In vivo Shortwave Infrared (SWIR) Confocal Fluorescence Imaging of Deep Mouse Brain with a Single-Photon Superconducting Nanowire Detector

2021 ◽  
Author(s):  
Fei Xia ◽  
Chris Xu
Keyword(s):  
Fluorescence Imaging ◽  
Mouse Brain ◽  
Single Photon ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging ◽  
Shortwave Infrared ◽  
Superconducting Nanowire

scholarly journals Non-Invasive Confocal Fluorescence Imaging of Mice Beyond 1700 nm Using Superconducting Nanowire Single-Photon Detectors

2021 ◽  
Author(s):  
Feifei Wang ◽  
Fuqiang Ren ◽  
Zhuoran Ma ◽  
Liangqiong Qu ◽  
Ronan Gourgues ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Fluorescence Imaging ◽  
Single Photon ◽  
Photon Detectors ◽  
Non Invasive ◽  
Single Photon Detectors ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging ◽  
Superconducting Nanowire

Light scattering by biological tissues sets a limit to the penetration depth of high-resolution optical microscopy imaging of live mammals in vivo. An effective approach to reduce light scattering and increase imaging depth is by extending the excitation and emission wavelengths to the > 1000 nm second near-infrared (NIR-II), also called the short-wavelength infrared (SWIR) window. Here, we developed biocompatible core-shell lead sulfide/cadmium sulfide (PbS/CdS) quantum dots emitting at ~1880 nm and superconducting nanowire single photon detectors (SNSPD) for single-photon detection up to 2000 nm, enabling one-photon fluorescence imaging window in the 1700-2000 nm (NIR-IIc) range. Confocal fluorescence imaging in NIR-IIc reached an imaging depth of ~ 800 μm through intact mouse head, and enabled non-invasive imaging of inguinal lymph nodes (LNs) without any surgery. In vivo molecular imaging of high endothelial venules (HEVs) with diameter down to ~ 6.6 μm in the lymph nodes was achieved, opening the possibility of non-invasive imaging of immune trafficking in lymph nodes at the single-cell/vessel level longitudinally.

scholarly journals In vivo hyperspectral confocal fluorescence imaging to determine pigment localization and distribution in cyanobacterial cells

2008 ◽  
Vol 105 (10) ◽  
pp. 4050-4055 ◽  
Author(s):  
W. F. J. Vermaas ◽  
J. A. Timlin ◽  
H. D. T. Jones ◽  
M. B. Sinclair ◽  
L. T. Nieman ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging

Real-Time In Vivo Confocal Fluorescence Imaging of Prostate Cancer Bone-Marrow Micrometastasis Development at the Cellular Level in Nude Mice

2016 ◽  
Vol 117 (11) ◽  
pp. 2533-2537 ◽  
Author(s):  
Shinji Miwa ◽  
Makoto Toneri ◽  
Kentaro Igarashi ◽  
Shuya Yano ◽  
Hiroaki Kimura ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Bone Marrow ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Nude Mice ◽  
Cellular Level ◽  
Confocal Fluorescence ◽  
Bone Marrow Micrometastasis ◽  
Confocal Fluorescence Imaging

scholarly journals In vivo confocal fluorescence imaging of skin surface cellular morphology

2002 ◽  
Vol 8 (S02) ◽  
pp. 1062-1063
Author(s):  
Daniel Thorn Leeson ◽  
C. Lynn Meyers ◽  
Kumar Subramanyan
Keyword(s):  
Fluorescence Imaging ◽  
Skin Surface ◽  
Cellular Morphology ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging
Sign in / Sign up

Export Citation Format

Share Document