Evaluation of a Site Specific Chemical Application System Based on the Spatial Variability of Weeds

2013 ◽  
Author(s):  
F. H. R. Baio and L. A. Balastreire
Keyword(s):  
Spatial Variability ◽  
Chemical Application ◽  
Application System ◽  
Specific Chemical ◽  
Site Specific ◽  
A Site

DEVELOPMENT OF A SITE-SPECIFIC APPLICATION SYSTEM FOR LIQUID ANIMAL MANURES

1999 ◽  
Vol 15 (6) ◽  
pp. 633-638 ◽  
Author(s):  
D. K. Morris ◽  
D. R. Ess ◽  
S. E. Hawkins ◽  
S. D. Parsons
Keyword(s):  
Application System ◽  
Site Specific ◽  
Animal Manures ◽  
A Site

A novel site-specific chemical conjugation of IgG antibodies by affinity peptide for immunoassays

2020 ◽  
Author(s):  
Satoka Mori ◽  
Arisa Abe ◽  
Naoto Ishikawa ◽  
Abdur Rafique ◽  
Yuji Ito
Keyword(s):  
Protein A ◽  
Disease Diagnosis ◽  
Conjugation System ◽  
Specific Chemical ◽  
Site Specific ◽  
Drug Conjugates ◽  
Chemical Conjugation ◽  
Affinity Peptide ◽  
A Site ◽  
Human And Mouse

Abstract Recently, there has been an increasing interest in site-specific modifications of antibodies used in immunoassays for disease diagnosis and as antibody therapeutics, such as antibody−drug conjugates. Previously, we established a site-specific chemical conjugation system using an IgG-Fc binding chemical conjugation affinity peptide (CCAP). CCAP could be used only for the modification of human IgG owing to the lack of affinity of CCAP to rodent IgG molecules. In this study, novel CCAP reagents are proposed, which can be used for both human and mouse IgG, based on the Staphylococcus aureus protein A domain-derived affinity peptides Z34C and Z33. Compared with the activity of a conventional randomly modified antibody, mouse IgG modified using this method had favourable features in two immunoassays, demonstrating the advantages of the proposed CCAP method in preserving antibody functionality during conjugation.

Use of Injection for Site-specific Chemical Application

2015 ◽  
pp. 739-744
Author(s):  
S. J. Nuspl ◽  
W. W. Rudolph ◽  
R. Guthland
Keyword(s):  
Chemical Application ◽  
Specific Chemical ◽  
Site Specific

Reactive Nanopattern in a Triple Structured Bio-Inspired Honeycomb Film as a Clickable Platform

2018 ◽  
Author(s):  
Pierre Marcasuzaa ◽  
Samuel Pearson ◽  
Karell Bosson ◽  
Laurence Pessoni ◽  
Jean-Charles Dupin ◽  
...  
Keyword(s):  
Self Assembly ◽  
Double Porosity ◽  
Surface Groups ◽  
Specific Chemical ◽  
Site Specific ◽  
Surface Functionality ◽  
Wenzel State ◽  
Breath Figure ◽  
Responsive Surfaces ◽  
Secondary Population

A hierarchically structured platform was obtained from spontaneous self-assembly of a poly(styrene)-<i>b</i>-poly(vinylbenzylchloride) (PS-<i>b</i>-PVBC) block copolymer (BCP) during breath figure (BF) templating. The BF process using a water/ethanol atmosphere gave a unique double porosity in which hexagonally arranged micron-sized pores were encircled by a secondary population of smaller, nano-sized pores. A third level of structuration was simultaneously introduced between the pores by directed BCP self-assembly to form out-of-the-plane nano-cylinders, offering very rapid bottom-up access to a film with unprecedented triple structure which could be used as a reactive platform for introducing further surface functionality. The surface nano-domains of VBC were exploited as reactive nano-patterns for site-specific chemical functionalization by firstly substituting the exposed chlorine moiety with azide, then “clicking” an alkyne by copper (I) catalyzed azide-alkyne Huisgen cycloaddition (CuAAC). Successful chemical modification was verified by NMR spectroscopy, FTIR spectroscopy, and XPS, with retention of the micro- and nanostructuration confirmed by SEM and AFM respectively. Protonation of the cyclotriazole surface groups triggered a switch in macroscopic behavior from a Cassie-Baxter state to a Wenzel state, highlighting the possibility of producing responsive surfaces with hierarchical structure.

DECADAL SUMMER LAKE WATER TEMPERATURE VARIABILITY IN SOUTHERN GREENLAND OF THE LAST 2 MILLENNIA: DEVELOPMENT AND APPLICATION OF A SITE-SPECIFIC BRGDGT CALIBRATION

2020 ◽  
Author(s):  
Boyang Zhao ◽  
◽  
Isla S. Castañeda ◽  
Raymond S. Bradley ◽  
Jeff Salacup ◽  
...  
Keyword(s):  
Water Temperature ◽  
Lake Water ◽  
Temperature Variability ◽  
Site Specific ◽  
A Site

scholarly journals Functional expression of a yeast mitochondrial intron-encoded protein requires RNA processing at a conserved dodecamer sequence at the 3' end of the gene.

1989 ◽  
Vol 9 (4) ◽  
pp. 1507-1512 ◽  
Author(s):  
H Zhu ◽  
H Conrad-Webb ◽  
X S Liao ◽  
P S Perlman ◽  
R A Butow
Keyword(s):  
Genetic Analysis ◽  
Rna Processing ◽  
Fold Increase ◽  
Functional Expression ◽  
Rrna Gene ◽  
Yeast Mitochondria ◽  
Wild Type ◽  
Site Specific ◽  
Var1 Gene ◽  
A Site

All mRNAs of yeast mitochondria are processed at their 3' ends within a conserved dodecamer sequence, 5'-AAUAAUAUUCUU-3'. A dominant nuclear suppressor, SUV3-I, was previously isolated because it suppresses a dodecamer deletion at the 3' end of the var1 gene. We have tested the effects of SUV3-1 on a mutant containing two adjacent transversions within a dodecamer at the 3' end of fit1, a gene located within the 1,143-base-pair intron of the 21S rRNA gene, whose product is a site-specific endonuclease required in crosses for the quantitative transmission of that intron to 21S alleles that lack it. The fit1 dodecamer mutations blocked both intron transmission and dodecamer cleavage, neither of which was suppressed by SUV3-1 when present in heterozygous or homozygous configurations. Unexpectedly, we found that SUV3-1 completely blocked cleavage of the wild-type fit1 dodecamer and, in SUV3-1 homozygous crosses, intron conversion. In addition, SUV3-1 resulted in at least a 40-fold increase in the amount of excised intron accumulated. Genetic analysis showed that these phenotypes resulted from the same mutation. We conclude that cleavage of a wild-type dodecamer sequence at the 3' end of the fit1 gene is essential for fit1 expression.

Sign in / Sign up

Export Citation Format

Share Document