Technique development of high-throughput and high-sensitivity sample preparation and separation for proteomics

Sample preparation and separation methods determine the sensitivity and the quantification accuracy of the proteomics analysis. This article covers a comprehensive review of the recent technique development of high-throughput and high-sensitivity sample preparation and separation methods in proteomics research.

RP-HPLC QUANTIFIABLE TECHNIQUE DEVELOPMENT FOR EVALUATING PREGABALIN AND ETORICOXIB COMBINATION IN TABLET AND BULK KINDS

Objective: This evaluation study aims to initiate a relatively sensitive RP-HPLC quantifiable technique for evaluating pregabalin (PRBN) and etoricoxib (ETRB) combination in tablet and bulk kinds. Methods: PRBN and ETRB chromatographic evaluations were carried off using the “KNAUER C18 Eurospher II column (250 mm × 4.6 mm × 5 μ)”. The mobile phase (MBP) was driven into the KNAUER C18 Eurospher II column at a 1.0 ml/min run rate with an isocratic elution programme of 65% volume of 0.5 mmol sodium perchlorate 35% volume methanol, detected and evaluated the PRBN and ETRB content at 217 nm. Results: The analysis of PRBN and ETRB is executed inside a run period of 15 min. The RP-HPLC quantifiable technique was developed to separate PRBN and ETRB and likely degradants formed from stress testing by isocratic elution. The RP-HPLC quantifiable technique developed was successfully validated to existing ICH limit guidelines and was confirmed as robust, specific, accurate, selective, precise, sensitive, and linear. Conclusion: The RP-HPLC quantifiable technique developed here is more valuable and worthy for routine PRBN and ETRB analysis of tablets and bulk kinds.

Atomic-precision advanced manufacturing for Si quantum computing

A materials synthesis method that we call atomic-precision advanced manufacturing (APAM), which is the only known route to tailor silicon nanoelectronics with full 3D atomic precision, is making an impact as a powerful prototyping tool for quantum computing. Quantum computing schemes using atomic (31P) spin qubits are compelling for future scale-up owing to long dephasing times, one- and two-qubit gates nearing high-fidelity thresholds for fault-tolerant quantum error correction, and emerging routes to manufacturing via proven Si foundry techniques. Multiqubit devices are challenging to fabricate by conventional means owing to tight interqubit pitches forced by short-range spin interactions, and APAM offers the required (Å-scale) precision to systematically investigate solutions. However, applying APAM to fabricate circuitry with increasing numbers of qubits will require significant technique development. Here, we provide a tutorial on APAM techniques and materials and highlight its impacts in quantum computing research. Finally, we describe challenges on the path to multiqubit architectures and opportunities for APAM technique development. Graphic Abstract

THE TECHNIQUE OF PSYCHOSEMANTIC ANALYSIS OF STUDENTS’ ATTITUDES TO SELF-EDUCATIONAL ACTIVITIES

The article presents the author’s technique analysing psychosemantically students’ attitudes to self-educational activities, which can be used in psychological and pedagogical practice both for research purposes and for psychological and pedagogical assistance for students by developing their readiness for self-education. It is substantiated that the study of the constructs of students’ consciousness, which reflect their attitudes to different self-educational activities, using the method of semantic differential, makes it possible to identify: how difficult to implement self-educational activities (including specific activities, such as reading of scientific-professional literature); what self-education means to them; how it affects students; how students understand the importance of such activities for their professional development; how they are interested in these activities and whether they are active in their implementation; to what degree self-education is modern, dynamic; deepness of awareness of what is learned through self-education; the regularity of their self-educational activities; the degree of independence in such activity organization and implementation; effectiveness of self-educational activities. During technique development, twenty-eight scales were identified, which made up the final version of the semantic differential matrix: 1) Fatiguing – Mobilizing, 2) Disciplinary – Relaxing, 3) Effective – Ineffective, 4) Interfering – Helping, 5) With help– Independent, 6) Significant – Unimportant, 7) Externally directed – Self-motivated, 8) Easily accessible – Hard to access, 9) Easy to implement – Difficult to implement, 10) Mass – Individual, 11) Full of meaning – Nonsense, 12) Not worth the effort – Worth the effort, 13) Not worth the attention – Worth the attention, 14) Uninformative – Informative, 15) Necessary – Useless, 16) Unnecessary – Demanded, 17) Unacceptable – Desirable, 18) Unproductive – Effective, 19) New – Traditional, 20) Boring – Interesting, 21) Passively carried out – Actively carried out, 22) Superficial – Deep, 23) Constant – Situational, 24) Regular – Rare, 25) Various – Monotonous, 26) Systematic – Chaotic, 27) Modern – Old-fashioned, 28) Harmful – Important. The expressed differentiating ability of the technique was substantiated. The stages of technique development were described.

History of Piano Technique Development: Methodical Aspect

The history of the development of piano technique has been counting down since the 17th century. The most common keyboard instruments before the invention of the piano were the harpsichord, clavicord and organ. These early instruments had much lighter keys compared to modern ones. Therefore, performers could easily press the keys without feeling much resistance. With the advent of the piano, both the playing technique and the training technique for professional pianists and amateurs began to be reviewed. Musicians, summarizing their own performing experience, wrote treatises, textbooks and manuals, looking for the most effective method of mastering the technique of playing the keyboard instrument. In history, there are two schools of piano technique: the so-called school of “high lifting of fingers” and “playing with all the weight of the hand”. This work is devoted to the history of the development of these schools and methodological approaches to piano training. The views on piano technique that developed in the 18th–20th centuries, which were reflected in the textbooks of pianistsperformers and teachers, help to understand how to perform music of a particular period and what finger technique to use for a perfect interpretation of piano works of the past.

The technique development of non-washing of mineral fertilizers in the soil through the screen formed on the interpolymer complex basis

The problem of global climate change is on the agenda of mankind, not only because of the average annual temperature rise on the planet but also because of changes in the entire geosystem, rising global oceans, melting ice and permanent glaciers, increasing uneven rainfall, changing river flow patterns and climate instability, other changes involved. The world pays special attention to research work to develop technologies for the management and efficient use of water resources. In particular, it is important to conduct research to develop new water-saving irrigation technologies using cheap and chemical, and technical means.