scholarly journals Correction: Compositional Genome Contexts Affect Gene Expression Control in Sea Urchin Embryo

PLoS ONE ◽  
2009 ◽  
Vol 4 (1) ◽  
Author(s):  
Abdullah Al Mahmud ◽  
Gabriele Amore ◽  
Giorgio Bernardi
Keyword(s):  
Gene Expression ◽  
Sea Urchin ◽  
Expression Control ◽  
Sea Urchin Embryo ◽  
Gene Expression Control ◽  
Affect Gene Expression

scholarly journals Compositional Genome Contexts Affect Gene Expression Control in Sea Urchin Embryo

PLoS ONE ◽  
2008 ◽  
Vol 3 (12) ◽  
pp. e4025 ◽  
Author(s):  
Abdullah Al Mahmud ◽  
Gabriele Amore ◽  
Giorgio Bernardi
Keyword(s):  
Gene Expression ◽  
Sea Urchin ◽  
Expression Control ◽  
Sea Urchin Embryo ◽  
Gene Expression Control ◽  
Affect Gene Expression

scholarly journals High-performance chemical- and light-inducible recombinases in mammalian cells and mice

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Benjamin H. Weinberg ◽  
Jang Hwan Cho ◽  
Yash Agarwal ◽  
N. T. Hang Pham ◽  
Leidy D. Caraballo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
High Performance ◽  
Genome Engineering ◽  
Genetic Circuits ◽  
Control Of Gene Expression ◽  
Expression Control ◽  
Gene Expression Control ◽  
High Performance Systems ◽  
Spatiotemporal Control

Abstract Site-specific DNA recombinases are important genome engineering tools. Chemical- and light-inducible recombinases, in particular, enable spatiotemporal control of gene expression. However, inducible recombinases are scarce due to the challenge of engineering high performance systems, thus constraining the sophistication of genetic circuits and animal models that can be created. Here we present a library of >20 orthogonal inducible split recombinases that can be activated by small molecules, light and temperature in mammalian cells and mice. Furthermore, we engineer inducible split Cre systems with better performance than existing systems. Using our orthogonal inducible recombinases, we create a genetic switchboard that can independently regulate the expression of 3 different cytokines in the same cell, a tripartite inducible Flp, and a 4-input AND gate. We quantitatively characterize the inducible recombinases for benchmarking their performances, including computation of distinguishability of outputs. This library expands capabilities for multiplexed mammalian gene expression control.

scholarly journals Global quantification of mammalian gene expression control

Nature ◽  
2011 ◽  
Vol 473 (7347) ◽  
pp. 337-342 ◽  
Author(s):  
Björn Schwanhäusser ◽  
Dorothea Busse ◽  
Na Li ◽  
Gunnar Dittmar ◽  
Johannes Schuchhardt ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammalian Gene ◽  
Expression Control ◽  
Gene Expression Control ◽  
Mammalian Gene Expression

The ins and outs of gene expression control

2004 ◽  
Vol 22 (7) ◽  
pp. 824-826 ◽  
Author(s):  
Francine B Perler
Keyword(s):  
Gene Expression ◽  
Expression Control ◽  
Gene Expression Control

scholarly journals Synthetic gene regulatory networks in the opportunistic human pathogen Streptococcus pneumoniae

2019 ◽  
Author(s):  
Robin A. Sorg ◽  
Clement Gallay ◽  
Jan-Willem Veening
Keyword(s):  
Gene Expression ◽  
Streptococcus Pneumoniae ◽  
Regulatory Networks ◽  
Expression Patterns ◽  
Combinatorial Libraries ◽  
Regulatory Elements ◽  
Expression Control ◽  
Gene Expression Control

AbstractStreptococcus pneumoniae can cause disease in various human tissues and organs, including the ear, the brain, the blood and the lung, and thus in highly diverse and dynamic environments. It is challenging to study how pneumococci control virulence factor expression, because cues of natural environments and the presence of an immune system are difficult to simulate in vitro. Here, we apply synthetic biology methods to reverse-engineer gene expression control in S. pneumoniae. A selection platform is described that allows for straightforward identification of transcriptional regulatory elements out of combinatorial libraries. We present TetR- and LacI-regulated promoters that show expression ranges of four orders of magnitude. Based on these promoters, regulatory networks of higher complexity are assembled, such as logic AND and IMPLY gates. Finally, we demonstrate single-copy genome-integrated toggle switches that give rise to bimodal population distributions. The tools described here can be used to mimic complex expression patterns, such as the ones found for pneumococcal virulence factors, paving the way for in vivo investigations of the importance of gene expression control on the pathogenicity of S. pneumoniae.

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