Evaluation of Enzyme -Linked Immunosorbent Assay (ELISA) and Western Blotting for the immunodiagnosis of hydatid diseases in Sheep and Goats

10.5580/86c ◽  
2009 ◽  
Vol 5 (2) ◽  
Keyword(s):  
Western Blotting ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sheep And Goats

A Comparison of an Enzyme Linked Immunosorbent Assay (ELISA) and Western Blotting for Detection of Peanut Mottle Virus and Peanut Stripe Virus1

1986 ◽  
Vol 13 (2) ◽  
pp. 64-67 ◽  
Author(s):  
J. L. Sherwood ◽  
H. A. Melouk
Keyword(s):  
Western Blotting ◽  
Immunosorbent Assay ◽  
Protein A ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Coat Proteins ◽  
Western Blots ◽  
Peanut Stripe Virus ◽  
Dry Milk ◽  
The Difference

Abstract Western blotting was used to detect infections of peanut cv. Tamnut 74 with peanut mottle virus (PMV) and/or peanut stripe virus (PStV). Leaf samples were ground in electrophoresis sample buffer and heated for 5 min at 95 C prior to electrophoresis in 12% polyacrylamide gels. After electrophoresis, proteins were transferred to nitrocellulose sheets at 100V for 45 min. Western blots were performed by first blocking unbound sites on the nitrocellulose with 5% non-fat dry milk in Tris-buffered saline (TBS), pH 7.4 for 30 min, followed by incubation in a 1/200 dilution of PMV and/or PStV antiserum in TBS (the latter antiserum provided by J. W. Demski, U. of GA) for 45 min. This was followed by incubation in protein-A-peroxidase (2 μg/mL in TBS) for 45 min, followed by 4-chloro-1-napthol plus hydrogen peroxide in TBS. As little as 25 ng of either purified PMV or PStV was detected. This was similar to the limits of detection fo the double sandwich enzyme linked immunosorbent assay (ELISA). Because of the difference in migration of the coat proteins of PMV and PStV, both viruses may be detected in plants infected with PMV and PStV. This assay can be performed in approximately 6 h when mini-gels are used for the initial electrophoretic seperation and does not require the antiserum to be fractionated or bound to an enzyme as is the case with ELISA.

scholarly journals Development of an Enzyme-Linked Immunosorbent Assay Based on Fusion VP2332-452Antigen for Detecting Antibodies against Aleutian Mink Disease Virus

2015 ◽  
Vol 54 (2) ◽  
pp. 439-442 ◽  
Author(s):  
Xiaowei Chen ◽  
Cailing Song ◽  
Yun Liu ◽  
Liandong Qu ◽  
Dafei Liu ◽  
...  
Keyword(s):  
Disease Virus ◽  
Western Blotting ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Reference Test ◽  
Aleutian Mink Disease Virus ◽  
Specificity And Sensitivity

For detection of Aleutian mink disease virus (AMDV) antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed using the recombinant VP2332-452protein as an antigen. Counterimmunoelectrophoresis (CIEP) was used as a reference test to compare the results of the ELISA and Western blotting (WB); the specificity and sensitivity of the VP2332-452ELISA were 97.9% and 97.3%, respectively, which were higher than those of WB. Therefore, this VP2332-452ELISA may be a preferable method for detecting antibodies against AMDV.

scholarly journals Seroconversion to Brucella spp. and Toxoplasma gondii in Sheep and Goats in Dohuk Province, Iraq and Its Association with Pregnancy Loss

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 836
Author(s):  
Ali Al Hamada ◽  
Ihab Habib ◽  
Mieghan Bruce ◽  
Anne Barnes ◽  
Ian D. Robertson
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Ultrasound Examination ◽  
Small Ruminants ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sheep And Goats ◽  
Northern Iraq ◽  
Incidence Risk ◽  
In Series ◽  
The Impact

In this study, sera from 240 small ruminants (192 sheep and 48 goats) belonging to 12 farms in Dohuk Province, northern Iraq, were collected on two occasions to investigate the incidence risk of seroconversion to Brucella spp. and Toxoplasma gondii. All selected animals were confirmed pregnant (approximately 2 months pregnant) by ultrasound examination at the time of the first blood collection. A second ultrasound examination and blood sampling were undertaken two months after the initial scanning/sampling. Antibodies to Brucella were tested using the Rose Bengal Test (RBT) and an indirect enzyme-linked immunosorbent assay (iELISA), and the results were interpreted in series. The Latex Agglutination Test (LAT) and an indirect enzyme-linked immunosorbent assay (iELISA) were also used in series to confirm the presence of antibodies to T. gondii. The seroprevalence for Brucella and Toxoplasma increased significantly between the two sampling times (p = 0.0003 and 0.03 in first and second sampling, respectively). The incidence risk of seroconversion to Brucella over the two months was 10.6% (95% CI: 6.9–15.3) and 7.3% (95% CI: 4.3–11.6) for Toxoplasma. Animals that seroconverted to Brucella were 2.9 times more likely to lose their pregnancy (95% CI: 1.6–5.5) than animals that remained seronegative; however, seroconversion to Toxoplasma had no significant impact on loss of pregnancy. This study is the first reported investigation on the association of seroconversion to Brucella and Toxoplasma with the reproductive outcome of pregnant sheep and goats in northern Iraq. Brucellosis and toxoplasmosis continue to negatively impact small ruminants' reproductive performance and compromising food security in Iraq. It is hoped that this study will assist the development of a better-informed economic model to estimate Brucella and Toxoplasma burden in small animals in northern Iraq, and such a model could be used to validate the impact of various potential intervention programs in.

scholarly journals Serological investigation of ovine chlamydiosis in small ruminants in Western Turkey

2020 ◽  
Vol 89 (3) ◽  
pp. 255-261
Author(s):  
Mehmet Engin Malal ◽  
Mustafa Sencer Karagül ◽  
Kadir Akar
Keyword(s):  
Immunosorbent Assay ◽  
Small Ruminants ◽  
Enzyme Linked Immunosorbent Assay ◽  
Marmara Region ◽  
Western Turkey ◽  
Surveillance Studies ◽  
Chlamydia Abortus ◽  
Sheep And Goats ◽  
Seroprevalence Data ◽  
Financial Losses

The aim of this study was to investigate the seroprevalence of ovine chlamydiosis caused by Chlamydia abortus in sheep and goats in Western Turkey. Chlamydial abortion causes late term abortions with a worldwide occurence particularly in sheep and goats; it also leads to significant financial losses. Seroepidemiological studies provide useful data regarding the prevalence of the disease. Isolation of Chlamydia abortus as the causative agent of the disease is a time consuming and laborious procedure requiring appropriate biosafety measures. Serological methods are commonly used for routine diagnosis and enzyme-linked immunosorbent assay is generally recommended for surveillance studies. In this study, a total of 833 blood samples obtained from 126 herds of sheep and goats located in all provinces of the Marmara region, Western Turkey, were analyzed. Total seroprevalence was found to be 25.81% through enzyme-linked immunosorbent assay. However, the proportion of seropositive herds was observed at 62.70%, which is higher than the total seroprevalence. This study confirms the presence of Chlamydia abortus exposure in sheep and goat herds in the Marmara region and provides original seroprevalence data in the provinces, which have not been reported so far. The data gathered are useful for the evaluation and elaboration on the seroprevalence of chlamydiosis in small ruminants in the Marmara region, Turkey.

Sign in / Sign up

Export Citation Format

Share Document