Dynamic Changes in Plasma Concentrations of Gonadotropins, Inhibin, Estradiol-17.BETA. and Progesterone in Cows with Ultrasound-guided Follicular Aspiration.

The aim of this study was to evaluate the applicability of ovum pick-up equipment for follicular fluid collection from various follicular structures (experiment 1) and for recovery of follicular fluid for acid-base balance analysis (experiment 2). An ultrasound scanner equipped with a 5-MHz convex transducer was used for transvaginal ultrasound-guided follicular aspiration. A 17-gauge, 60-cm aspiration needle was connected with a shortened aspiration line. The fluid was aspirated manually into a 2 ml plastic syringe at a speed of approximately 0.2 ml/s. The success of aspiration was higher in ovarian cysts (100%) and single follicles larger than 13 mm (76.7%) compared to single follicles smaller than 12 mm (20%,p< 0.001). The success of aspiration of multiple follicles on day 4 (diameter of 7–9 mm) was higher (90.9%) compared to follicles on day 2 (diameter of 4-6 mm) (66.7%,p< 0.05) in experiment 1. The fluid from ovarian cysts > 25 mm in diameter was aspirated in a two-step procedure (samples 1 and 2) for the determination of pH, HCO3,BE, pCO2and pO2(experiment 2). The indicators were compared between samples 1 and 2. Higher pO2as well as pH and lower pCO2in sample 1 compared to sample 2 showed insufficient anaerobic conditions during the first phase of the puncture in experiment 2. Our study brings for the first time the finding that the ovum pick-up equipment used in our experiments is suitable for the collection of follicular fluid only from larger follicular structures. The sampling of follicular fluid for acid-base balance assays requires the development of a special new device to prevent samples from coming into contact with air during aspiration.

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Transvaginal ultrasound-guided follicular aspiration of equine oocytes

Equine Veterinary Journal ◽

10.1111/j.2042-3306.1993.tb04831.x ◽

2010 ◽

Vol 25 (S15) ◽

pp. 71-74 ◽

Cited By ~ 2

Author(s):

N. L. COOK ◽

E. L. SQUIRES ◽

B. S. RAY ◽

D. J. JASKO

Keyword(s):

Transvaginal Ultrasound ◽

Ultrasound Guided ◽

Follicular Aspiration

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Transabdominal Ultrasound-guided Follicular Aspiration in Case of Müllerian Anomaly with Ovarian Malposition

Journal of SAFOG with DVD ◽

10.5005/jp-journals-10006-1361 ◽

2015 ◽

Vol 7 (3) ◽

pp. 213-215

Author(s):

Vineet V Mishra ◽

Preeti A Goyal

Keyword(s):

Oocyte Retrieval ◽

Controlled Ovarian Hyperstimulation ◽

Fertilization Rate ◽

Transabdominal Ultrasound ◽

Ultrasound Guided ◽

Vitro Fertilization ◽

Follicular Aspiration ◽

Mullerian Anomaly ◽

Embryo Number

ABSTRACT Objective To describe the approach of transabdominal ultrasound-guided oocyte retrieval in a patient of müllerian anomaly and ovarian malposition. Setting A tertiary referral reproductive medicine unit. Patient A 23-year-old patient with müllerian anomaly (right unicornuate with left rudimentary horn uterus) and ovarian malposition. Intervention Controlled ovarian hyperstimulation, transabdominal ultrasound-guided oocyte retrieval. Main outcome measure Total and mature oocytes retrieved, fertilization rate, embryo number and quality. Result Five oocytes were retrieved transabdominally using percutaneous needle puncture under ultrasound guidance. Conclusion In in vitro fertilization (IVF) protocols, patients with müllerian anomaly with inaccessible ovaries vaginally, transabdominal ultrasound-guided follicular aspiration is a safe and efficacious alternative technique for oocyte retrieval. How to cite this article Mishra VV, Choudhary S, Goyal PA, Aggarwal R. Transabdominal Ultrasound-guided Follicular Aspiration in Case of Müllerian Anomaly with Ovarian Malposition. J South Asian Feder Obst Gynae 2015;7(3):213-215.

