A review chromosome analysis comparison of swamp buffalo (Bubalus bubalis) with different phenotypic characters in East Java, Indonesia

Chromosomal data on swamp buffalo especially in East Java province, is still lacking. For breeders, chromosomal analysis needs to be done to detect the possibility of hereditary genetic defects. In Malang Area, farmers lack of care and breeding programs for swamp buffalo also caused a decline in the genetic quality and performance. The purpose of this study was to compare the results of the chromosome analysis of swamp buffalo in Malang and another area in East Java province, which were selected based on differences in phenotypic characters. Chromosome analysis is very important for breeders because the results of this study can be used as a consideration for strategies to improve the genetic quality of swamp buffalo. The method used is standard karyotyping using whole blood with G banding staining. Chromosomal preparation using Karyo MAX medium, Colcemic Solution, Giemsa Stain, and KCl solution. Cell culture was performed according to the standard karyotyping method in mammals. The minimum number of 5 spreading chromosomes is the best was chosen, microphotographed, and then chromosome analysis is performed using Cytovision Image Analysis software. The results of this study did not find swamp buffalo with an abnormal number of chromosomes in East Java Province, Indonesia.

Systematic Revision of a New Butterfly Genus, Cisandina Nakahara & Espeland, n. gen., with Descriptions of Three New Taxa (Lepidoptera: Nymphalidae: Satyrinae)

We here establish a new genus in the nymphalid butterfly subtribe Euptychiina, Cisandina Nakahara & Espeland, n. gen. to harbor five species hitherto placed within two polyphyletic genera, namely Magneuptychia Forster, 1964 and Euptychoides Forster, 1964. We compiled data from over 350 specimens in 17 public and private collections, as well as DNA sequence data for all relevant species, to revise the species-level classification of this new genus. According to our multi-locus molecular phylogeny estimated with the maximum likelihood approach, Cisandina lean. comb., Cisandina philippan. comb. & reinst. stat., Cisandina fidan. comb., Cisandina sanmarcosn. comb., and Cisandina trinitensisn. comb. are proposed as new taxonomic combinations, since these species are distantly related to the type species of Magneuptychia and Euptychoides and cannot reasonably be accommodated in any other genus. Lectotypes are designated for Papilio lea Cramer, 1777, Papilio junia Cramer, 1780, Euptychia philippa Butler, 1867, and Eupytchia fida Weymer, 1911. Two new species of Cisandinan. gen. are named and described herein, C. esmeralda Nakahara & Barbosa, n. sp. and C. castanya Lamas & Nakahara, n. sp., increasing the described species diversity of the genus to seven. The immature stages of C. castanyan. sp. and C. philippan. comb. & reinst. stat. are documented along with their natural hostplants, representing the first two species of the genus with known life history information. We describe a new subspecies, Cisandina fida directa Nakahara & Willmott, n. ssp., based on a limited number of specimens from southern Ecuador and central Peru. We were unable to obtain genetic data for the nominate race of C. fidan. comb., and thus, this taxonomic hypothesis is currently based solely on phenotypic characters.

Morphological Variability of Mangosteen in Bengkulu Province (Variabilitas Morfologi Manggis di Provinsi Bengkulu)

<p>Mangosteen is a genetic resource scattered and cultivated by farmers in Bengkulu Province. The study aimed at determining the morphological variability of mangosteen. The study was conducted from March to October 2015 in Bengkulu province using on-site supervision and observation methods. The number of plants observed was 34 genotypes and qualitatively and quantitatively characterized based on the Center for Plant Variety Protection and Agriculture Licensing (CPVPAL) guide. The data of morphology were analyzed by comparing the average value, variance, and standard deviation. Relationship or data grouping phenotypic characters appearance were identified using NTSYS software version 2.1. The results show wide variability in the density of leaf, flower size, and fruit weight, while narrow variability is occurred in plant height and the thickness of the rind. Data analysis of mangosteen genotypes generated similarity coefficients range from 0.71 to 0.91 (71-91%). Genotype 24 and 27 have the closest relationship, with the similarity coefficient of 91%.</p><p><strong>Keywords</strong></p><p>Mangosteen; Morphology; Variability; Character; Phenotypic</p><p><strong>Abstract</strong></p><p>Manggis merupakan sumber daya genetik yang tersebar dan dibudidayakan oleh petani di Provinsi Bengkulu. Penelitian bertujuan untuk mengetahui variabilitas fenotipik buah manggis. Penelitian dilaksanakan pada bulan Maret sampai Oktober 2015 di Provinsi Bengkulu dengan menggunakan metode observasi langsung morfologi tanaman manggis yang telah berbuah. Jumlah tanaman yang diamati sebanyak 34 genotipe dan masing-masing genotipe diamati karakterisasi kualitatif dan kuantitatif berdasarkan panduan Pusat Perlindungan Varietas Tanaman dan Perizinan Pertanian (PPVTPP). Analisis data melalui perbandingan varian fenotipik dengan standar deviasi varian fenotipe diukur dengan nilai rata-rata, varians, dan standar deviasi. Kekerabatan atau pengelompokan data penampilan karakter fenotipik dilakukan dengan menggunakan software NTSys versi 2.1. Hasil penelitian menunjukkan bahwa variabilitas luas terdapat pada kerapatan daun, ukuran bunga, dan bobot buah, sedangkan variabilitas sempit pada karakter tinggi tanaman dan ketebalan kulit buah. Analisis genotipe manggis menghasilkan koefisien kemiripan sebesar 0,71-0,91 (71-91%). Dari dendogram diperoleh genotipe yang memiliki tingkat kekerabatan yang tinggi terdapat pada genotipe 24 dan 27 dengan nilai kemiripan sebesar 91%.<strong></strong></p>

