Coat protein gene of a PStV-Bm isolate from West Nusa Tenggara, Indonesia

Abstract. Julisaniah NI, Suharjono, Mastuti R, Arumingtyas EL. 2020. Coat protein gene of a PStV-Bm isolate from West Nusa Tenggara, Indonesia. Biodiversitas 21: 903-909. Peanut stripe virus (PStV) is a single-stranded positive-sense RNA virus capable of infecting peanut plants. An isolate of PStV (PStV-Bm) was collected from a peanut field in the Bima District, West Nusa Tenggara Province, Indonesia and the coat protein (CP) gene of this virus (CP-PStV) was extracted from the viral RNA and analyzed using reverse transcription-polymerase chain reaction methods. The CP-PStV gene of PStV-Bm was aligned with several PStV genes deposited in the Genbank (http://www.ncbi.nml.nih.gov). Based on the nucleotide sequence of the CP gene, PStV-Bm was grouped into a similar cluster with other PStVs that originated from Indonesia with a similar index, ranging from 96.8% to 98.9%. Genetic similarity (about 96.1%) was also observed between PStV-Bm and PStV from the USA. This genetic similarity indicated that viruses from adjacent regions have high genetic relationships. Some amino acid differences were observed in PStV-Bm that may be typical of this strain.

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Bean common mosaic virus and Bean common mosaic necrosis virus in Mexico

Plant Disease ◽

10.1094/pdis.2003.87.1.21 ◽

2003 ◽

Vol 87 (1) ◽

pp. 21-25 ◽

Cited By ~ 28

Author(s):

N. Flores-Estévez ◽

J. A. Acosta-Gallegos ◽

L. Silva-Rosales

Keyword(s):

Coat Protein ◽

Mosaic Virus ◽

Coat Protein Gene ◽

Protein Gene ◽

Bean Common Mosaic Virus ◽

Nucleotide Sequences ◽

Necrosis Virus ◽

Cp Gene

A survey was performed in Mexico to study the distribution of Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) using a set of primers directed to the coat protein gene (CP) that were designed to detect and characterize the two viral species. Both viral species were present in different locations in the country. BCMV was predominant in the central states of the country, whereas BCMNV proliferated toward the eastern tropical states. The alignment of nine nucleotide sequences for each viral species at the amino region of the CP gene confirmed the identities of the viruses and set the basis to assign them tentatively to pathogroups I, II, and VI.

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PENGKLONAN DAN PERUNUTAN NUKLEOTIDA GEN SELUBUNG PROTEIN DAN 3’UTR (untranslated region) PEANUT STRIPE VIRUS

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.111-6 ◽

2001 ◽

Vol 1 (1) ◽

pp. 1-6

Author(s):

Hasriadi Mat Akin

Keyword(s):

Amino Acid ◽

Coat Protein ◽

Coat Protein Gene ◽

Cleavage Site ◽

Untranslated Region ◽

Protein Gene ◽

Start Codon ◽

Genomic Rna ◽

Cp Gene ◽

Peanut Stripe Virus

Cloning and sequencing of coat protein gene and 3’UTR (untranslated region) of peanut stripe virus. The cDNA of 3' terminal of peanut stripe virus genomic RNA was cloned and sequenced. The cDNA was ligated with plasmid vector pGEM-T Easy and transformed to competent cells of Escherichia coli. The 3' terminal of PstV genomic RNA contained 1195 nucleotides (nts). The region included the nucleotide sequences of NIb (nuclear inclusion body) (129 nts), CP gene (coat protein gene) (861 nts), and 3'UTR (untranslated region) (205 nts). The nucleotide sequence of a CP gene contained one long uninterrupted open reading frame (ORF) without a start codon, which ended a UAG stop codon. The 287 amino acid residues of PStV coat protein were predicted from the CP gene. The amino acid was analyzed for the presence of consensus polyprotein cleavage site for maturation of potyvirus polyprotein. A putative cleavage site was found at position 43 (Q/S) following the Valine (V) residue at -4 position. This isolate of PstV can be expected to be aphid transmissible because the coat protein contained a DAG triplet at position 53-55.

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Coat protein gene (Cp gene)

The Dictionary of Genomics, Transcriptomics and Proteomics ◽

10.1002/9783527678679.dg02030 ◽

2015 ◽

pp. 1-1

Keyword(s):

Coat Protein ◽

Coat Protein Gene ◽

Protein Gene ◽

Cp Gene

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065 Genetic Stability of Potato Leafroll Virus Coat Protein Gene in Transgenic Potatoes

HortScience ◽

10.21273/hortsci.35.3.399e ◽

2000 ◽

Vol 35 (3) ◽

pp. 399E-399

Author(s):

Hyo-Won Seo ◽

Jung-Yoon Yi ◽

Young-Il Hahm ◽

Hyun-Mook Cho ◽

Kuen Woo Park

Keyword(s):

