scholarly journals Correction: Electrical Pulse Stimulation of Cultured Human Skeletal Muscle Cells as an In Vitro Model of Exercise

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
Author(s):  
NataÅ¡a Nikolić ◽  
Siril Skaret Bakke ◽  
Eili Tranheim Kase ◽  
Ida Rudberg ◽  
Ingeborg Flo Halle ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
In Vitro Model ◽  
Muscle Cells ◽  
Electrical Pulse Stimulation ◽  
Human Skeletal Muscle Cells ◽  
Vitro Model ◽  
Pulse Stimulation ◽  
Stimulation Of

scholarly journals Electrical Pulse Stimulation of Cultured Human Skeletal Muscle Cells as an In Vitro Model of Exercise

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33203 ◽  
Author(s):  
Nataša Nikolić ◽  
Siril Skaret Bakke ◽  
Eili Tranheim Kase ◽  
Ida Rudberg ◽  
Ingeborg Flo Halle ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
In Vitro Model ◽  
Muscle Cells ◽  
Electrical Pulse Stimulation ◽  
Human Skeletal Muscle Cells ◽  
Vitro Model ◽  
Pulse Stimulation ◽  
Stimulation Of

scholarly journals Effect of differentiation, de novo innervation, and electrical pulse stimulation on mRNA and protein expression of Na+,K+-ATPase, FXYD1, and FXYD5 in cultured human skeletal muscle cells

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0247377
Author(s):  
Vid Jan ◽  
Katarina Miš ◽  
Natasa Nikolic ◽  
Klemen Dolinar ◽  
Metka Petrič ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Human Skeletal Muscle ◽  
De Novo ◽  
Muscle Cells ◽  
Electrical Pulse ◽  
Electrical Pulse Stimulation ◽  
Human Skeletal Muscle Cells ◽  
Pulse Stimulation ◽  
Low Serum

Denervation reduces the abundance of Na+,K+-ATPase (NKA) in skeletal muscle, while reinnervation increases it. Primary human skeletal muscle cells, the most widely used model to study human skeletal muscle in vitro, are usually cultured as myoblasts or myotubes without neurons and typically do not contract spontaneously, which might affect their ability to express and regulate NKA. We determined how differentiation, de novo innervation, and electrical pulse stimulation affect expression of NKA (α and β) subunits and NKA regulators FXYD1 (phospholemman) and FXYD5 (dysadherin). Differentiation of myoblasts into myotubes under low serum conditions increased expression of myogenic markers CD56 (NCAM1), desmin, myosin heavy chains, dihydropyridine receptor subunit α1S, and SERCA2 as well as NKAα2 and FXYD1, while it decreased expression of FXYD5 mRNA. Myotubes, which were innervated de novo by motor neurons in co-culture with the embryonic rat spinal cord explants, started to contract spontaneously within 7–10 days. A short-term co-culture (10–11 days) promoted mRNA expression of myokines, such as IL-6, IL-7, IL-8, and IL-15, but did not affect mRNA expression of NKA, FXYDs, or myokines, such as musclin, cathepsin B, meteorin-like protein, or SPARC. A long-term co-culture (21 days) increased the protein abundance of NKAα1, NKAα2, FXYD1, and phospho-FXYD1Ser68 without attendant changes in mRNA levels. Suppression of neuromuscular transmission with α-bungarotoxin or tubocurarine for 24 h did not alter NKA or FXYD mRNA expression. Electrical pulse stimulation (48 h) of non-innervated myotubes promoted mRNA expression of NKAβ2, NKAβ3, FXYD1, and FXYD5. In conclusion, low serum concentration promotes NKAα2 and FXYD1 expression, while de novo innervation is not essential for upregulation of NKAα2 and FXYD1 mRNA in cultured myotubes. Finally, although innervation and EPS both stimulate contractions of myotubes, they exert distinct effects on the expression of NKA and FXYDs.

scholarly journals Engineering a 3D in vitro model of human skeletal muscle at the single fiber scale

