scholarly journals Molecular Characterization and Phylogenetic Relationships of Melon (Cucumis melo L. Meloni Cultivar) Based on Expression of Andromonoecious Gene

2020 ◽  
Vol 27 (2) ◽  
pp. 174
Author(s):  
Eka Wasi’ Al-Mughni ◽  
Budi Setiadi Daryono
Keyword(s):  
Gene Expression ◽  
Phylogenetic Analysis ◽  
Phylogenetic Tree ◽  
Molecular Characterization ◽  
Phylogenetic Relationships ◽  
Cucumis Melo ◽  
Specific Primer ◽  
Economic Prospect ◽  
Cucumis Melo L

Melon (Cucumis melo L.) is one of horticultural commodities with good economic prospect for melon farmers because of several advantages. 'Meloni' is result of crossing between ‘Sun Lady-3’ and ‘PI 371795’ by Genetics and Breeding Laboratory, Faculty of Biology, UGM. The 'Meloni' plant produces females flowers that expressed by CmACS-7 gene and it can be expected that melon plants 'Meloni' included in the primitive cultivars, while 'Meloni' is a cultivation cultivar. The aim of this research is to study the relation of 'Meloni' kinship to primitive and modern cultivars. Characterization of the CmACS-7 gene is begun by a total isolation process of RNA from a sample of melon plant flowers and detection using a specific primer. The cDNA bands obtained are sequenced and then phylogenetic analysis is performed based on CmACS-7 gene expression using MEGA 7.0. Result of this study show that the presence of the CmACS-7 gene in female and hermaphrodite flowers of ‘Meloni’, ‘PI 371795’, ‘Sun Lady-3’, and ‘Hikapel’. Phylogenetic tree analyze show that ‘Meloni’ from female plant, ‘PI 371795’ and ‘Sun Lady-3’ have closely relation, while 'Hikapel' separated from other cultivars.

Molecular characterization of small and medium segments of Crimean-Congo hemorrhagic fever virus in Turkey

2020 ◽  
Vol 15 (4) ◽  
pp. 247-254
Author(s):  
Murat Karamese ◽  
Erkan Ozmen ◽  
Hakan Aydin ◽  
Mehmet Ozkan Timurkan ◽  
Mesud Fakirullahoglu
Keyword(s):  
Infectious Disease ◽  
Phylogenetic Analysis ◽  
Phylogenetic Tree ◽  
Molecular Characterization ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Relationship Of

Aim: The objective was to investigate the genotypic relationship of S and M segments in Crimean-Congo hemorrhagic fever virus (CCHFV) by phylogenetic analysis in 25 patients from seven endemic cities in Turkey. Materials & methods: A total of 25 samples from patients with CCHF were included between 2012 and 2015. Phylogenetic tree analyses were inferred using MEGA version-6.0 and distances were calculated by Kimura’s 2-parameter. Results: Phylogenetic analysis showed that all isolated viruses (n = 25) were in the predicted clades such as clade V- Europe-1 regarding both S and M segments of the CCHFV. Conclusion: Further epidemiological, molecular and phylogenic studies should be performed in both reservoir animals/vectors and humans to determine the incidence of tick-borne infectious disease and to help to develop vaccines for prevention of the disease.

Molecular characterization of South and East Asian melon, Cucumis melo L., and the origin of Group Conomon var. makuwa and var. conomon revealed by RAPD analysis

Euphytica ◽  
2006 ◽  
Vol 153 (1-2) ◽  
pp. 233-247 ◽  
Author(s):  
Katsunori Tanaka ◽  
Atsushi Nishitani ◽  
Yukari Akashi ◽  
Yoshiteru Sakata ◽  
Hidetaka Nishida ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Cucumis Melo ◽  
Rapd Analysis ◽  
East Asian ◽  
Cucumis Melo L

Morphological, histological and molecular characterization of Myxidium cf. rhodei infecting the kidney of Rutilus rutilus

2020 ◽  
Vol 141 ◽  
pp. 39-46
Author(s):  
MD Dorjievna Batueva ◽  
X Pan ◽  
J Zhang ◽  
X Liu ◽  
W Wei ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Rutilus Rutilus ◽  
Longitudinal Axis ◽  
Suture Line ◽  
Present Species ◽  
Supplementary Data

In the present study, we provide supplementary data for Myxidium cf. rhodei Léger, 1905 based on morphological, histological and molecular characterization. M. cf. rhodei was observed in the kidneys of 918 out of 942 (97%) roach Rutilus rutilus (Linnaeus, 1758). Myxospores of M. cf. rhodei were fusiform with pointed ends, measuring 12.7 ± 0.1 SD (11.8-13.4) µm in length and 4.6 ± 0.1 (3.8-5.4) µm in width. Two similar pear-shaped polar capsules were positioned at either ends of the longitudinal axis of the myxospore: each of these capsules measured 4.0 ± 0.1 (3.1-4.7) µm in length and 2.8 ± 0.1 (2.0-4.0) µm in width. Polar filaments were coiled into 4 to 5 turns. Approximately 18-20 longitudinal straight ridges were observed on the myxospore surface. The suture line was straight and distinctive, running near the middle of the valves. Histologically, the plasmodia of the present species were found in the Bowman’s capsules, and rarely in the interstitium of the host. Phylogenetic analysis revealed that M. cf. rhodei was sister to M. anatidum in the Myxidium clade including most Myxidium species from freshwater hosts.

scholarly journals Gene Expression Profiling and Molecular Characterization of Antimony Resistance in Leishmania amazonensis

2011 ◽  
Vol 5 (5) ◽  
pp. e1167 ◽  
Author(s):  
Rubens L. do Monte-Neto ◽  
Adriano C. Coelho ◽  
Frédéric Raymond ◽  
Danielle Légaré ◽  
Jacques Corbeil ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Molecular Characterization ◽  
Expression Profiling ◽  
Leishmania Amazonensis ◽  
Antimony Resistance

scholarly journals A morphological and molecular characterization of vine mealybug populations (Hemiptera, Pseudococcidae) from Tunisia

2012 ◽  
Vol 44 (1) ◽  
pp. 5 ◽  
Author(s):  
Ramzi Mansour ◽  
Vincenzo Cavalieri ◽  
Gaetana Mazzeo ◽  
Kaouthar Grissa Lebdi ◽  
Agatino Russo
Keyword(s):  
Phylogenetic Tree ◽  
Molecular Characterization ◽  
Adult Female ◽  
Morphological Examination ◽  
Molecular Analyses ◽  
Planococcus Ficus ◽  
Morphological Studies ◽  
Vine Mealybug ◽  
Morphological And Molecular Characterization

Some vine mealybug, <em>Planococcus ficus </em>(Signoret) populations in Tunisian vineyards have been morphologically and genetically characterized. The morphological examination was based on the main distinctive characteristics of species of <em>Planococcus</em>, namely the number and distribution of the multilocular disc pores and tubular ducts on the adult female. This showed the existence of two different vine mealybug populations in Tunisia. Likewise, in the molecular analyses, two separate clades were revealed in the neighbour-joining phylogenetic tree, supporting the morphological studies and suggesting that there are two distinct populations of <em>P. ficus</em> on grapevine in Tunisia.

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