scholarly journals A Cerebral Vasoconstrictive Effect of Some Adenosine Analogues. A Study of Adenosine Analogues on Local Cerebral Blood Flow and Glucose Utilisation in the Rat

1989 ◽  
Vol 9 (4) ◽  
pp. 548-555 ◽  
Author(s):  
Douglas E. McBean ◽  
John J. Grome ◽  
A. Murray Harper
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Mechanism Of Action ◽  
Brain Regions ◽  
Laser Doppler ◽  
Metabolic Demand ◽  
Local Cerebral Blood Flow ◽  
Vascular Effects ◽  
Adenosine Analogues ◽  
Glucose Utilisation

Local CBF (LCBF) in the rat was determined using [14C]iodoantipyrine autoradiography. Adenosine and 5'-( N-ethyl)carboxamidoadenosine in a 15-min infusion had no significant effect on LCBF, although there was a tendency to increase. N6-Cyclohexyladenosine (CHA) and 2-chloroadenosine (2-CADO) significantly decreased LCBF in a number of brain regions. Laser-Doppler experiments using CHA confirmed that CHA decreased CBF and that this change was monophasic. Further experiments involving the use of [14C]2-deoxyglucose autoradiography showed that the unexpected vascular effects of CHA and 2-CADO were not a consequence of a decreased metabolic demand. The available data do not allow us to identify the mechanism of action by which the known vasodilators CHA and 2-CADO were able to cause a vasoconstriction and a decrease in LCBF.

scholarly journals Postnatal Changes in Local Cerebral Blood Flow Measured by the Quantitative Autoradiographic [14C]Iodoantipyrine Technique in Freely Moving Rats

1989 ◽  
Vol 9 (5) ◽  
pp. 579-588 ◽  
Author(s):  
Astrid Nehlig ◽  
Anne Pereira de Vasconcelos ◽  
Sylvette Boyet
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Glucose Utilization ◽  
Brain Regions ◽  
Postnatal Period ◽  
High Rate ◽  
Local Cerebral Blood Flow ◽  
Adult Rat ◽  
Freely Moving Rats ◽  
Freely Moving

The postnatal changes in local cerebral blood flow in freely moving rats were measured by means of the quantitative autoradiographic [14C]iodoantipyrine method. The animals were studied at 10, 14, 17, 21, and 35 days and at the adult stage. At 10 days after birth, rates of blood flow were very low and quite homogeneous in most cerebral structures except in a few posterior areas. From these relatively uniform levels, values of local cerebral blood flow rose notably to reach a peak at 17 days in all brain regions studied. Rates of blood flow decreased between 17 and 21 days after birth and then increased from weaning time to reach the known characteristic distribution of the adult rat. The postnatal evolution of local cerebral blood flow in the rat is in good agreement with previous studies in other species such as dog and humans that also show higher rates of cerebral blood flow and glucose utilization at immature stages. However, in the rat, local cerebral blood flow and local cerebral glucose utilization are not coupled over the whole postnatal period studied, since blood flow rates reach peak values at 17 days whereas glucose utilization remains still quite low at that stage. The high rate of cerebral blood flow in the 17-day-old rat may reflect the energetic and biosynthetic needs of the actively developing brain that are completed by the summation of glucose and ketone body utilization.

Acute cocaine administation: effects on local cerebral blood flow and metabolic demand in the rat

1991 ◽  
Vol 548 (1-2) ◽  
pp. 310-314 ◽  
Author(s):  
John Sharkey ◽  
Douglas E. McBean ◽  
Paul A.T. Kelly
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Metabolic Demand ◽  
Local Cerebral Blood Flow

scholarly journals Local Cerebral Blood Flow in Rats with Congenital Hydrocephalus

1993 ◽  
Vol 13 (3) ◽  
pp. 531-534 ◽  
Author(s):  
H. C. Jones ◽  
H. K. Richards ◽  
R. M. Bucknall ◽  
J. D. Pickard
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Inferior Colliculus ◽  
Cerebellar Cortex ◽  
Matched Control ◽  
Brain Regions ◽  
Quantitative Autoradiography ◽  
Congenital Hydrocephalus ◽  
Local Cerebral Blood Flow ◽  
Cortical Regions

Local cerebral blood flow (LCBF) has been measured by [14C]iodoantipyrine quantitative autoradiography in H-Tx rats with inherited congenital hydrocephalus at 10, 21, and 30 days after birth. LCBF at 10 days was uniformly low in all ten brain regions studied and not significantly different between hydrocephalic and age-matched control rats. By 21 days LCBF had increased significantly in control rats. LCBF was significantly lower (<69%) in the hydrocephalics in all cortical regions and in the inferior colliculus LCBF at both 21 and 30 days. The cerebellar cortex, pons, and caudate were not significantly affected. At 30 days LCBF ranged from 55 to 115 ml 100 g−1 min−1 for hydrocephalics and from 100 to 183 ml 100 g−1 min−1 for controls.

