065 Genetic Stability of Potato Leafroll Virus Coat Protein Gene in Transgenic Potatoes

Three potato (Solanum tuberosum L.) cultivars `Superior', `Irish Cobbler', and `Jopung' were transformed by co-cultivation with tuber discs and disarmed Agrobacterium tumefaciens LBA4404 carrying modified vector pBI121, that contained PLRV coat protein (CP) gene and controlled by CaMV35S promoter. Putative transformants were selected and their genomic DNA and RNA transcripts were analyzed for the confirmation of genetic stability by RT-PCR, PCR, southern, and northern blot. The growth characteristics and viral resistance of progenies of transgenic potato plants were investigated. Twelve lines among the different seven-times manipulated transgenic lines were grown in greenhouse and isolates trial field. PLRV coat protein gene was stably inherited in `Superior', but not in `Jopung'. `Jopung' was less stable than `Irish Cobbler' and `Superior' at genetic stability of PLRV CP gene. And some of these transgenic lines were highly resisted in PLRV multiplication. The yield of transformants was reduced in `Irish Cobbler' but not in `Superior'. Possible explanations for these types of resistance are gene silencing and positional effects of transformed PLRV CP genes and that had cultivar specificity. We consider the appearance of escaped transformants in `Jopung' for emergence of chimeric explants from early selection stage.

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Restoration of Wild-Type Virus by Double Recombination of Tombusvirus Mutants with a Host Transgene

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.1999.12.2.153 ◽

1999 ◽

Vol 12 (2) ◽

pp. 153-162 ◽

Cited By ~ 50

Author(s):

Marise Borja ◽

Teresa Rubio ◽

Herman B. Scholthof ◽

Andrew O. Jackson

Keyword(s):

Coat Protein ◽

Transgenic Plants ◽

Coat Protein Gene ◽

Protein Gene ◽

Wild Type Virus ◽

Type Virus ◽

Virus Infections ◽

Wild Type ◽

Transgenic Lines ◽

Double Recombination

Nicotiana benthamiana plants transformed with the coat protein gene of tomato bushy stunt virus (TBSV) failed to elicit effective virus resistance when inoculated with wild-type virus. Subsequently, R1 and R2 progeny from 13 transgenic lines were inoculated with a TBSV mutant containing a defective coat protein gene. Mild symptoms typical of those elicited in nontransformed plants infected with the TBSV mutant initially appeared. However, within 2 to 4 weeks, up to 20% of the transgenic plants sporadically began to develop the lethal syndrome characteristic of wild-type virus infections. RNA hybridization and immunoblot analyses of these plants and nontransformed N. benthamiana inoculated with virus from the transgenic lines indicated that wild-type virus had been regenerated by a double recombination event between the defective virus and the coat protein transgene. Similar results were obtained with a TBSV deletion mutant containing a nucleotide sequence marker, and with a chimeric cucumber necrosis virus (CNV) containing the defective TBSV coat protein gene. In both cases, purified virions contained wild-type TBSV RNA or CNV chimeric RNA derived by recombination with the transgenic coat protein mRNA. These results thus demonstrate that recombinant tombusviruses can arise frequently from viral genes expressed in transgenic plants.

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PVYNTN-CP coat protein gene mediated virus resistance of transgenic potato plants

Ecological genetics ◽

10.17816/ecogen7441-50 ◽

2009 ◽

Vol 7 (4) ◽

pp. 41-50 ◽

Cited By ~ 1

Author(s):

Zenon Stasevski ◽

Olga N Ilinskaya

Keyword(s):

Coat Protein ◽

Coat Protein Gene ◽

Virus Resistance ◽

Protein Gene ◽

Solanum Tuberosum L ◽

Transgenic Potato ◽

Potato Plants ◽

Transgenic Potato Plants ◽

Transgenic Potatoes ◽

Virus Strains

PVYNTN-CP coat protein gene from a necrotic strain of potato virus Y (pvyntn) has been transferred into two potato Solanum tuberosum L. cultivars Mindenes and Somogyi kifli via Agrobacterium tumefaciens transformation. Expression of integrated PVYNTN-CP gene were confirmed for 33 (89 %) of 37 and 3 (75 %) of 4 kanamycin-resistant regenerants of potato cultivars Mindenes and Somogyi kifli respectively. The level of virus resistance against two virus strains (PVY°, PVYNTN) of independent lines of transgenic potatoes varied between extreme resistance to susceptibility. The three independent lines of transgenic potatoes proved to be extreme resistant against both PVY strains.

