scholarly journals Localization of Perlecan and Heparanase in Hertwig's Epithelial Root Sheath During Root Formation in Mouse Molars

2006 ◽  
Vol 54 (10) ◽  
pp. 1105-1113 ◽  
Author(s):  
Azumi Hirata ◽  
Hiroaki Nakamura
Keyword(s):  
Root Formation ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath

Immortalized Hertwig's epithelial root sheath cell line works as model for epithelial–mesenchymal interaction during tooth root formation

2019 ◽  
Vol 235 (3) ◽  
pp. 2698-2709 ◽  
Author(s):  
Sicheng Zhang ◽  
Xuebing Li ◽  
Shikai Wang ◽  
Yan Yang ◽  
Weihua Guo ◽  
...  
Keyword(s):  
Cell Line ◽  
Root Formation ◽  
Tooth Root ◽  
Sheath Cell ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath

Developmental Properties of the Hertwig’s Epithelial Root Sheath in Mice

2004 ◽  
Vol 83 (9) ◽  
pp. 688-692 ◽  
Author(s):  
H. Yamamoto ◽  
S.-W. Cho ◽  
E.-J. Kim ◽  
J.-Y. Kim ◽  
N. Fujiwara ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Culture System ◽  
Root Formation ◽  
Expression Patterns ◽  
Tooth Root ◽  
Comprehensive Model ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath ◽  
In Vitro Culture System

Hertwig’s epithelial root sheath (HERS) plays an important role in tooth root formation. In this study, we examined root formation of the first molar in mice, focusing on cell proliferation, cell death, cell migration, and the expression patterns of the signaling molecules, including glycoproteins and proteoglycans between PN8 and PN26. The number of HERS cells decreased during root formation, although HERS retained total length until PN15. The migration of HERS cells did not occur during root formation. Moreover, the immunopositive reaction of laminin beta-3 and syndecan-1 in HERS indicates that both cell adhesion and cell proliferation are essential for HERS development. Bmp-2, Bmp-4, and Msx-2 were expressed in HERS cells during root formation. We also developed an in vitro culture system for investigating the periodontium and suggest that this system provides an excellent vehicle for full exploration, and hence improved understanding, of the development and regeneration of the periodontium. Together, our results provide a comprehensive model describing the morphogenesis of early root development in vertebrates.

scholarly journals Ameloblastin in Hertwig’s Epithelial Root Sheath Regulates Tooth Root Formation and Development

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e54449 ◽  
Author(s):  
Naoto Hirose ◽  
Atsushi Shimazu ◽  
Mineo Watanabe ◽  
Kotaro Tanimoto ◽  
Souichi Koyota ◽  
...  
Keyword(s):  
Root Formation ◽  
Tooth Root ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath

Local Irradiation of Mouse Tooth Germ Gives Insight into the Direct Effects of Irradiation on Root Development

2021 ◽  
Author(s):  
Yoshiaki Ide ◽  
Taka Nakahara ◽  
Tetsuya Fukada ◽  
Masanori Nasu
Keyword(s):  
Root Development ◽  
Root Formation ◽  
Tooth Germ ◽  
Lead Glass ◽  
Local Irradiation ◽  
Root Sheath ◽  
Calcified Tissue ◽  
Radiation Induced ◽  
Dentin Formation ◽  
Root Apices

To elucidate the mechanism underlying the failure of root formation after irradiation, we established a method of local irradiation of the molar tooth germ and demonstrated that radiation directly affected dental root development. In the current study, to locally irradiate the lower first molars of 5-day-old C57BL/6J mice, we used lead glass containing a hole as a collimator. We confirmed that our local irradiation method targeted only the tooth germ. The irradiated root was immature in terms of apical growth, and dentin formation was irregular along the outside of the root apices. Moreover, calcified tissue apically surrounded Hertwig's epithelial root sheath, which disappeared abnormally early. This method using a local irradiation experimental model will facilitate research into radiation-induced disorders of dental root formation.

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