Local Cerebral Blood Flow Responses to Direct Stimulation in Somatosensory Cortex: Laser Doppler Flowmetric and Autoradiographic Analysis

2006 ◽  
Author(s):  
Zhongchi Luo ◽  
Jasbeer Dhawan ◽  
Helene Benveniste ◽  
Mei Yu ◽  
Andrew Gifford ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Somatosensory Cortex ◽  
Laser Doppler ◽  
Local Cerebral Blood Flow ◽  
Direct Stimulation ◽  
Autoradiographic Analysis

scholarly journals A Cerebral Vasoconstrictive Effect of Some Adenosine Analogues. A Study of Adenosine Analogues on Local Cerebral Blood Flow and Glucose Utilisation in the Rat

1989 ◽  
Vol 9 (4) ◽  
pp. 548-555 ◽  
Author(s):  
Douglas E. McBean ◽  
John J. Grome ◽  
A. Murray Harper
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Mechanism Of Action ◽  
Brain Regions ◽  
Laser Doppler ◽  
Metabolic Demand ◽  
Local Cerebral Blood Flow ◽  
Vascular Effects ◽  
Adenosine Analogues ◽  
Glucose Utilisation

Local CBF (LCBF) in the rat was determined using [14C]iodoantipyrine autoradiography. Adenosine and 5'-( N-ethyl)carboxamidoadenosine in a 15-min infusion had no significant effect on LCBF, although there was a tendency to increase. N6-Cyclohexyladenosine (CHA) and 2-chloroadenosine (2-CADO) significantly decreased LCBF in a number of brain regions. Laser-Doppler experiments using CHA confirmed that CHA decreased CBF and that this change was monophasic. Further experiments involving the use of [14C]2-deoxyglucose autoradiography showed that the unexpected vascular effects of CHA and 2-CADO were not a consequence of a decreased metabolic demand. The available data do not allow us to identify the mechanism of action by which the known vasodilators CHA and 2-CADO were able to cause a vasoconstriction and a decrease in LCBF.

scholarly journals Autoradiographic Analysis on Second-Messenger Systems and Local Cerebral Blood Flow in Ischemic Gerbil Brain

1991 ◽  
Vol 11 (2) ◽  
pp. 283-291 ◽  
Author(s):  
Kortaro Tanaka ◽  
Fumio Gotoh ◽  
Shintaro Gomi ◽  
Shutaro Takashima ◽  
Ban Mihara
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Second Messenger ◽  
Brain Regions ◽  
Local Cerebral Blood Flow ◽  
Second Messenger Systems ◽  
Autoradiographic Analysis ◽  
Autoradiographic Technique ◽  
The Right ◽  
Pdbu Binding

Alterations of the second-messenger systems, adenylate cyclase (AC) and protein kinase C (PKC), and local cerebral blood flow (1CBF) were evaluated during experimental cerebral ischemia in gerbils employing a quantitative autoradiographic method, which permitted these three parameters to be measured in the same brain. Ischemia was induced by occlusion of the right common carotid artery for 6 h. Animals attaining more than 5 in their ischemic scores were utilized for further experiments. At the end of ischemia, 1CBF was measured by the [14C]iodoantipyrine method. The AC and PKC activities were estimated by the autoradiographic technique developed in our laboratory using [3H]forskolin (FK) and [3H]phorbol-12,13-dibutyrate (PDBu), respectively. The 1CBF fell below 10 ml/100 g/min in most cerebral regions on the ligated side. The greatest reduction in FK binding was noted in the olfactory tubercle, caudate-putamen, and globus pallidus, followed by the hippocampus and cerebral cortices. The FK binding tended to be low at 1CBF < 20 ml/100 g/min in the cerebral cortices. However, the PDBu binding was relatively well preserved in each cerebral structure, and no significant correlation between 1CBF and PDBu binding was noted in the cerebral cortices. The AC system may thus be vulnerable to ischemic insult over extensive brain regions, while the PKC system may be relatively resistant to ischemia.

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