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Coat protein gene of a PStV-Bm isolate from West Nusa Tenggara, Indonesia

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210308 ◽

2020 ◽

Vol 21 (3) ◽

Author(s):

Nur Indah Julisaniah ◽

SUHARJONO ◽

RETNO MASTUTI ◽

ESTRI LARAS ARUMINGTYAS

Keyword(s):

Coat Protein ◽

Coat Protein Gene ◽

Genetic Similarity ◽

Protein Gene ◽

Rna Virus ◽

Genetic Relationships ◽

Cp Gene ◽

Similar Cluster ◽

Similar Index ◽

The Usa

Abstract. Julisaniah NI, Suharjono, Mastuti R, Arumingtyas EL. 2020. Coat protein gene of a PStV-Bm isolate from West Nusa Tenggara, Indonesia. Biodiversitas 21: 903-909. Peanut stripe virus (PStV) is a single-stranded positive-sense RNA virus capable of infecting peanut plants. An isolate of PStV (PStV-Bm) was collected from a peanut field in the Bima District, West Nusa Tenggara Province, Indonesia and the coat protein (CP) gene of this virus (CP-PStV) was extracted from the viral RNA and analyzed using reverse transcription-polymerase chain reaction methods. The CP-PStV gene of PStV-Bm was aligned with several PStV genes deposited in the Genbank (http://www.ncbi.nml.nih.gov). Based on the nucleotide sequence of the CP gene, PStV-Bm was grouped into a similar cluster with other PStVs that originated from Indonesia with a similar index, ranging from 96.8% to 98.9%. Genetic similarity (about 96.1%) was also observed between PStV-Bm and PStV from the USA. This genetic similarity indicated that viruses from adjacent regions have high genetic relationships. Some amino acid differences were observed in PStV-Bm that may be typical of this strain.

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Resistance in Transgenic Potato Expressing the Potato Leafroll Virus Coat Protein Gene

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-3-301 ◽

1990 ◽

Vol 3 (5) ◽

pp. 301 ◽

Cited By ~ 78

Author(s):

L. M. Kawchuk

Keyword(s):

Coat Protein ◽

Coat Protein Gene ◽

Protein Gene ◽

Transgenic Potato ◽

Potato Leafroll Virus ◽

Virus Coat Protein ◽

Virus Coat ◽

Leafroll Virus

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Bean common mosaic virus and Bean common mosaic necrosis virus in Mexico

Plant Disease ◽

10.1094/pdis.2003.87.1.21 ◽

2003 ◽

Vol 87 (1) ◽

pp. 21-25 ◽

Cited By ~ 28

Author(s):

N. Flores-Estévez ◽

J. A. Acosta-Gallegos ◽

L. Silva-Rosales

Keyword(s):

Coat Protein ◽

Mosaic Virus ◽

Coat Protein Gene ◽

Protein Gene ◽

Bean Common Mosaic Virus ◽

Nucleotide Sequences ◽

Necrosis Virus ◽

Cp Gene

A survey was performed in Mexico to study the distribution of Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) using a set of primers directed to the coat protein gene (CP) that were designed to detect and characterize the two viral species. Both viral species were present in different locations in the country. BCMV was predominant in the central states of the country, whereas BCMNV proliferated toward the eastern tropical states. The alignment of nine nucleotide sequences for each viral species at the amino region of the CP gene confirmed the identities of the viruses and set the basis to assign them tentatively to pathogroups I, II, and VI.

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Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection

Plant Molecular Biology ◽

10.1007/bf00040637 ◽

1991 ◽

Vol 17 (3) ◽

pp. 431-439 ◽

Cited By ~ 54

Author(s):

Frank van der Wilk ◽

Dinie Posthumus-Lutke Willink ◽

Marianne J. Huisman ◽

Harm Huttinga ◽

Rob Goldbach

Keyword(s):

Coat Protein ◽

Viral Infection ◽

Coat Protein Gene ◽

Protein Gene ◽

Transgenic Potato ◽

Potato Plants ◽

Transgenic Potato Plants ◽

Potato Leafroll Luteovirus

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PENGKLONAN DAN PERUNUTAN NUKLEOTIDA GEN SELUBUNG PROTEIN DAN 3’UTR (untranslated region) PEANUT STRIPE VIRUS

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.111-6 ◽

2001 ◽

Vol 1 (1) ◽

pp. 1-6

Author(s):

Hasriadi Mat Akin

Keyword(s):

Amino Acid ◽

Coat Protein ◽

Coat Protein Gene ◽

Cleavage Site ◽

Untranslated Region ◽

Protein Gene ◽

Start Codon ◽

Genomic Rna ◽

Cp Gene ◽

Peanut Stripe Virus

Cloning and sequencing of coat protein gene and 3’UTR (untranslated region) of peanut stripe virus. The cDNA of 3' terminal of peanut stripe virus genomic RNA was cloned and sequenced. The cDNA was ligated with plasmid vector pGEM-T Easy and transformed to competent cells of Escherichia coli. The 3' terminal of PstV genomic RNA contained 1195 nucleotides (nts). The region included the nucleotide sequences of NIb (nuclear inclusion body) (129 nts), CP gene (coat protein gene) (861 nts), and 3'UTR (untranslated region) (205 nts). The nucleotide sequence of a CP gene contained one long uninterrupted open reading frame (ORF) without a start codon, which ended a UAG stop codon. The 287 amino acid residues of PStV coat protein were predicted from the CP gene. The amino acid was analyzed for the presence of consensus polyprotein cleavage site for maturation of potyvirus polyprotein. A putative cleavage site was found at position 43 (Q/S) following the Valine (V) residue at -4 position. This isolate of PstV can be expected to be aphid transmissible because the coat protein contained a DAG triplet at position 53-55.

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