scholarly journals Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum

2021 ◽  
Vol 22 (6) ◽  
Author(s):  
WIKO ARIF WIBOWO ◽  
SIGIT DWI MARYANTO ◽  
BUDI SETIADI DARYONO
Keyword(s):  
Molecular Characterization ◽  
Cucumis Melo ◽  
Plant Genetic Resources ◽  
Chromosome 1 ◽  
Sequencing Analysis ◽  
Ppiase Activity ◽  
Phenotypic Characters ◽  
Quantitative Characters ◽  
Qualitative Characters ◽  
Cucumis Melo L

Abstract. Maryanto SD, Wibowo WA, Daryono BS. 2021. Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum. Biodiversitas 22: 3007-3014. Cucumis melo L. cv. Gama Melon Parfum is a new cultivar with a very strong fragrance as its main character. As a new cultivar with unique characters, it is necessary to characterize phenotype and molecular related to the fragrant aroma. The research aimed to study the phenotypic of the fruit of C. melo cv. Gama Melon Parfum (F3) and to identify the CYPs gene as one of the genes that act in encoding volatile compounds. Analysis of qualitative characters was based on International Plant Genetic Resources Institute (IPGRI) and Plant Variety Protection (PVP), phenotypic characters in melons observed by abiding the Rules for Registration of Varieties from the Indonesian Minister of Agriculture Decree No. 700/Kpts/OT.320/D/12/2011, while analysis of quantitative characters was using the ANOVA methods and software PKBT-STAT-2. The methods of molecular characterization included RNA isolation, cDNA synthesis used Reverse Transcriptase-PCR, amplification of DNA target used PCR, visualization of DNA target used electrophoresis, and DNA sequencing. Analysis of the in silico approach was carried out on the CuGenDB Melon database. Analysis of protein sequences and classification was obtained from InterPro. Phylogeny analysis using MEGA-X Software. The results were 18 qualitative characters and 11 quantitative characters were stable and uniform, whereas the molecular characterization of the genes was predicted Cyclophilin with peptidylprolyl isomerase (PPIase) activity and located in chromosome 1 (17059021-17058899).

scholarly journals The effect of Begomovirus infection on phenotypic characters of Cucumis melo L. ‘Melona’

2020 ◽  
Author(s):  
Rizal Hermawan Setiyobudi ◽  
Aprilia Sufi Subiastuti ◽  
Budi Setiadi Daryono
Keyword(s):  
Cucumis Melo ◽  
Phenotypic Characters ◽  
Begomovirus Infection ◽  
Cucumis Melo L

Molecular characterization of South and East Asian melon, Cucumis melo L., and the origin of Group Conomon var. makuwa and var. conomon revealed by RAPD analysis

Euphytica ◽  
2006 ◽  
Vol 153 (1-2) ◽  
pp. 233-247 ◽  
Author(s):  
Katsunori Tanaka ◽  
Atsushi Nishitani ◽  
Yukari Akashi ◽  
Yoshiteru Sakata ◽  
Hidetaka Nishida ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Cucumis Melo ◽  
Rapd Analysis ◽  
East Asian ◽  
Cucumis Melo L

scholarly journals Primeiro ciclo de seleção massal na população PM3 de melão (Cucumis melo L)

2015 ◽  
Vol 10 (4) ◽  
pp. 21 ◽  
Author(s):  
L. S. Medeiros ◽  
P. V. Ferreira ◽  
I. D. E. de Carvalho ◽  
F. S. Oliveira ◽  
J. Silva
Keyword(s):  
Cucumis Melo ◽  
Fruit Shape ◽  
Mass Selection ◽  
Transverse Diameter ◽  
Selection Cycle ◽  
Phenotypic Characters ◽  
Class Weight ◽  
Cucumis Melo L ◽  
Longitudinal Diameter ◽  
Selection Of

