By using a new kind of single-beam normalized photo-acoustic tomography spectroscopy (PAS-CT) technology with non-damage detection, the photo-acoustic tomography spectroscopy and optical absorption properties of green and yellow leaves ofBauhinia blakeanawere studied. The results show that: the photo-acoustic tomography spectroscopy ofBauhinia blakeanaleaves could be obtained from different chopping frequency and different sample positions, and photosynthetic pigment content of their leaves is closely related to their photosynthetic intensity. The more photosynthetic pigment content the leaves ofBauhinia blakeanacontain, the bigger optical absorption coefficient and the higher photosynthetic efficiency they have. The photosynthetic pigment content of the green leaves ofBauhinia blakeanais higher than that of the yellow ones. As a result, the photosynthesis of green leaves is better than that of the yellow ones. According to the results of our research, the photosynthetic efficiency ofBauhinia blakeanacould be improved and its growth time could be effectively controlled to enhance the growth ofBauhinia blakeana. Furthermore, it could play an important role on the development of forestry, and meet the need of forest for 6 billion people. The research showed high science value to the study and applications of the photosynthesis of plants.