Local Cerebral Blood Flow, Local Cerebral Glucose Utilization, and Flow-Metabolism Coupling during Sevoflurane versus Isoflurane Anesthesia in Rats 

1998 ◽  
Vol 89 (6) ◽  
pp. 1480-1488 ◽  
Author(s):  
Christian Lenz ◽  
Annette Rebel ◽  
Klaus van Ackern ◽  
Wolfgang Kuschinsky ◽  
Klaus F. Waschke
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Glucose Utilization ◽  
Brain Regions ◽  
Local Analysis ◽  
Sprague Dawley ◽  
Local Cerebral Blood Flow ◽  
Local Cerebral Glucose Utilization ◽  
Isoflurane Anesthesia ◽  
Cerebral Glucose Utilization

Background Compared to isoflurane, knowledge of local cerebral glucose utilization (LCGU) and local cerebral blood flow (LCBF) during sevoflurane anesthesia is limited. Methods LCGU, LCBF, and their overall means were measured in Sprague-Dawley rats (8 groups, n=6 each) during sevoflurane and isoflurane anesthesia, 1 and 2 MAC, and in conscious control animals (2 groups, n=6 each) using the autoradiographic 2-[14C]deoxy-D-glucose and 4-iodo-N-methyl-[14C]antipyrine methods. Results During anesthesia, mean cerebral glucose utilization was decreased: control, 56+/-5 micronmol x 100 g(-1) x min(-1); 1 MAC isoflurane, 32+/-4 micromol x 100 g(-1) x min(-1) (-43%); 1 MAC sevoflurane, 37+/-5 micromol x 100 g(-1) x min(-1) (-34%); 2 MAC isoflurane, 23+/-3 micromol x 100 g(-1) x min(-1) (-58%); 2 MAC sevoflurane, 23+/-5 micromol x 100 g(-1) x min(-1) (-59%). Local analysis showed a reduction in LCGU in the majority of the 40 brain regions analyzed. Mean cerebral blood flow was increased as follows: control 93+/-8 ml x 100 g(-1) x min(-1); 1 MAC isofurane, 119+/-19 ml x 100 g(-1) x min(-1) (+28%); 1 MAC sevoflurane, 104+/-15 ml x 100 g(-1) x min(-1) (+12%); 2 MAC isoflurane, 149+/-17 ml x 100 g(-1) x min(-1) (+60%); 2 MAC sevoflurane, 118+/-21 ml x 100 g(-1) min(-1) (+27%). LCBF was increased in most brain structures investigated. Correlation coefficients obtained for the relationship between LCGU and LCBF were as follows: control 0.93; 1 MAC isoflurane, 0.89; 2 MAC isoflurane, 0.71; 1 MAC sevoflurane, 0.83; 2 MAC sevoflurane, 0.59). Conclusion Mean and local cerebral blood flows were lower during sevoflurane than during isoflurane anesthesia. This difference cannot be explained by differing changes in glucose utilization because glucose utilization was decreased to the same extent in both groups.

Effects of Xenon on Cerebral Blood Flow and Cerebral Glucose Utilization in Rats

2001 ◽  
Vol 94 (2) ◽  
pp. 290-297 ◽  
Author(s):  
Thomas Frietsch ◽  
Ralph Bogdanski ◽  
Manfred Blobner ◽  
Christian Werner ◽  
Wolfgang Kuschinsky ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Steady State ◽  
Glucose Utilization ◽  
Brain Structures ◽  
Brain Regions ◽  
Local Cerebral Blood Flow ◽  
Mean Values ◽  
Cerebral Glucose Utilization ◽  
Local Brain

Background The effects of xenon inhalation on mean and local cerebral blood flow (CBF) and mean and local cerebral glucose utilization (CGU) were investigated using iodo-[14C]antipyrine and [14C]deoxyglucose autoradiography. Methods Rats were randomly assigned to the following groups: conscious controls (n = 12); 30% (n = 12) or 70% xenon (n = 12) for 45 min for the measurement of local CBF and CGU; or 70% xenon for 2 min (n = 6) or 5 min (n = 6) for the measurement of local CBF only. Results Compared with conscious controls, steady state inhalation of 30 or 70% xenon did not result in changes of either local or mean CBF. However, mean CBF increased by 48 and 37% after 2 and 5 min of 70% xenon short inhalation, which was entirely caused by an increased local CBF in cortical brain regions. Mean CGU determined during steady state 30 or 70% xenon inhalation remained unchanged, although local CGU decreased in 7 (30% xenon) and 18 (70% xenon) of the 40 examined brain regions. The correlation between CBF and CGU in 40 local brain structures was maintained during steady state inhalation of both 30 and 70% xenon inhalation, although at an increased slope at 70% xenon. Conclusion Effects of 70% xenon inhalation on CBF in rats are time-dependent. During steady state xenon inhalation (45 min), mean values of CBF and CGU do not differ from control values, and the relation of regional CBF to CGU is maintained, although reset at a higher level.

Acute methylenedioxymethamphetamine administration: effects on local cerebral blood flow and glucose utilisation in the dark agouti rat

2004 ◽  
Vol 173 (3-4) ◽  
pp. 287-295 ◽  
Author(s):  
Linda Quate ◽  
Douglas E. McBean ◽  
Isobel M. Ritchie ◽  
Henry J. Olverman ◽  
Paul A. T. Kelly
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Dark Agouti ◽  
Local Cerebral Blood Flow ◽  
Glucose Utilisation
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