Abstract
Background: Wilms’ Tumor Gene 1 (WT1) is a potential valuable parameter in prognosis of childhood acute lymphoblastic leukemia (ALL). However, studies on prevalence of WT1 and its correlation to clinical features and prognosis in pediatric patients were not well done. In this study we attempted to identify the correlation between WT1 and childhood ALL.Methods: The expression levels of WT1 in bone marrow cells of 188 children diagnosed with ALL from 2015 to 2018 were detected using real-time quantitative polymerase chain reaction (RQ-PCR). The relationship between expression levels of WT1 and patients’ characteristics, remission status (complete remission/relapse), fusion genes and prognosis of childhood ALL were analyzed and revealed. Results: 1. 147 (78.2%) cases had positive WT1 expression, and the average level was 1.76 (0.3, 6.03) %. 2. The CR and relapse rates of ALL children with positive WT1 were not significantly different from those of WT1 negative group, respectively (87.76% vs 82.93%, P=0.42 and 14.29% vs 17.1%, P=0.658). 3. The WT1 expression level in patients at CR was significantly lower than when at diagnosis (P<0.001) and the expression of WT1 increased obviously after induction therapy in 21 patients who relapsed (P=0.003) .4. The WT1 expression was related to lymphadenectasis (P=0.004) and immunophenotyping (P=0.009), but not to fusion genes (P=0.912). Conclusion: The WT1 in ALL children can be employed as an independent tool to evaluate the prognosis and curative effect of the disease.
The Wilms Tumor Gene, Wt1, Is Critical for Mouse Spermatogenesis via Regulation of Sertoli Cell Polarity and Is Associated with Non-Obstructive Azoospermia in Humans
