Theoretical Prediction of Stress Fiber Orientation in Cultured Cells under Uniaxial Cyclic Stretch

2000 ◽  
Vol 2000.2 (0) ◽  
pp. 255-256
Author(s):  
Hiroshi YAMADA ◽  
Tohru TAKEMASA ◽  
Takami YAMAGUCHI
Keyword(s):  
Theoretical Prediction ◽  
Fiber Orientation ◽  
Cultured Cells ◽  
Stress Fiber ◽  
Cyclic Stretch

The Direction of Cyclic Stretch-Induced Stress Fiber Orientation Depends on Matrix Rigidity

2011 ◽  
Author(s):  
Abhishek Tondon ◽  
Hui-Ju Hsu ◽  
Roland Kaunas
Keyword(s):  
Theoretical Model ◽  
Fiber Orientation ◽  
Stress Fiber ◽  
Cyclic Stretch ◽  
Matrix Rigidity ◽  
Structural Element ◽  
Cellular Processes ◽  
Induced Stress ◽  
Cellular Environment ◽  
Collagen Hydrogels

Mechanical properties of the cellular environment such as elastic rigidity have been shown to play an important role in the regulation of important cellular processes such as proliferation, differentiation and apoptosis (1–3). Intracellular tension decreases with decreasing matrix rigidity (1). Actin stress fibers (SFs), the major structural element in cells bearing tension, are also less prevalent on soft vs. rigid matrices (4). We have developed a theoretical model of stretch-induced SFs that predicts SFs reorient perpendicular to the direction of cyclic stretch in order to maintain SF tension at a homeostatic level (5). A theoretical model developed by the Safran group (6) predicts that cells will also align perpendicular to cyclic stretch on soft substrates. To test these predictions, we subjected cells to cyclic uniaxial stretch on soft collagen hydrogels. Interestingly, the cells and their SFs aligned parallel to the direction of stretch without co-alignment of collagen fibrils, indicating the need for a new model to describe the effects of cyclic stretch on SF reorganization on soft matrices.

On the Intracellullar Memory of Stress Fiber Orientation: Effects of Microtubules and Focal Adhesions on the Repolymerization Process of Stress Fibers

2008 ◽  
Author(s):  
Yunfeng Yang ◽  
Kazuaki Nagayama ◽  
Takeo Matsumoto
Keyword(s):  
Fiber Orientation ◽  
Cell Movement ◽  
Focal Adhesions ◽  
Stress Fiber ◽  
Cyclic Stretch ◽  
Stress Fibers ◽  
Cellular Functions ◽  
Division 1 ◽  
Key Features ◽  
Strain Magnitude

Stress fibers (SFs) play essential roles in various cellular functions such as cell movement, shape maintenance and cell division [1]. One of their key features is that they dynamically change their structures in response to mechanical environment to which they are exposed [2]. For example, cultured endothelial cells exposed to cyclic stretch preferentially reorganize their actin stress fibers to the direction in which the strain magnitude of the fibers become minimum [3].

Mechanically Optimized Orientation of Intracellular Stress Fibers Under Cyclic Stretch

2000 ◽  
Author(s):  
Hiroshi Yamada ◽  
Tohru Takemasa ◽  
Takami Yamaguchi
Keyword(s):  
Endothelial Cell ◽  
Continuum Mechanics ◽  
Cellular Response ◽  
Length Change ◽  
Mechanical Stimulus ◽  
Stress Fiber ◽  
Cyclic Stretch ◽  
Stress Fibers ◽  
Biological Phenomenon ◽  
The Continuum

Abstract To elucidate the orientation of stress fibers in a cultured endothelial cell under cyclic stretch, we hypothesized that a stress fiber aligns so as to minimize the summation of its length change under cyclic stretch, and that there is a limit in the sensitivity of cellular response to the mechanical stimulus. Results from numerical simulations based on the continuum mechanics describe the experimental observations under uniaxial stretch well. They give us an insight to the biological phenomenon of the orientation in stress fibers under biaxial stretch from the viewpoint of mechanical engineering.

scholarly journals Gaussian Curvature Directs Stress Fiber Orientation and Cell Migration

2018 ◽  
Vol 114 (6) ◽  
pp. 1467-1476 ◽  
Author(s):  
Nathan D. Bade ◽  
Tina Xu ◽  
Randall D. Kamien ◽  
Richard K. Assoian ◽  
Kathleen J. Stebe
Keyword(s):  
Cell Migration ◽  
Gaussian Curvature ◽  
Fiber Orientation ◽  
Stress Fiber

scholarly journals Effect of Cyclic Stretch on Neuron Reorientation and Axon Outgrowth

2020 ◽  
Vol 8 ◽  
Author(s):  
Ji Lin ◽  
Xiaokeng Li ◽  
Jun Yin ◽  
Jin Qian
Keyword(s):  
Mechanical Response ◽  
Focal Adhesions ◽  
Theoretical Work ◽  
Stress Fiber ◽  
Cyclic Stretch ◽  
Axon Outgrowth ◽  
Amplitude Dependence ◽  
Reference Case ◽  
Axon Elongation ◽  
As Stress

The directional alignment and outgrowth of neurons is a critical step of nerve regeneration and functional recovery of nerve systems, where neurons are exposed to a complex mechanical environment with subcellular structures such as stress fibers and focal adhesions acting as the key mechanical transducer. In this paper, we investigate the effects of cyclic stretch on neuron reorientation and axon outgrowth with a feasible stretching device that controls stretching amplitude and frequency. Statistical results indicate an evident frequency and amplitude dependence of neuron reorientation, that is, neurons tend to align away from stretch direction when stretching amplitude and frequency are large enough. On the other hand, axon elongation under cyclic stretch is very close to the reference case where neurons are not stretched. A mechanochemical framework is proposed by connecting the evolution of cellular configuration to the microscopic dynamics of subcellular structures, including stress fiber, focal adhesion, and microtubule, yielding theoretical predictions that are consistent with the experimental observations. The theoretical work provides an explanation of the neuron’s mechanical response to cyclic stretch, suggesting that the contraction force generated by stress fiber plays an essential role in both neuron reorientation and axon elongation. This combined experimental and theoretical study on stretch-induced neuron reorientation may have potential applications in neurodevelopment and neuron regeneration.

Sign in / Sign up

Export Citation Format

Share Document