Detection of Rabies Virus RNA Isolated from Several Species of Animals in Brazil by RT-PCR.

This report describes four suspected rabies cases in domestic dogs that were involved inhuman exposures. In all these cases, the animals were buried for substantial times beforerabies testing was performed. Animal rabies is endemic in South Africa and domestic dogsare the main vector for transmission to humans. Diagnosis of rabies in humans is complicated,and diagnosis in the animal vector can provide circumstantial evidence to support clinicaldiagnosis of rabies in humans. The gold standard diagnostic method, fluorescent antibodytest (FAT), only delivers reliable results when performed on fresh brain material and thereforedecomposed samples are rarely submitted for diagnostic testing. Severely decomposed brainmaterial was tested for the presence of rabies virus genomic material using a quantitativereal-time reverse transcription polymerase chain reaction (q-real-time RT-PCR) assaywhen conventional molecular methods were unsuccessful. This may be a useful tool in theinvestigation of cases where the opportunity to sample the suspected animals post mortem wasforfeited and which would not be possible with conventional testing methodologies becauseof the decomposition of the material.

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Performance characteristics of a real-time RT-PCR assay for quantification of hepatitis C virus RNA in patients with genotype 1 and 2 infections

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.2008.082 ◽

2008 ◽

Vol 46 (4) ◽

Cited By ~ 2

Author(s):

Jee-Fu Huang ◽

Chia-Yen Dai ◽

Ya-Yun Lin ◽

Ming-Lung Yu ◽

Shu-Fen Liu ◽

...

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Real Time ◽

Performance Characteristics ◽

Genotype 1 ◽

Rt Pcr ◽

Hepatitis C Virus Rna ◽

Pcr Assay ◽

Virus Rna

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Detection of Schmallenberg Virus RNA in Bull Semen in Poland

Polish Journal of Veterinary Sciences ◽

10.1515/pjvs-2016-0083 ◽

2016 ◽

Vol 19 (3) ◽

pp. 655-657 ◽

Cited By ~ 6

Author(s):

J. Kęsik-Maliszewska ◽

M. Larska

Keyword(s):

Extraction Method ◽

Rna Extraction ◽

Viral Rna ◽

Schmallenberg Virus ◽

Rt Pcr ◽

Bovine Semen ◽

Bull Semen ◽

Virus Rna ◽

Virus Excretion ◽

Venereal Transmission

Abstract The detection of Schmallenberg virus (SBV) in the breeding bull semen raised the question of the possibility of venereal transmission of SBV which could result in cost-intensive restrictions in the trade of bovine semen. In order to evaluate the presence of SBV RNA in bovine semen, 131 bull semen samples from four locations in Poland collected between 2013 and 2015 were analysed by RT-PCR for viral RNA. SBV RNA was detected in 5.3% of the samples. The study has revealed that application of an appropriate RNA extraction method is crucial to detect virus excretion via semen.

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Rapid and quantitative detection of mumps virus RNA by one-step real-time RT-PCR

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2004.02.007 ◽

2004 ◽

Vol 49 (2) ◽

pp. 83-88 ◽

Cited By ~ 10

Author(s):

Ayhan Kubar ◽

Mehmet Yapar ◽

Bulent Besirbellioglu ◽

I.Yasar Avci ◽

Cakır Guney

Keyword(s):

Real Time ◽

Mumps Virus ◽

Quantitative Detection ◽

Rt Pcr ◽

Virus Rna ◽

One Step

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A new dual-targeting real-time RT-PCR assay for hepatitis D virus RNA detection

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2018.05.016 ◽

2018 ◽

Vol 92 (2) ◽

pp. 112-117 ◽

Cited By ~ 1

Author(s):

Yan Wang ◽

Jeffrey S. Glenn ◽

Mark A. Winters ◽

Li-ping Shen ◽

Ingrid Choong ◽

...

Keyword(s):

Real Time ◽

Rt Pcr ◽

Pcr Assay ◽

Rna Detection ◽

Hepatitis D ◽

Hepatitis D Virus ◽

Dual Targeting ◽

Virus Rna

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Cocirculation of two dengue virus serotypes in individual and pooled samples of Aedes aegypti and Aedes albopictus larvae

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86822011000100023 ◽

2011 ◽

Vol 44 (1) ◽

pp. 103-105 ◽

Cited By ~ 20

Author(s):

José Eduardo Marques Pessanha ◽

Waleska Teixeira Caiaffa ◽

Alzira Batista Cecilio ◽

Felipe Campos de Melo Iani ◽

Simone Costa Araujo ◽

...

Keyword(s):

Aedes Aegypti ◽

Dengue Virus ◽

Clinical Presentation ◽

Rt Pcr ◽

Virus Rna ◽

Dengue Virus Serotypes ◽

Belo Horizonte ◽

The City ◽

Pooled Samples ◽

Virological Surveillance

INTRODUCTION: To detect dengue virus, eggs of Aedes sp were collected in the city of Belo Horizonte, Brazil, in 2007. METHODS: Egg samples were subsequently hatched and the larvae were tested for the presence of dengue virus RNA by RT-PCR. RESULTS: Among the Aedes aegypti larvae samples, 163 (37.4%) out of 435 were positive, including 32 (10.9%) of 293 individual larvae samples concomitantly positive for two serotypes. CONCLUSIONS: Virological surveillance detecting coinfected vectors in the field could represent an important strategy for understanding the numerous factors involved in the transmission and clinical presentation of dengue.

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