scholarly journals Synaptotagmin-1 and -7 Are Redundantly Essential for Maintaining the Capacity of the Readily-Releasable Pool of Synaptic Vesicles

PLoS Biology ◽  
2015 ◽  
Vol 13 (10) ◽  
pp. e1002267 ◽  
Author(s):  
Taulant Bacaj ◽  
Dick Wu ◽  
Jacqueline Burré ◽  
Robert C. Malenka ◽  
Xinran Liu ◽  
...  
Keyword(s):  
Synaptic Vesicles ◽  
Releasable Pool ◽  
Readily Releasable Pool ◽  
Synaptotagmin 1

A Simple Depletion Model of the Readily Releasable Pool of Synaptic Vesicles Cannot Account for Paired-Pulse Depression

2007 ◽  
Vol 97 (1) ◽  
pp. 948-950 ◽  
Author(s):  
Jane M. Sullivan
Keyword(s):  
Synaptic Vesicles ◽  
Hippocampal Neurons ◽  
Synaptic Activity ◽  
Excitatory Synapses ◽  
Short Term ◽  
Paired Pulse ◽  
Short Term Plasticity ◽  
Releasable Pool ◽  
Readily Releasable Pool ◽  
Paired Pulse Depression

Paired-pulse depression (PPD) is a form of short-term plasticity that plays a central role in processing of synaptic activity and is manifest as a decrease in the size of the response to the second of two closely timed stimuli. Despite mounting evidence to the contrary, PPD is still commonly thought to reflect depletion of the pool of synaptic vesicles available for release in response to the second stimulus. Here it is shown that PPD cannot be accounted for by depletion at excitatory synapses made by hippocampal neurons because PPD is unaffected by changes in the fraction of the readily releasable pool (RRP) released by the first of a pair of pulses.

scholarly journals Synaptotagmin 1 clamps synaptic vesicle fusion in mammalian neurons independent of complexin

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Nicholas A. Courtney ◽  
Huan Bao ◽  
Joseph S. Briguglio ◽  
Edwin R. Chapman
Keyword(s):  
Synaptic Vesicle ◽  
Vesicle Fusion ◽  
Snare Proteins ◽  
Major Focus ◽  
C2 Domains ◽  
Releasable Pool ◽  
Readily Releasable Pool ◽  
Synaptotagmin 1 ◽  
Synaptic Vesicle Fusion ◽  
C2a Domain

Abstract Synaptic vesicle (SV) exocytosis is mediated by SNARE proteins. Reconstituted SNAREs are constitutively active, so a major focus has been to identify fusion clamps that regulate their activity in synapses: the primary candidates are synaptotagmin (syt) 1 and complexin I/II. Syt1 is a Ca2+ sensor for SV release that binds Ca2+ via tandem C2-domains, C2A and C2B. Here, we first determined whether these C2-domains execute distinct functions. Remarkably, the C2B domain profoundly clamped all forms of SV fusion, despite synchronizing residual evoked release and rescuing the readily-releasable pool. Release was strongly enhanced by an adjacent C2A domain, and by the concurrent binding of complexin to trans-SNARE complexes. Knockdown of complexin had no impact on C2B-mediated clamping of fusion. We postulate that the C2B domain of syt1, independent of complexin, is the molecular clamp that arrests SVs prior to Ca2+-triggered fusion.

scholarly journals The readily releasable pool of synaptic vesicles

2017 ◽  
Vol 43 ◽  
pp. 63-70 ◽  
Author(s):  
Pascal S Kaeser ◽  
Wade G Regehr
Keyword(s):  
Synaptic Vesicles ◽  
Releasable Pool ◽  
Readily Releasable Pool

scholarly journals β-Adrenergic Receptors/Epac Signaling Increases the Size of the Readily Releasable Pool of Synaptic Vesicles Required for Parallel Fiber LTP

