

ABSTRACTHow extraintestinal pathogenicEscherichia coli(ExPEC) and antimicrobial-resistantE. colidisseminate through the population is undefined. We studied public restrooms for contamination withE. coliand ExPEC in relation to source and extensively characterized theE. coliisolates. For this, we cultured 1,120 environmental samples from 56 public restrooms in 33 establishments (obtained from 10 cities in the greater Minneapolis-St. Paul, MN, metropolitan area in 2003) forE. coliand compared ecological data with culture results. Isolates underwent virulence genotyping, phylotyping, clonal typing, pulsed-field gel electrophoresis (PFGE), and disk diffusion antimicrobial susceptibility testing. Overall, 168 samples (15% from 89% of restrooms) fluoresced, indicating presumptiveE. coli: 25 samples (2.2% from 32% of restrooms) yieldedE. coliisolates, and 10 samples (0.9% from 16% of restrooms) contained ExPEC. Restroom category and cleanliness level significantly predicted only fluorescence, gender predicted fluorescence andE. coli, and feces-like material and toilet-associated sites predicted all three endpoints. Of the 25E. coliisolates, 7 (28%) were from phylogenetic group B2(virulence-associated), and 8 (32%) were ExPEC. ExPEC isolates more commonly represented group B2 (50% versus 18%) and had significantly higher virulence gene scores than non-ExPEC isolates. Six isolates (24%) exhibited ≥3-class antibiotic resistance, 10 (40%) represented classic human-associated sequence types, and one closely resembled reference human clinical isolates by pulsed-field gel electrophoresis. Thus,E. coli, ExPEC, and antimicrobial-resistantE. colisporadically contaminate public restrooms, in ways corresponding with restroom characteristics and within-restroom sites. Such restroom-sourceE. colistrains likely reflect human fecal contamination, may pose a health threat, and may contribute to population-wide dissemination of such strains.

scholarly journals Carriage of Escherichia coli Producing CTX-M-Type Extended-Spectrum β-Lactamase in Healthy Vietnamese Individuals

2015 ◽  
Vol 59 (10) ◽  
pp. 6611-6614 ◽  
Author(s):  
Thi Mai Huong Bui ◽  
Itaru Hirai ◽  
Shuhei Ueda ◽  
Thi Kim Ngan Bui ◽  
Kouta Hamamoto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Fecal Samples ◽  
Content Type ◽  
Pulsed Field ◽  
E Coli ◽  
Extended Spectrum

ABSTRACTHealthy carriage of CTX-M-type extended-spectrum β-lactamase (ESBL)-producingEscherichia coliwas examined by thrice collecting fecal samples from the same 199 healthy Vietnamese subjects every 6 months. Using pulsed-field gel electrophoresis (PFGE), identical PFGE patterns throughout the three samplings were not observed, although prevalence ofE. coliin the subjects was around 50% in the three samplings. Our results suggested a short carriage period of the CTX-M-type ESBL-producingE. coliin healthy Vietnamese subjects.

scholarly journals Prevalence and Relatedness of Escherichia coli O157:H7 Strains in the Feces and on the Hides and Carcasses of U.S. Meat Goats at Slaughter

2013 ◽  
Vol 79 (13) ◽  
pp. 4154-4158 ◽  
Author(s):  
M. E. Jacob ◽  
D. M. Foster ◽  
A. T. Rogers ◽  
C. C. Balcomb ◽  
M. W. Sanderson
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Processing Plant ◽  
Escherichia Coli O157 ◽  
Content Type ◽  
Pulsed Field ◽  
E Coli ◽  
Meat Goats ◽  
Multiple Sample

ABSTRACTWe determined the prevalences ofEscherichia coliO157:H7 in feces, hide, and carcasses of meat goats at a U.S. processing plant. Prevalences were 11.1%, 2.7%, and 2.7%, respectively. Sixteen pulsed-field gel electrophoresis (PFGE) subtypes were identified among 49E. coliO157:H7 isolates, some of which were present on multiple sample types or collection days.

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