scholarly journals Validated Stability Indicating Liquid Chromatographic Method for Simultaneous Estimation of Dosulepin And Methylcobalamin in Combined Pharmaceutical Dosage Form

2014 ◽  
Vol 30 (3) ◽  
pp. 1243-1251 ◽  
Author(s):  
L Reddy ◽  
S Reddy ◽  
G Reddy
Keyword(s):  
Dosage Form ◽  
Chromatographic Method ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Stability Indicating ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

scholarly journals NOVEL NOVEL STABILITY-INDICATING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY METHOD FOR SIMULTANEOUS ESTIMATION OF HYDROCORTISONE AND TETRACYCLINE IN BULK AND PHARMACEUTICAL DOSAGE FORM

2019 ◽  
pp. 255-258
Author(s):  
MUNI SAI VARAGANTI ◽  
PRASANTHI CHENGALVA ◽  
ARUNA GUNDALA
Keyword(s):  
High Performance ◽  
Dosage Form ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Simultaneous Estimation ◽  
Reverse Phase ◽  
Pharmaceutical Dosage Form ◽  
Stability Indicating ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Objective: A combination of hydrocortisone and tetracycline as topical ophthalmic ointment is used for skin irritations, eye infections, inflammation, skin infections, acne, and rashes. The objective of the current work is to a simple, rapid, accurate, and precise, stability-indicating reverse-phase liquid chromatographic method was developed for the simultaneous estimation of hydrocortisone and tetracycline in bulk and pharmaceutical dosage form. Methods: The separation was carried out in Discovery C18 column (250 × 4.6 mm, 5 μm) using mobile phase ratio of water (pH 2.2 adjusted with orthophosphoric acid):acetonitrile (40:60 v/v) in an isocratic elution mode with a flow rate of 1.0 ml/min at detection wavelength of 244 nm. The injection volume was 10 μl and the column temperature was set at 30°C. Results: The retention time for hydrocortisone and tetracycline was found to be 2.214 ± 0.001 min and 3.497 ± 0.001 min, respectively. Calibration curves were linear (r2=0.999) at a concentration range of 2.5–15 mg/ml for both hydrocortisone and tetracycline. The percentage recoveries were found to be 99.13–99.67% for hydrocortisone and 99.39–99.61% for tetracycline. Relative standard deviation was found to be 0.3% for both the drugs. Limit of detection and limit of quantification values of hydrocortisone and tetracycline were found to be 0.09 μg/ml and 0.27 μg/ml and 0.17 μg/ml and 0.52 μg/ml, respectively. The drugs were subjected to various stress conditions and found no interference of degraded products peak at the retention times of analyte peaks. Conclusion: A rapid and accurate reverse-phase high-performance liquid chromatographic method was developed for simultaneous estimation of hydrocortisone and tetracycline, and the method was validated as per the International Council for Harmonization guidelines. Hence, the developed method can be successfully applied for the simultaneous estimation of hydrocortisone and tetracycline in bulk and ointment formulation.

scholarly journals Stability indicating liquid chromatographic method for the estimation of desvenlafaxine in pharmaceutical dosage form

2011 ◽  
Vol 17 (3) ◽  
pp. 341-348 ◽  
Author(s):  
Dimal Shah ◽  
Riddhi Nakrani ◽  
Sunil Baldania ◽  
Usmangani Chhalotiya ◽  
Kashyap Bhatt
Keyword(s):  
Dosage Form ◽  
Chromatographic Method ◽  
Potassium Dihydrogen Phosphate ◽  
Chemical Oxidation ◽  
Base Hydrolysis ◽  
Dihydrogen Phosphate ◽  
Pharmaceutical Dosage Form ◽  
Stability Indicating ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

A simple, sensitive, precise and stability indicating liquid chromatographic method has been developed for the estimation of desvenlafaxine succinate in pharmaceutical dosage form. A Hypersil C-18, 5 mm column with a mobile phase containing 0.05 M potassium dihydrogen phosphate: methanol (60:40 v/v) pH 7 was used. The flow rate was 1.0 mL/min and effluents were monitored at 226 nm. The retention time of desvenlafaxine was 7.4 min and the method was linear in the range of 0.1-20 ?g/mL. Desvenlafaxine stock solution was subjected to acid and alkali hydrolysis, chemical oxidation and dry heat degradation. The drug was found to be susceptible to base hydrolysis and developed method was found to give good separation between the pure drug and the degraded product. The method was successfully applied to the estimation of desvenlafaxine in tablet dosage forms.

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