scholarly journals Silk fibroin scaffolds with muscle-like elasticity support in vitro differentiation of human skeletal muscle cells

2016 ◽  
Vol 11 (11) ◽  
pp. 3178-3192 ◽  
Author(s):  
Vishal Chaturvedi ◽  
Deboki Naskar ◽  
Beverley F. Kinnear ◽  
Elizabeth Grenik ◽  
Danielle E. Dye ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Silk Fibroin ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
In Vitro Differentiation ◽  
Human Skeletal Muscle Cells

scholarly journals Strawberry extract attenuates oxidative stress‐induced impaired insulin signaling in vitro in Human Skeletal Muscle Cells

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Krishnankutty Sandhya ◽  
Ravi Tadapaneni ◽  
Katie Banaszewski ◽  
Jack Cappozzo ◽  
Indika Edirisinghe ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Insulin Signaling ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Human Skeletal Muscle Cells ◽  
Impaired Insulin

scholarly journals Metabolic switching of human skeletal muscle cells in vitro

2011 ◽  
Vol 85 (5) ◽  
pp. 227-234 ◽  
Author(s):  
G.H. Thoresen ◽  
N.P. Hessvik ◽  
S.S. Bakke ◽  
V. Aas ◽  
A.C. Rustan
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Metabolic Switching ◽  
Human Skeletal Muscle Cells

scholarly journals Impact of Nucleoside Reverse Transcriptase Inhibitors on Mitochondrial DNA and RNA in Human Skeletal Muscle Cells

2008 ◽  
Vol 52 (8) ◽  
pp. 2825-2830 ◽  
Author(s):  
Akihiko Saitoh ◽  
Richard H. Haas ◽  
Robert K. Naviaux ◽  
Neurita G. Salva ◽  
Justine K. Wong ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mitochondrial Dna ◽  
Reverse Transcriptase ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Reverse Transcriptase Inhibitors ◽  
Human Skeletal Muscle Cells ◽  
Hiv 1

ABSTRACT We previously reported that 2′,3′-dideoxyinosine (didanosine, or ddI) significantly altered mitochondrial DNA (mtDNA) in peripheral blood mononuclear cells in human immunodeficiency virus type 1 (HIV-1)-infected children who had undetectable plasma HIV-1 RNA for more than 2 years while receiving highly active antiretroviral therapy. This research examines the in vitro effects of nucleoside reverse transcriptase inhibitors (NRTIs) on mitochondria of human skeletal muscle cells (HSMCs), including myoblasts and differentiated myotubes. mtDNA, mitochondrial RNA (mtRNA), and mRNA levels for nuclear mitochondrial regulatory factors were quantified in vitro using HSMCs, including myoblasts and differentiated myotubes, treated with NRTIs singly and in combination. After 5 days of treatment, mtDNA was significantly decreased in myoblasts and myotubes treated with ddI (P < 0.001 and P = 0.01, respectively) and ddI-containing regimens (P < 0.001 and P < 0.001, respectively) compared to levels in untreated cells. mtRNA (MTCYB) was also significantly decreased in the myoblasts and myotubes treated with ddI (P = 0.004) and ddI-containing regimens (P < 0.001). Regardless of the NRTI regimens examined, NRTI combinations significantly decreased mtRNA (MTCO3) in myoblasts and myotubes (P = 0.02 and P = 0.01, respectively). No significant differences were observed for nuclear mitochondrial regulatory factor mRNA in myoblasts or myotubes when treated with NRTIs (P > 0.07). ddI and ddI-containing regimens significantly decrease mtDNA and mtRNA in HSMCs, most notably in myoblasts. These findings may be of particular importance in developing countries, where ddI is widely used for first-line treatment of HIV-infected children.

scholarly journals Simplified in vitro engineering of neuromuscular junctions between rat embryonic motoneurons and immortalized human skeletal muscle cells

2019 ◽  
Vol Volume 12 ◽  
pp. 1-9 ◽  
Author(s):  
Jasdeep Saini ◽  
Alessandro Faroni ◽  
Marwah Abd Al Samid ◽  
Adam Reid ◽  
Adam Lightfoot ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Neuromuscular Junctions ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Human Skeletal Muscle Cells

scholarly journals Formoterol Stimulation In Vitro Influences Myogenic Regulatory Factors During Myogenesis In Human Skeletal Muscle Cells

2020 ◽  
Vol 52 (7S) ◽  
pp. 920-920
Author(s):  
Chase M. White ◽  
Gena D. Guerin ◽  
Emily L. Zumbro ◽  
Ryan A. Gordon ◽  
Dreanna M. McAdams ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Myogenic Regulatory Factors ◽  
Regulatory Factors ◽  
Human Skeletal Muscle Cells

Calf Blood Compound (CFC) and Homeopathic Drug Induce Differentiation of Primary Human Skeletal Muscle Cells

2019 ◽  
Vol 40 (12) ◽  
pp. 803-809 ◽  
Author(s):  
Eva K. Langendorf ◽  
Anja Klein ◽  
Pol M. Rommens ◽  
Philipp Drees ◽  
Ulrike Ritz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Cell Viability ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
In Vivo Studies ◽  
Human Skeletal Muscle Cells ◽  
Regulated Gene Expression

AbstractThe use of injections to treat structural muscle injuries is controversially discussed. In our controlled in vitro study, we investigated the biological impact of Actovegin and Traumeel alone and in combination on primary human skeletal muscle cells. Cells were characterized by immunofluorescence staining for myogenic factor 5 (Myf5) and MyoD, and cultured with or without Actovegin and / or Traumeel. The effects of these agents were assayed by cell viability and gene expression of the specific markers MyoD, Myf5, neural adhesion molecule (NCAM), and CD31. Myotube formation was determined by myosin staining. Neither Actovegin nor Traumeel showed toxic effects or influenced cell viability significantly. High volumes of Actovegin down-regulated gene expression of NCAM after 3 days but had no effect on MyoD, Myf5, and CD31 gene expression. High volumes of Traumeel inhibited MyoD gene expression after 3 days, whereas after 7 days MyoD expression was significantly up-regulated. The combination of both agents did not significantly influence cell viability or gene expression. This is the first study demonstrating that Actovegin and Traumeel potentially modulate human skeletal muscle cells. The relevance of these in vitro findings has to be highlighted in further in vivo studies.

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