2020 ◽  
Vol 40 (45) ◽  
pp. 8604-8617
Author(s):  
Ricardo Martín ◽  
Nuria García-Font ◽  
Alberto Samuel Suárez-Pinilla ◽  
David Bartolomé-Martín ◽  
José Javier Ferrero ◽  
...  
Keyword(s):  
Synaptic Vesicles ◽  
Adrenergic Receptors ◽  
Parallel Fiber ◽  
Releasable Pool ◽  
Readily Releasable Pool ◽  
Β Adrenergic Receptors ◽  
Epac Signaling

scholarly journals Endogenous Rho-Kinase Signaling Maintains Synaptic Strength by Stabilizing the Size of the Readily Releasable Pool of Synaptic Vesicles

2012 ◽  
Vol 32 (1) ◽  
pp. 68-84 ◽  
Author(s):  
D. Gonzalez-Forero ◽  
F. Montero ◽  
V. Garcia-Morales ◽  
G. Dominguez ◽  
L. Gomez-Perez ◽  
...  
Keyword(s):  
Synaptic Vesicles ◽  
Rho Kinase ◽  
Synaptic Strength ◽  
Kinase Signaling ◽  
Releasable Pool ◽  
Readily Releasable Pool

scholarly journals The Bruchpilot cytomatrix determines the size of the readily releasable pool of synaptic vesicles

2013 ◽  
Vol 202 (4) ◽  
pp. 667-683 ◽  
Author(s):  
Tanja Matkovic ◽  
Matthias Siebert ◽  
Elena Knoche ◽  
Harald Depner ◽  
Sara Mertel ◽  
...  
Keyword(s):  
Synaptic Vesicles ◽  
Circular Array ◽  
Membrane Domain ◽  
Releasable Pool ◽  
Readily Releasable Pool ◽  
Family Protein ◽  
Biochemical Identification ◽  
Macromolecular Architecture ◽  
Bar Formation ◽  
Ca2 Channels

Synaptic vesicles (SVs) fuse at a specialized membrane domain called the active zone (AZ), covered by a conserved cytomatrix. How exactly cytomatrix components intersect with SV release remains insufficiently understood. We showed previously that loss of the Drosophila melanogaster ELKS family protein Bruchpilot (BRP) eliminates the cytomatrix (T bar) and declusters Ca2+ channels. In this paper, we explored additional functions of the cytomatrix, starting with the biochemical identification of two BRP isoforms. Both isoforms alternated in a circular array and were important for proper T-bar formation. Basal transmission was decreased in isoform-specific mutants, which we attributed to a reduction in the size of the readily releasable pool (RRP) of SVs. We also found a corresponding reduction in the number of SVs docked close to the remaining cytomatrix. We propose that the macromolecular architecture created by the alternating pattern of the BRP isoforms determines the number of Ca2+ channel-coupled SV release slots available per AZ and thereby sets the size of the RRP.

scholarly journals Superpriming of synaptic vesicles after their recruitment to the readily releasable pool

2013 ◽  
Vol 110 (37) ◽  
pp. 15079-15084 ◽  
Author(s):  
J. S. Lee ◽  
W.-K. Ho ◽  
E. Neher ◽  
S.-H. Lee
Keyword(s):  
Synaptic Vesicles ◽  
Releasable Pool ◽  
Readily Releasable Pool

scholarly journals Lack of Synapsin I Reduces the Readily Releasable Pool of Synaptic Vesicles at Central Inhibitory Synapses

2007 ◽  
Vol 27 (49) ◽  
pp. 13520-13531 ◽  
Author(s):  
P. Baldelli ◽  
A. Fassio ◽  
F. Valtorta ◽  
F. Benfenati
Keyword(s):  
Synaptic Vesicles ◽  
Inhibitory Synapses ◽  
Synapsin I ◽  
Releasable Pool ◽  
Readily Releasable Pool

scholarly journals Long-Term Culture of Astrocytes Attenuates the Readily Releasable Pool of Synaptic Vesicles

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e48034 ◽  
Author(s):  
Hiroyuki Kawano ◽  
Shutaro Katsurabayashi ◽  
Yasuhiro Kakazu ◽  
Yuta Yamashita ◽  
Natsuko Kubo ◽  
...  
Keyword(s):  
Synaptic Vesicles ◽  
Long Term Culture ◽  
Releasable Pool ◽  
Readily Releasable Pool
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