<p>Objetivou-se com este trabalho realizar o primeiro ciclo de seleção massal na população PM3 de melão. Foi usada a população PM3 de melão, obtida pelo Setor de Melhoramento Genético de Plantas do Centro de Ciências Agrárias da Universidade Federal de Alagoas (SMGP/CECA/UFAL). A colheita foi realizada aos 75 dias após a semeadura. Os frutos foram colhidos totalizaram 1188 frutos, os quais foram analisados a morfologia externa, objetivando a formação de classes fenotípicas em função dos caracteres qualitativos, culminando na obtenção de 40 classes fenotípicas. A classe fenotípica CF 17 foi selecionada por apresentar cor do fruto amarelo, ausência de costelas, ausência de rendilhamento, cor da polpa salmão e formato do fruto arredondado a ovalado. Em seguida foi feita uma seleção dos frutos de melão para os seguintes caracteres quantitativos dentro da classe fenotípica selecionada: peso do fruto acima de 740,0 gramas; diâmetro longitudinal acima de 11,0 cm; diâmetro transversal acima de 10,0 cm, espessura da polpa acima de 1,9 cm; espessura da casca acima de 0,2 cm; diâmetro da cavidade menor que 8,0 cm. A classe fenotípica CF 17 foi à única selecionada.</p><p align="center"><strong><em>First mass selection cycle in melon PM3 population (Cucumis melo L) </em></strong></p><p align="center"><strong><em> </em></strong></p><p class="Estiloestilo1esquerda">Abstract: The objective of this work make the first mass selection cycle in melon PM3 population. It was used to melon PM3 population, obtained by the sector of improvement genetic of plants of center science land the Federal University of Alagoas (SMGP/CECA/UFAL). Plants were harvested at 75 days after sowing. The fruits were harvested totaled 1188 fruits, which were analyzed the external morphology, aiming at the formation of phenotypic classes on the basis of qualitative character, culminating in getting 40 phenotypic classes. Phenotypic class CF 17 has been selected for display color of yellow fruit, no ribs, no rendilhamento, color of salmon flesh and rounded to oval fruit shape. Then a selection of melon fruit was made to the following quantitative phenotypic characters within the selected class weight of the fruit above 740.0 grams; longitudinal diameter greater than 11.0 cm; transverse diameter greater than 10.0 cm, flesh thickness above 1.9 cm; shell thickness above 0.2 cm; cavity diameter smaller than 8.0 cm. Phenotypic class CF 17 was the selected one.      </p>

scholarly journals Molecular Characterization and Phylogenetic Relationships of Melon (Cucumis melo L. Meloni Cultivar) Based on Expression of Andromonoecious Gene

2020 ◽  
Vol 27 (2) ◽  
pp. 174
Author(s):  
Eka Wasi’ Al-Mughni ◽  
Budi Setiadi Daryono
Keyword(s):  
Gene Expression ◽  
Phylogenetic Analysis ◽  
Phylogenetic Tree ◽  
Molecular Characterization ◽  
Phylogenetic Relationships ◽  
Cucumis Melo ◽  
Specific Primer ◽  
Economic Prospect ◽  
Cucumis Melo L

Melon (Cucumis melo L.) is one of horticultural commodities with good economic prospect for melon farmers because of several advantages. 'Meloni' is result of crossing between ‘Sun Lady-3’ and ‘PI 371795’ by Genetics and Breeding Laboratory, Faculty of Biology, UGM. The 'Meloni' plant produces females flowers that expressed by CmACS-7 gene and it can be expected that melon plants 'Meloni' included in the primitive cultivars, while 'Meloni' is a cultivation cultivar. The aim of this research is to study the relation of 'Meloni' kinship to primitive and modern cultivars. Characterization of the CmACS-7 gene is begun by a total isolation process of RNA from a sample of melon plant flowers and detection using a specific primer. The cDNA bands obtained are sequenced and then phylogenetic analysis is performed based on CmACS-7 gene expression using MEGA 7.0. Result of this study show that the presence of the CmACS-7 gene in female and hermaphrodite flowers of ‘Meloni’, ‘PI 371795’, ‘Sun Lady-3’, and ‘Hikapel’. Phylogenetic tree analyze show that ‘Meloni’ from female plant, ‘PI 371795’ and ‘Sun Lady-3’ have closely relation, while 'Hikapel' separated from other cultivars.

Modelo productivo del cultivo de melón (Cucumis melo L.) para la región Caribe

2017 ◽  
Author(s):  
César Elías Baquero Maestre ◽  
Ángela Arcila Cardona ◽  
Heriberto Arias Bonilla ◽  
Marlon Yacomelo Hernández
Keyword(s):  
Cucumis Melo ◽  
Cucumis Melo L
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