C214 Effects of Ultrasonic Irradiation on Ice Formation in Biological Tissue

2007 ◽  
Vol 2007 (0) ◽  
pp. 251-252
Author(s):  
Yukio TADA ◽  
Akira TAKIMOTO ◽  
Haruki TUKAMOTO ◽  
Hajime ONISHI
Keyword(s):  
Ultrasonic Irradiation ◽  
Biological Tissue ◽  
Ice Formation

Effects of Ultrasonic Irradiation on Ice Formation in Biological Tissue

2010 ◽  
Author(s):  
Yukio Tada ◽  
Yoshiaki Satou ◽  
Makoto Kurokawa ◽  
Akira Takimoto ◽  
Hajime Onishi
Keyword(s):  
Ultrasonic Irradiation ◽  
Biological Tissue ◽  
Ice Formation ◽  
Intensity Level ◽  
Ultrasonic Power ◽  
Promising Technique ◽  
Agar Gel ◽  
Supercooling Degree ◽  
Temperature Solution ◽  
Supercooled Region

A method to actively controlling crystallization is one of promising technique for cryopreservation. The object of this paper is to study the effect of ultrasonic irradiation on ice formation during freezing of biological tissue with supercooling. In the experiments, agar gel was frozen under irradiation of ultrasound at frequency of 28kHz. The measurements of temperature and the microscopic observation of ice crystals using fluorescent indicator were carried out. Firstly, it was found that the supercooled state was released by applying ultrasound at high intensity level, since ultrasonic cavitation effects were augmented. On the other hand, a weakly delay in the nucleation temperature was induced by applying ultrasound at low intensity level. Secondly, ice structure size decreased with increasing ultrasonic power under given supercooling degree at nucleation stage. The effect of ultrasound was enhanced by using large supercooling condition. Thirdly, paying attention to the thermal effect of ultrasound as changing temperature profile, sound and heat conduction in agar gel immersed in low temperature solution were numerically calculated. It was suggested that ultrasound at high frequency is effective to enlarge supercooled region.

scholarly journals A151 Ice Formation under Ultrasonic Irradiation

2005 ◽  
Vol 2005 (0) ◽  
pp. 29-30
Author(s):  
Yukio TADA ◽  
Akira TAKIMOTO ◽  
Tubasa KONYA ◽  
Yujiro HAYASHI
Keyword(s):  
Ultrasonic Irradiation ◽  
Ice Formation

Advances in Microprobe Analysis Applied to Muscle, Nerve and Gland

1982 ◽  
Vol 40 ◽  
pp. 404-407
Author(s):  
T. E. Hutchinson ◽  
D. E. Johnson ◽  
A. C. Lee ◽  
E. Y. Wang
Keyword(s):  
Biological Tissue ◽  
Microprobe Analysis ◽  
Physiological State ◽  
External Environment ◽  
Physiological Relevance ◽  
Muscle Nerve ◽  
Technique Development

Microprobe analysis of biological tissue is now in the end phase of transition from instrumental and technique development to applications pertinent to questions of physiological relevance. The promise,implicit in early investigative efforts, is being fulfilled to an extent much greater than many had predicted. It would thus seem appropriate to briefly report studies exemplifying this, ∿. In general, the distributions of ions in tissue in a preselected physiological state produced by variations in the external environment is of importance in elucidating the mechanisms of exchange and regulation of these ions.

Complementary Z-Contrast Imaging and Digital Image Processing

1985 ◽  
Vol 43 ◽  
pp. 304-305
Author(s):  
K. N. Colonna ◽  
G. Oliphant
Keyword(s):  
Image Processing ◽  
Heavy Metal ◽  
Digital Image Processing ◽  
Digital Image ◽  
Biological Tissue ◽  
Biological Imaging ◽  
Tissue Preparation ◽  
Contrast Imaging ◽  
Imaging Tool ◽  
Z Contrast

Harmonious use of Z-contrast imaging and digital image processing as an analytical imaging tool was developed and demonstrated in studying the elemental constitution of human and maturing rabbit spermatozoa. Due to its analog origin (Fig. 1), the Z-contrast image offers information unique to the science of biological imaging. Despite the information and distinct advantages it offers, the potential of Z-contrast imaging is extremely limited without the application of techniques of digital image processing. For the first time in biological imaging, this study demonstrates the tremendous potential involved in the complementary use of Z-contrast imaging and digital image processing.Imaging in the Z-contrast mode is powerful for three distinct reasons, the first of which involves tissue preparation. It affords biologists the opportunity to visualize biological tissue without the use of heavy metal fixatives and stains. For years biologists have used heavy metal components to compensate for the limited electron scattering properties of biological tissue.

PHOTOPROCESSING OF METHANOL ICE: FORMATION AND LIBERATION OF CO

2017 ◽  
Author(s):  
Houston Smith ◽  
Susanna Widicus Weaver ◽  
Stefanie Milam ◽  
Samuel Zinga ◽  
AJ Mesko
Keyword(s):  
Ice Formation

Eco-Friendly Synthesis of Pyrazoline Derivatives

2017 ◽  
Vol 9 (04) ◽  
Author(s):  
Mahesh G. Kharatmol ◽  
Deepali Jagdale
Keyword(s):  
Ionic Liquids ◽  
Microwave Irradiation ◽  
Organic Synthesis ◽  
Ultrasonic Irradiation ◽  
Minimal Energy ◽  
Anticancer Activities ◽  
Conventional Methods

Pyrazoline class of compounds serve as better moieties for an array of treatments, they have antibacterial, antifungal, antiinflammatory, antipyretic, diuretic, cardiovascular activities. Apart from these they also have anticancer activities. So, pertaining to its importance, many attempts are made to synthesize pyrazolines. Since conventional methods of organic synthesis are energy and time consuming. There are elaborate pathways for green and eco-friendly synthesis of pyrazoline derivatives including microwave irradiation, ultrasonic irradiation, grinding and use of ionic liquids which assures the synthesis of the same within much lesser time and by use of minimal energy

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