PLoS ONE ◽  
2020 ◽  
Vol 15 (5) ◽  
pp. e0232081 ◽  
Author(s):  
Anna Urciuolo ◽  
Elena Serena ◽  
Rusha Ghua ◽  
Susi Zatti ◽  
Monica Giomo ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
In Vitro Model ◽  
Single Fiber ◽  
3D In Vitro Model ◽  
Vitro Model

scholarly journals Silk fibroin scaffolds with muscle-like elasticity support in vitro differentiation of human skeletal muscle cells

2016 ◽  
Vol 11 (11) ◽  
pp. 3178-3192 ◽  
Author(s):  
Vishal Chaturvedi ◽  
Deboki Naskar ◽  
Beverley F. Kinnear ◽  
Elizabeth Grenik ◽  
Danielle E. Dye ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Silk Fibroin ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
In Vitro Differentiation ◽  
Human Skeletal Muscle Cells

Electrical pulse stimulation of cultured skeletal muscle cells as a model forin vitroexercise - possibilities and limitations

2016 ◽  
Vol 220 (3) ◽  
pp. 310-331 ◽  
Author(s):  
N. Nikolić ◽  
S. W. Görgens ◽  
G. H. Thoresen ◽  
V. Aas ◽  
J. Eckel ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Electrical Pulse ◽  
Electrical Pulse Stimulation ◽  
Pulse Stimulation ◽  
Stimulation Of

Development of an in vitro model for study of the efficacy of ischemic preconditioning in human skeletal muscle against ischemia-reperfusion injury

2006 ◽  
Vol 101 (5) ◽  
pp. 1335-1342 ◽  
Author(s):  
Glyka Martou ◽  
Catherine A. O’Blenes ◽  
Ning Huang ◽  
Sandra E. McAllister ◽  
Peter C. Neligan ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protective Effect ◽  
Ischemic Preconditioning ◽  
Human Skeletal Muscle ◽  
In Vitro Model ◽  
Ischemia Reperfusion ◽  
Mtt Reduction ◽  
Vitro Model ◽  
Hypoxia Reoxygenation

Ischemia-reperfusion (I/R) injury causes skeletal muscle infarction and ischemic preconditioning (IPC) augments ischemic tolerance in animal models. To date, this has not been demonstrated in human skeletal muscle. This study aimed to develop an in vitro model to investigate the efficacy of simulated IPC in human skeletal muscle. Human skeletal muscle strips were equilibrated in oxygenated Krebs-Henseleit-HEPES buffer (37°C). Aerobic and reperfusion phases were simulated by normoxic incubation and reoxygenation, respectively. Ischemia was simulated by hypoxic incubation. Energy store, cell viability, and cellular injury were assessed using ATP, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2 H-tetrazolium bromide (MTT), and lactate dehydrogenase (LDH) assays, respectively. Morphological integrity was assessed using electron microscopy. Studies were designed to test stability of the preparation ( n = 5–11) under normoxic incubation over 24 h; the effect of 1, 2, 3, 4, or 6 h hypoxia followed by 2 h of reoxygenation; and the protective effect of hypoxic preconditioning (HPC; 5 min of hypoxia/5 min of reoxygenation) before 3 h of hypoxia/2 h of reoxygenation. Over 24 h of normoxic incubation, muscle strips remained physiologically intact as assessed by MTT, ATP, and LDH assays. After 3 h of hypoxia/2 h of reoxygenation, MTT reduction levels declined to 50.1 ± 5.5% ( P < 0.05). MTT reduction levels in HPC (82.3 ± 10.8%) and normoxic control (81.3 ± 10.2%) groups were similar and higher ( P < 0.05) than the 3 h of hypoxia/2 h of reoxygenation group (45.2 ± 5.8%). Ultrastructural morphology was preserved in normoxic and HPC groups but not in the hypoxia/reoxygenation group. This is the first study to characterize a stable in vitro model of human skeletal muscle and to demonstrate a protective effect of HPC in human skeletal muscle against hypoxia/reoxygenation-induced injury.

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