scholarly journals Preliminary Experiment for Detection of Penicillinase by Enzyme-Linked Immunosorbent Assay and Western Blotting Technique.

1992 ◽  
Vol 54 (2) ◽  
pp. 395-397
Author(s):  
Shin-ichi KAMATA ◽  
Atsushi OHKAWA ◽  
Osamu ITO ◽  
Norihide KAKIICHI ◽  
Kenichi KOMINE ◽  
...  
Keyword(s):  
Western Blotting ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blotting Technique

A Comparison of an Enzyme Linked Immunosorbent Assay (ELISA) and Western Blotting for Detection of Peanut Mottle Virus and Peanut Stripe Virus1

1986 ◽  
Vol 13 (2) ◽  
pp. 64-67 ◽  
Author(s):  
J. L. Sherwood ◽  
H. A. Melouk
Keyword(s):  
Western Blotting ◽  
Immunosorbent Assay ◽  
Protein A ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Coat Proteins ◽  
Western Blots ◽  
Peanut Stripe Virus ◽  
Dry Milk ◽  
The Difference

Abstract Western blotting was used to detect infections of peanut cv. Tamnut 74 with peanut mottle virus (PMV) and/or peanut stripe virus (PStV). Leaf samples were ground in electrophoresis sample buffer and heated for 5 min at 95 C prior to electrophoresis in 12% polyacrylamide gels. After electrophoresis, proteins were transferred to nitrocellulose sheets at 100V for 45 min. Western blots were performed by first blocking unbound sites on the nitrocellulose with 5% non-fat dry milk in Tris-buffered saline (TBS), pH 7.4 for 30 min, followed by incubation in a 1/200 dilution of PMV and/or PStV antiserum in TBS (the latter antiserum provided by J. W. Demski, U. of GA) for 45 min. This was followed by incubation in protein-A-peroxidase (2 μg/mL in TBS) for 45 min, followed by 4-chloro-1-napthol plus hydrogen peroxide in TBS. As little as 25 ng of either purified PMV or PStV was detected. This was similar to the limits of detection fo the double sandwich enzyme linked immunosorbent assay (ELISA). Because of the difference in migration of the coat proteins of PMV and PStV, both viruses may be detected in plants infected with PMV and PStV. This assay can be performed in approximately 6 h when mini-gels are used for the initial electrophoretic seperation and does not require the antiserum to be fractionated or bound to an enzyme as is the case with ELISA.

scholarly journals Development of an Enzyme-Linked Immunosorbent Assay Based on Fusion VP2332-452Antigen for Detecting Antibodies against Aleutian Mink Disease Virus

2015 ◽  
Vol 54 (2) ◽  
pp. 439-442 ◽  
Author(s):  
Xiaowei Chen ◽  
Cailing Song ◽  
Yun Liu ◽  
Liandong Qu ◽  
Dafei Liu ◽  
...  
Keyword(s):  
Disease Virus ◽  
Western Blotting ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Reference Test ◽  
Aleutian Mink Disease Virus ◽  
Specificity And Sensitivity

For detection of Aleutian mink disease virus (AMDV) antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed using the recombinant VP2332-452protein as an antigen. Counterimmunoelectrophoresis (CIEP) was used as a reference test to compare the results of the ELISA and Western blotting (WB); the specificity and sensitivity of the VP2332-452ELISA were 97.9% and 97.3%, respectively, which were higher than those of WB. Therefore, this VP2332-452ELISA may be a preferable method for detecting antibodies against AMDV.

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