scholarly journals In Vitro Cultivation of the Third and Fourth Stage Larvae of Angiostrongylus cantonensis.

1993 ◽  
Vol 55 (2) ◽  
pp. 345-347 ◽  
Author(s):  
Hidekazu HATA
PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e72084 ◽  
Author(s):  
Rong-Jyh Lin ◽  
Jie-Wen He ◽  
Li-Yu Chung ◽  
June-Der Lee ◽  
Jiun-Jye Wang ◽  
...  

2014 ◽  
Vol 113 (5) ◽  
pp. 1883-1896 ◽  
Author(s):  
Zhengyu Li ◽  
Xiaoguang Chen ◽  
Xin Zen ◽  
Jinyi Liang ◽  
Jie Wei ◽  
...  

2009 ◽  
Vol 163 (4) ◽  
pp. 348-356 ◽  
Author(s):  
Emanuele Brianti ◽  
Salvatore Giannetto ◽  
Donato Traversa ◽  
Sharon R. Chirgwin ◽  
Krishna Shakya ◽  
...  

1983 ◽  
Vol 29 (11) ◽  
pp. 1589-1591
Author(s):  
R. Turcotte ◽  
M. Ishaque

Mycobacterium lepraemurium was cultivated in vitro on Ogawa egg-yolk medium. The pathogenicity of the third and eighth subcultures for C3H and C57BL mice was compared with that of in vivo grown murine bacilli by evaluating the mean survival time of infected mice. The results strongly suggest that a significant drop of virulence occurs during the in vitro cultivation of M. lepraemurium.


1983 ◽  
Vol 57 (4) ◽  
pp. 319-324 ◽  
Author(s):  
J. B. Lok ◽  
M. Mika-Grieve ◽  
R. B. Grieve

AbstractMicrofilariae of Dirofilaria immitis retained their infectivity for susceptible mosquitoes after cooling to −196°C in the presence of 5% dimethylsulphoxide (Me2SO) using a two-step cooling sequence. Motility and in vitro development of cryopreserved microfilariae also compared favourably with unfrozen controls. Third-stage larvae frozen by the same cooling sequence in the presence of either 5% Me2SO or 16% hydroxyethyl starch were motile upon thawing. Thawed larvae completed the third- to fourth-stage moult in vitro at a frequency approximately 5 to 10% of that seen in unfrozen controls.


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