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In vivo ultrasound guided transvaginal oocyte collection in the cow

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.15773 ◽

2018 ◽

Vol 49 (3) ◽

pp. 195

Author(s):

G. S. AMIRIDIS (Γ.Σ. ΑΜΟΙΡΙΔΗΣ) ◽

M. SALAHEDDINE ◽

I. A. JEFFCOATE ◽

E. VAINAS (Ε. ΒΑΪΝΑΣ) ◽

L. ROBERTSON

Keyword(s):

Recovery Rate ◽

Transvaginal Ultrasound ◽

Oestrous Cycle ◽

Ultrasound Guided ◽

Follicular Aspiration ◽

Oocyte Recovery ◽

Oocyte Collection ◽

Significant Difference

This paper describes the results of the in vivo ultrasound guided follicular aspiration for ovum pick υρ (OPU) in the cow. Twelve non pregnant dry cows aged 4-6 years were used in this experiment. Eight cows underwent OPU during three successive oestrous cycles and another four cows were used as controls having only transvaginal ultrasound scanning of their ovaries. Oocyte collection took place three times during the luteal phase of each natural oestrous cycle (days 3-4,9-11 and 14-17). The content of 326 follicles with a diameter of 4-15mm was aspirated and 104 oocytes were collected (recovery rate 31.9% or 1.55 oocytes per cow and session). The oocyte recovery rate increased after the first three sessions (from 13.04% to 35.0%) and reached levels of υρ to 52.6%. More follicles were aspirated on days 9-11 (133 follicles 40.8%) compared to 111 (34%) follicles on days 14-17 and 82 (25%) on days 3-4) (P<0.05). The evaluation of the collected oocytes revealed that 60 oocytes (57.7%) were suitable for further in vitro manipulation. Neither the origin of the oocyte (left or right ovary) nor the stage of the oestrous cycle affected the recovery rate or the quality of the collected oocytes. There was no significant difference either in the length of the oestrous cycle between the experimental animals and the controls (21.6± 1.4 vs. 22.37±1.0 respectively), or in plasma progesterone concentration in daily collected blood samples from the animals of the two groups. The results of this study are compared to those from the international literature and to the results from endoscopical methods for oocyte recovery. The feasibility of application of this technique to projects designed to improve the genetic merit of cows is discussed.

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Technical aspects of transvaginal ultrasound-guided follicular aspiration in cows

Veterinary Record ◽

10.1136/vr.134.17.440 ◽

1994 ◽

Vol 134 (17) ◽

pp. 440-443 ◽

Cited By ~ 14

Author(s):

C. Scott ◽

L. Robertson ◽

R. de Moura ◽

C. Paterson ◽

J. Boyd

Keyword(s):

Transvaginal Ultrasound ◽

Ultrasound Guided ◽

Technical Aspects ◽

Follicular Aspiration

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In vitro embryo production from bovine oocyte donors aspirated at different frequencies or following treatment with FSH

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200592680 ◽

1996 ◽

Vol 1996 ◽

pp. 68-68

Author(s):

K.L. Goodhand ◽

R.G. Watt ◽

M.E. Staines ◽

L.C. Higgins ◽

P.J. Broadbent ◽

...

Keyword(s):

Transvaginal Ultrasound ◽

Genetic Change ◽

Bovine Oocyte ◽

Ultrasound Guided ◽

Embryo Production ◽

Follicular Aspiration ◽

Oocyte Donors ◽

Pre Treatment

The combination of in vivo recovery of oocytes using transvaginal ultrasound guided aspiration and subsequent in vitro embryo production can be used to increase the rate of genetic change for efficiency of beef production by increasing selection intensity and reducing generation interval. The total number of oocytes recovered by aspiration and embryos produced is directly proportional to the number of aspiration sessions whether recovery takes place once or twice weekly. Pre-treatment of oocyte donors with FSH has been shown to improve the number of follicles available for aspiration but effects on embryo production have been conflicting (Bungartz et al., 1995; Goodhand et al., in press). The objective of this experiment was to compare the effect on embryo production of frequency of follicular aspiration and pre-treatment of donor cattle with FSH.

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