Fluorescence lifetime imaging microscopy (FLIM) on the single molecule level

2001 ◽  
Author(s):  
Phillip Tinnefeld ◽  
V. Buschmann ◽  
D.-P. Herten ◽  
J.-P. Knemeyer ◽  
M. Sauer
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Single Molecule Level ◽  
Lifetime Imaging

scholarly journals In situ real-time monitoring the mechanism of self-assembly of short peptide supramolecular polymers

2021 ◽  
Author(s):  
Mari C. Mañas-Torres ◽  
Cristina Gila-Vilchez ◽  
Juan Antonio Gonzalez Vera ◽  
Francisco Conejero-Lara ◽  
Victor Blanco ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Self Assembly ◽  
Correlation Spectroscopy ◽  
Supramolecular Polymers ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Single Molecule Fluorescence ◽  
Lifetime Imaging

Making use of the combination of multiparametric Fluorescence Lifetime Imaging Microscopy (FLIM) and single-molecule Fluorescence Lifetime Correlation Spectroscopy (FLCS), we have been able to study early stages of Fluorenylmethyloxycarbonyl-diphenylalanine (Fmoc-FF)...

scholarly journals Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

2016 ◽  
Vol 52 (24) ◽  
pp. 4458-4461 ◽  
Author(s):  
Hideki Itoh ◽  
Satoshi Arai ◽  
Thankiah Sudhaharan ◽  
Sung-Chan Lee ◽  
Young-Tae Chang ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Heat Production ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
C2c12 Myotube

FLIM of ER thermo yellow and non-targeted mCherry reveals the Ca2+-dependent heat production localized to SR in C2C12 myotube.

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