General patterns of sexual dimorphism in graylings (Thymallus), with a comparison to other salmonid species

Among fishes, salmonids (family Salmonidae) have attracted a great deal of research attention focused on sexual dimorphism and associated selective forces. Most of this research has been directed toward anadromous and mostly semelparous salmon and trout (Oncorhynchus, Salmo), and comparatively little is known about intersexual variability in strictly iteroparous freshwater salmonids. We examined a comprehensive data set of 28 linear morphometric characters in 11 of 15 currently recognised species of grayling (Thymallinae, Thymallus), a genus consisting of iteroparous species only, to identify general patterns of intersexual morphological variability. Overall, we found that all grayling species show common sex-specific traits particularly relating to size dimensions of the dorsal, anal, pelvic and pectoral fins. Although the magnitude of sexual dimorphism differed among species, there was no significant phylogenetic signal associated with these differences across the genus. These results are discussed in terms of the assumed selection pressures driving sexual dimorphism in graylings and are compared to existing knowledge in Salmonidae as a whole where similarities and differences with both Salmoninae and Coregoninae exist. The present study provides the first detailed genus-wide comparison of sexually dimorphic phenotypic characters in graylings, and highlights the need for more large-scale comparative studies in multiple salmonid species to better understand general macroevolutionary trends among this important group of freshwater fishes.

A mikroorganizmusok patogenitása és virulenciája: definíciók, példázatok és molekuláris háttérismeretek

Összefoglaló. A fertőző betegségek kóroki hátterének felderítésére irányuló törekvések hosszú időre tekintenek vissza. Fogalmakkal és követelményekkel (posztulátumokkal) igyekeztek körülírni, hogy egy mikroorganizmus mikor tekinthető egy adott fertőző betegség okozójának. Egy patogén rendszertani kategóriába tartozó mikroorganizmus kimutatása a betegből önmagában még nem elegendő bizonyíték arra, hogy a betegségnek valóban az a kórokozója. Igazolni kell a továbbiakban, hogy rendelkezik azokkal a virulenciafaktoroknak nevezett tényezőkkel, amelyek valójában képessé teszik az adott betegség kiváltására. Robert Koch idejében csak fenotípusos ismeretek álltak rendelkezésre, azok figyelembevételével fogalmazta meg posztulátumait. Később, a megszerzett molekuláris ismeretek birtokában, a posztulátumokat molekuláris szinten is értelmezték. A beteg személyét biológiai, szociális és pszichés egységként kezelő holisztikus megközelítésnek is eleget téve, a posztulátumokat a kórokozó mellett az esetben érintett gazdaszervezet egyedi tulajdonságainak figyelembevételével tovább szélesítették. A dolgozat a fenti kérdéseket példákkal illusztrálva tárgyalja, majd kitér a gyakorlati hasznosítás lehetőségeire. Orv Hetil. 2021; 162(50): 1991–1999. Summary. Efforts to explore the casual background of infectious diseases have been ambitioned for a long time. Terms and requirements (postulates) have been created to describe in which case a microorganism can be regarded as a causative agent of a given infectious disease. Demonstration of a representative of a pathogenic taxonomic category in the patient, however, does not prove its causative role in itself. It should also be verified if the microbe possesses the so-called virulence factors enabling it to trigger the given disease. At the time when Robert Koch formulated his postulates, only phenotypic characters were at his disposal. Later, in possession of a substantial genetic knowledge, the postulates have been adapted to molecular level. For having a holistic approach, the postulates have been extended also to the host’s individual biological, social and psychological attributes. This paper discusses the above issues with examples for illustration, and outlines their practical applicabilities. Orv Hetil. 2021; 162(50): 1991–1999.