Coat Protein ◽

Irish Cobbler ◽

Coat Protein Gene ◽

Genetic Stability ◽

Protein Gene ◽

Transgenic Potato ◽

Putative Transformants ◽

Dna And Rna ◽

Cp Gene ◽

Transgenic Lines

Three potato (Solanum tuberosum L.) cultivars `Superior', `Irish Cobbler', and `Jopung' were transformed by co-cultivation with tuber discs and disarmed Agrobacterium tumefaciens LBA4404 carrying modified vector pBI121, that contained PLRV coat protein (CP) gene and controlled by CaMV35S promoter. Putative transformants were selected and their genomic DNA and RNA transcripts were analyzed for the confirmation of genetic stability by RT-PCR, PCR, southern, and northern blot. The growth characteristics and viral resistance of progenies of transgenic potato plants were investigated. Twelve lines among the different seven-times manipulated transgenic lines were grown in greenhouse and isolates trial field. PLRV coat protein gene was stably inherited in `Superior', but not in `Jopung'. `Jopung' was less stable than `Irish Cobbler' and `Superior' at genetic stability of PLRV CP gene. And some of these transgenic lines were highly resisted in PLRV multiplication. The yield of transformants was reduced in `Irish Cobbler' but not in `Superior'. Possible explanations for these types of resistance are gene silencing and positional effects of transformed PLRV CP genes and that had cultivar specificity. We consider the appearance of escaped transformants in `Jopung' for emergence of chimeric explants from early selection stage.

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TRANSGENE IDENTITY AND NUMBER OF INTEGRATION SITES AND THEIR CORRELATION WITH RESISTANCE TO PStV IN TRANSGENIC PEANUTS CARRYING PEANUT STRIPE VIRUS (PStV) COAT PROTEIN GENE

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.1739-47 ◽

2007 ◽

Vol 7 (1) ◽

pp. 39-47

Author(s):

Dwi Hapsoro ◽

Hajrial Aswidinnoor ◽

Rusmilah Suseno ◽

Jumanto Jumanto ◽

Sudarsono Sudarsono

Keyword(s):

Coat Protein ◽

Coat Protein Gene ◽

Copy Number ◽

Protein Gene ◽

Insertion Site ◽

Southern Analysis ◽

Cp Gene ◽

Peanut Stripe Virus ◽

Integration Sites ◽

Transgenic Peanut

Transgene Identity and Number of Integration Sites and Their Correlation with Resistance To PStV in Transgenic Peanuts Carrying Peanut Stripe Virus (PStV) Coat Protein Gene. This research aimed to determine (1) the identity and copy number of PStV cp gene in transgenic peanut plants carrying PStV cp gene and (2) correlation between the identity and the number of integration sites and resistance to PStV infection. One T0 transgenic peanut was selfed up to five generations. T2, T3, and T5 plants were mechanically inoculated with PStV. Samples of T5 plants derived from several different T4 plants were subjected to Southern analysis to confirm the integration of PStV cp gene and to determine its identity and copy number. The Southern analysis showed three bands of different size, i.e. 1.1 kb, 1.3 kb, and 5.8 kb. Most of the lines of T5 generation have one insertion site, suggesting that the three insertion sites were located in different loci. Based on the phenotypic data, the transgenes of 1.1 kb and 1.3 kb were functional, resulting in resistant or recovery phenotype, while that of 5.8 kb was not functional. Copy number apparently had no effects on the phenotypes.

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Coat protein gene duplication in a filamentous RNA virus of plants.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.89.19.9156 ◽

1992 ◽

Vol 89 (19) ◽

pp. 9156-9160 ◽

Cited By ~ 43

Author(s):

V. P. Boyko ◽

A. V. Karasev ◽

A. A. Agranovsky ◽

E. V. Koonin ◽

V. V. Dolja

Keyword(s):

Coat Protein ◽

Gene Duplication ◽

Coat Protein Gene ◽

Protein Gene ◽

Rna Virus

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EKSPRESI GEN PROTEIN SELUBUNG TOMATO INFECTIOUS CHLOROSIS VIRUS PADA ESCHERICHIA COLI

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.215114-121 ◽

2016 ◽

Vol 15 (2) ◽

pp. 114

Author(s):

Fitrianingrum Kurniawati ◽

Gede Suastika ◽

Giyanto .

Keyword(s):

Escherichia Coli ◽

Coat Protein ◽

Coat Protein Gene ◽

Protein Gene ◽

Serological Test ◽

Coli Strain ◽

Rt Pcr ◽

Cp Gene ◽

Sds Page ◽

Tomato Infectious Chlorosis Virus

Expression of tomato infectious chlorosis virus coat protein gene on Escherichia coli. Tomato infectious chlorosis virus (TICV) is the causal agent of chlorotic disease of tomato. Detection of TICV can be carried out by RT-PCR and serological test. Titer of TICV in plant tissue is very low because TICV is limited to phloem. Serological detection of TICV requires antiserum which is not available in Indonesia. Producing antibody through cloning and coat protein gene (TICV CP gene) expression is a promising approach in producing antiserum. The objective of this study was to express TICV CP gene as antigen for antiserum production. TICV CP gene was amplified using RT-PCR from total RNA extracted from TICV infected leaves collected from Cipanas, Cianjur, West Java. The amplified CP gene was then sequenced and sub-cloned into pET 21b expression vector, transformed into Escherichia coli strain BL21 DE3(pLysS) and induced expression using IPTG 1 mM overnight at 37 °C. CP that contains 6xhistag was purified using NiNTAspin column and then confirmed by SDS-PAGE. The size of TICV CP gene was 750 bp and the gene was expressed on pET 21 b vector and SDS-PAGE showed a 29 kDa band.

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