scholarly journals Growth of typha grass (Typha angustifolia) on gold-mine tailings with application of arbuscular mycorrhiza fungi

2018 ◽  
Vol 19 (2) ◽  
pp. 454-459 ◽  
Author(s):  
LULUK SETYANINGSIH ◽  
ARUM SEKAR WULANDARI ◽  
HAMIM HAMIM

Setyaningsih L, Wulandari AS, Hamim H. 2018. Growth of typha grass (Typha angustifolia) on gold-mine tailings with application of arbuscular mycorrhiza fungi. Biodiversitas 19: 454-459. Gold mine tailings contain extreme physical and chemical properties, which inhibit plant growth due to lower nutrition and higher heavy metal contaminants. Typha (Typha angustifolia) is type of grass growing well on waterlogged area including tailing dam. The objective of this study was to investigate the effect of arbuscular mycorrhizal fungi (AMF) in combination with compost and soil on the typha growth in gold mine tailings. The study was conducted in greenhouse by inoculating two AMF isolates (Glomus etunicatum and G. manihotis) to typha seedlings grown in pure tailing media, mixed tailing-compost media, and mixed tailing-compost-soil media. The compatibility and growth of typha grass were analysed after 1 month. Results showed that G. etunicatum and G. manihotis application significantly increased AMF colonization of typha roots up to 16.6% and 21.8% respectively. The length, number of leaves and biomass of typha also increased up to 90%, 50% and 97% respectively compared to those without AMF inoculation. G. etunicatum contributed the best growth of typha grown in mixed compost-soil-tailings, resulting in double increase of its length and biomass. The application of G. manihotis did not significantly increase the growth of typha in mixed media; however, under pure tailing, this mycorrhiza had the best induction for typha biomass and leaf number. In general, AMF application increased growth of typha grass in tailings media.

2020 ◽  
Vol 21 (2) ◽  
Author(s):  
Luluk Setyaningsih ◽  
FIRLI AZHAR DIKDAYATAMA ◽  
ARUM SEKAR WULANDARI

Abstract. Setyaningsih L, Dikdayatama FA, Wulandari AS. 2020. Arbuscular mycorrhizal fungi and Rhizobium enhance the growth of Samanea saman (trembesi) planted on gold-mine tailings in Pongkor, West Java, Indonesia. Biodiversitas 21: 611-616. Revegetation of severely degraded lands, such as gold-mine tailings, requires comprehensive approach including the selection of appropriate tree species and the improvement of soil fertility with the application of microorganisms. This study aimed to analyze the growth of trembesi (Samanea saman (Jacq.) Merr) seedlings inoculated with Arbuscular Mycorrhizal Fungi (AMF) and rhizobial bacteria (RB) on gold-mine tailings in Pongkor, Bogor, West Java, Indonesia. The AMF, RB, and mixture of AMF and RB were inoculated into S. saman seedlings and then the seedlings were grown on gold mine tailings land. Seedling growth, AMF infection and RB nodulation were observed after 12 weeks after planting. The results showed that the treatments facilitated AMF colonization by up to 20.7% and RB nodulation up to 22 nodules per plant. The inoculation of S. saman seedlings with AMF and RB significantly increased the growth on diameter, biomass, and root length, with values of root-to-shoot ratio of 3.1-4.3. The roots of S. saman seedlings were also able to penetrate the depth of the tailings solum. These results showed that AMF and RB application effectively increased the growth of S. saman seedlings in the gold tailings field.


2018 ◽  
Vol 1 (1) ◽  
Author(s):  
John Paul J. Aseniero ◽  
Einstine M. Opiso ◽  
Marybeth Hope T. Banda ◽  
Carlito B. Tabelin

2018 ◽  
Vol 19 (4) ◽  
pp. 1294-1302 ◽  
Author(s):  
MUHAMMAD HILMI ◽  
HAMIM HAMIM ◽  
YOHANA C. SULISTYANINGSIH ◽  
TAUFIKURAHMAN TAUFIKURAHMAN

Hilmi M, Hamim H, Sulistyaningsih YC, Taufikurahman. 2018. Growth, histochemical and physiological responses of nonedibleoil producing plant (Reutealis trisperma) to gold mine tailings. Biodiversitas 19: 1294-1302. Reutealis trisperma (Blanco) AiryShaw is a non-edible biodiesel producing plant that is able to grow well in various unfavorable environmental conditions. The studyaimed to analyze the growth, physiological, and anatomical responses of R. trisperma to gold mine tailings. Three-month-old of R.trisperma were grown in 8 kg of polybags contained with mixed soil-compost medium treated with 0, 25, 50 and 100% of gold minetailings for 3 months. Root and shoot growth, physiological and anatomical characters, and histochemical analysis of Pb inside the rootsand leaves were examined. The root and shoot growth as well as chlorophyll a and b contents of R. trisperma grown in sole gold minetailing at 100% significantly decreased, while at the lower concentration of gold mine tailings, the decrease of the growth performanceswas not significant, or even increased shown in that of 25% of tailing treatment. The treatment of gold mine tailing at 100% alsoinduced lipid peroxidation, indicated by the significant increase in malondialdehyde (MDA) contents in the root as well as the leaves.Histochemical analysis showed that accumulation of Pb occurred both in roots as well as in leaves of R. trisperma treated with 100% oftailings. High-level tailing treatment also induced anatomical alteration in roots as well as leaves of the species. These results indicatedthat gold mine tailings induced oxidative stress in roots and leaves of R. trisperma resulted in growth inhibition.


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