Fluorescence lifetime imaging microscopy (FLIM) on the single molecule level

2001 ◽  
Author(s):  
Phillip Tinnefeld ◽  
V. Buschmann ◽  
D.-P. Herten ◽  
J.-P. Knemeyer ◽  
M. Sauer
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Single Molecule Level ◽  
Lifetime Imaging

Confocal Fluorescence Lifetime Imaging Microscopy (FLIM) at the Single Molecule Level

2000 ◽  
Vol 1 (3) ◽  
pp. 215-223 ◽  
Author(s):  
Philip Tinnefeld ◽  
Volker Buschmann ◽  
Dirk-Peter Herten ◽  
Kyung-Tae Han ◽  
Markus Sauer
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Single Molecule Level ◽  
Lifetime Imaging ◽  
Confocal Fluorescence

scholarly journals In situ real-time monitoring the mechanism of self-assembly of short peptide supramolecular polymers

2021 ◽  
Author(s):  
Mari C. Mañas-Torres ◽  
Cristina Gila-Vilchez ◽  
Juan Antonio Gonzalez Vera ◽  
Francisco Conejero-Lara ◽  
Victor Blanco ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Self Assembly ◽  
Correlation Spectroscopy ◽  
Supramolecular Polymers ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Single Molecule Fluorescence ◽  
Lifetime Imaging

Making use of the combination of multiparametric Fluorescence Lifetime Imaging Microscopy (FLIM) and single-molecule Fluorescence Lifetime Correlation Spectroscopy (FLCS), we have been able to study early stages of Fluorenylmethyloxycarbonyl-diphenylalanine (Fmoc-FF)...

Visualization of Cutaneous Distribution of Minocycline of a Topical Gel in Human Facial Skin with Two-Photon Excited Fluorescence Lifetime Imaging Microscopy (FLIM) and Phasor Analysis

2017 ◽  
Vol 1 ◽  
pp. s57
Author(s):  
Sinyoung Jeong ◽  
Maiko Hermsmeier ◽  
Sam Osseiran ◽  
Akira Yamamoto ◽  
Usha Nagavarapu ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Facial Skin ◽  
Topical Gel ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Phasor Analysis

Abstract Not AvailableStudy supported by BioPharmX.

Effects of Photon Losses on Fluorescence Lifetime Imaging Microscopy (FLIM) System Optimization

2015 ◽  
Vol 4 (2) ◽  
pp. 67-77
Author(s):  
Lior Turgeman ◽  
Tsviya Nayhoz ◽  
Nir Roth ◽  
Gilad Yahav ◽  
Avraham Hirshberg ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
System Optimization ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

P‐103: Non‐Destructive FMM Shadow‐Level Evaluation with Fluorescence Lifetime Imaging Microscopy

2021 ◽  
Vol 52 (1) ◽  
pp. 1466-1469
Author(s):  
Ki Young Yeon ◽  
Woo Young Lee ◽  
Junghan Shin ◽  
Young Gil Park ◽  
Nari Ahn
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Non Destructive

scholarly journals Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

2016 ◽  
Vol 52 (24) ◽  
pp. 4458-4461 ◽  
Author(s):  
Hideki Itoh ◽  
Satoshi Arai ◽  
Thankiah Sudhaharan ◽  
Sung-Chan Lee ◽  
Young-Tae Chang ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Heat Production ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
C2c12 Myotube

FLIM of ER thermo yellow and non-targeted mCherry reveals the Ca2+-dependent heat production localized to SR in C2C12 myotube.

scholarly journals Lifetime estimation of moving subcellular objects in frequency-domain fluorescence lifetime imaging microscopy

2015 ◽  
Vol 32 (10) ◽  
pp. 1821 ◽  
Author(s):  
Philippe Roudot ◽  
Charles Kervrann ◽  
Cedric M. Blouin ◽  
Francois Waharte
Keyword(s):  
Frequency Domain ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Estimation ◽  
Lifetime Imaging

Monitor RNA synthesis in live cell nuclei by using two-photon excited fluorescence lifetime imaging microscopy

2015 ◽  
Author(s):  
Xiao Peng ◽  
Danying Lin ◽  
Yan Wang ◽  
Jing Qi ◽  
Wei Yan ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Rna Synthesis ◽  
Live Cell ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Cell Nuclei ◽  
Two Photon ◽  
Lifetime Imaging
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