Bringing order to a complex system: phenotypic and genotypic evidence contribute to the taxonomy of Tityus (Scorpiones, Buthidae) and support the description of a new species

We present a molecular phylogenetic analysis including a survey for overlooked phenotypic characters. Based on both analysis and characters a new cave-dwelling species is described: Tityus (Tityus) spelaeussp. nov. from the Russão II cave, Posse, state of Goiás, Central Brazil. Characters such as the glandular regions of the female pectinal basal piece and basal middle lamellae of pectines, and the distribution of the ventral setae of telotarsi I–IV proved to be useful to constructing the taxonomy of species and species groups of Tityus. The new species is a member of the Tityus trivittatus species-group of Tityus (Tityus) and can be readily recognized by the immaculate coloration pattern and the more developed glandular region on the female pectinal basal piece. In addition, we provide a discussion of the phylogenetic relationships observed within Tityus, on the relevance of the new phenotypic characters to the modern taxonomy of the genus Tityus, and to the records of Brazilian cave scorpions.

Nutritional Metabolites of Red Pigmented Lettuce (Lactuca sativa) Germplasm and Correlations with Selected Phenotypic Characters

Lettuce is an important dietary source of bioactive phytochemicals. Screening and identification of the health beneficial metabolites and evaluating the relationships with phenotypic characters can help consumers adjust their preferences for lettuce plant types. Thus, we explored the major health-beneficial individual metabolites and antioxidant potential of 113 red pigmented lettuce leaf samples. A UV–Vis spectrophotometer and UPLC-DAD-QTOF/MS (TQ/MS) instruments were used for the identification and quantification of metabolites and antioxidant activity accordingly. The metabolites were quantified against their corresponding external standards. The contents of metabolites varied significantly among lettuce samples. Cyanidin 3-O-(6″-O-malonyl)glucoside (4.7~5013.6 μg/g DW), 2,3-di-O-caffeoyltartaric acid (337.1~19,957.2 μg/g DW), and quercetin 3-O-(6″-O-malonyl)glucoside (45.4~31,121.0 μg/g DW) were the most dominant in red pigmented lettuce samples among anthocyanins, hydroxycinnamoyl derivatives, and flavonols, respectively. Lettuces with dark and very dark red pigmented leaves, circular leaf shape, a strong degree of leaf undulation, and highly dense leaf incisions were found to have high levels of flavonoids and hydroxycinnamoyl derivatives. Principal component analysis was used to investigate similarities and/or differences between samples, and the partial least square discriminant analysis classified them into known groups. The key variables that contributed highly were determined. Our report provides critical data on the bioactive constituents of red pigmented lettuce to breeders developing varieties with enhanced bioactive compounds and to nutraceutical companies developing nutrient dense foods and pharmaceutical formulations.

Leaf Blight Caused by Colletotrichum Fructicola of Large Cardamom (Amomum Subulatam Roxb.) an Important Cash Crop Grown in Sikkim, India

Large cardamom (Amommum subulatum Roxb.) a high valued spice crop grown in Sikkim Himalaya is now facing a devastating leaf blight disease that has brought down the yield drastically. Present study was focused on identification of this major fungal pathogen based on the morphological and molecular characterization. During this study infected leaves of large cardamom with blighted appearance were collected from all the four districts of Sikkim. The pathogen was isolated using Potato Dextrose Agar (PDA) medium, incubated at 25°C. The mycelium was septate, hyaline, and 2-4 µm wide. The conidiospores were cylindrical with both ends rounded, sometimes oblong. Length and breadth were 11-12 µm and 3-4 µm, respectively. On the basis colony morphology, growth and microscopic observations, out of the total 48 samples studied Colletotrichum sp. was identified from 14 samples. Based on phylogenetic analysis of the ITS4, ITS5 and ApMAT genes and phenotypic characters (colony morphology, microscopic features) the isolate (No. LC05) isolated from the sample collected from the village Assam Linzey, East Sikkim showed 100% homology with Colletotrichum fructicola from NCBI database. The pathogenicity of C. fructicola was also confirmed during the study. The fungal culture has been deposited at the NFCCI-ARI, Pune with an accession number NFCCI 4542 and the sequences have been deposited in NCBI GenBank with accession number (ITS) MN710587, (ApMAT) MW348934 respectively. To the best of our knowledge this is the first report of C. fructicola causing blight disease of large cardamom. Also the finding is very important to improve the disease control strategies of this high valued cash crop.