Wilms’ tumor gene WT1 17AA(-)/KTS(-) isoform induces morphological changes and promotes cell migration and invasion in vitro

Triple-negative breast cancer (TNBC) is a refractory type of breast cancer that does not yet have clinically effective drugs. The aim of this study is to investigate the synergistic effects and mechanisms of resveratrol combined with cisplatin on human breast cancer MDA-MB-231 (MDA231) cell viability, migration, and invasion in vivo and in vitro. In vitro, MTS assays showed that resveratrol combined with cisplatin inhibits cell viability as a concentration-dependent manner, and produced synergistic effects (CI < 1). Transwell assay showed that the combined treatment inhibits TGF-β1-induced cell migration and invasion. Immunofluorescence assays confirmed that resveratrol upregulated E-cadherin expression and downregulated vimentin expression. Western blot assay demonstrated that resveratrol combined with cisplatin significantly reduced the expression of fibronectin, vimentin, P-AKT, P-PI3K, P-JNK, P-ERK, Sma2, and Smad3 induced by TGF-β1 (p < 0.05), and increased the expression of E-cadherin (p < 0.05), respectively. In vivo, resveratrol enhanced tumor growth inhibition and reduced body weight loss and kidney function impairment by cisplatin in MDA231 xenografts, and significantly reduced the expressions of P-AKT, P-PI3K, Smad2, Smad3, P-JNK, P-ERK, and NF-κB in tumor tissues (p < 0.05). These results indicated that resveratrol combined with cisplatin inhibits the viability of breast cancer MDA231 cells synergistically, and inhibits MDA231 cells invasion and migration through Epithelial-mesenchymal transition (EMT) approach, and resveratrol enhanced anti-tumor effect and reduced side of cisplatin in MDA231 xenografts. The mechanism may be involved in the regulations of PI3K/AKT, JNK, ERK and NF-κB expressions.

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microRNA-18a Promotes Cell Migration and Invasion Through Inhibiting Dicer l Expression in Hepatocellular Carcinoma In Vitro△

Chinese Medical Sciences Journal ◽

10.24920/j1001-9242.2007.005 ◽

2017 ◽

Vol 32 (1) ◽

pp. 34-43 ◽

Cited By ~ 5

Author(s):

Xiufen Zhang ◽

Bo Yu ◽

Fuzheng Zhang ◽

Zijian Guo ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Cell Migration ◽

Migration And Invasion ◽

Cell Migration And Invasion

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Akebia trifoliata (Thunb.) Koidz Seed Extract inhibits human hepatocellular carcinoma cell migration and invasion in vitro

Journal of Ethnopharmacology ◽

10.1016/j.jep.2018.11.044 ◽

2019 ◽

Vol 234 ◽

pp. 204-215 ◽

Cited By ~ 4

Author(s):

Wen-Li Lu ◽

Tao Yang ◽

Qiu-Jia Song ◽

Zhao-Qin Fang ◽

Zhi-Qiang Pan ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Cell Migration ◽

Carcinoma Cell ◽

Seed Extract ◽

Human Hepatocellular Carcinoma ◽

Migration And Invasion ◽

Cell Migration And Invasion ◽

Human Hepatocellular Carcinoma Cell ◽

Hepatocellular Carcinoma Cell

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Vaccinium angustifolium (lowbush blueberry) leaf extract increases extravillous trophoblast cell migration and invasion in vitro

Phytotherapy Research ◽

10.1002/ptr.6021 ◽

2018 ◽

Vol 32 (4) ◽

pp. 705-714 ◽

Cited By ~ 3

Author(s):

Christina Ly ◽

Jonathan Ferrier ◽

Jeremiah Gaudet ◽

Julien Yockell-Lelièvre ◽

John Thor Arnason ◽

...

Keyword(s):

Cell Migration ◽

Leaf Extract ◽

Trophoblast Cell ◽

Extravillous Trophoblast ◽

Migration And Invasion ◽

Vaccinium Angustifolium ◽

Cell Migration And Invasion ◽

Lowbush Blueberry

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Sensitivity to imatinib therapy may be predicted by testing Wilms tumor gene expression and colony growth after a short in vitro incubation

Cancer ◽

10.1002/cncr.20457 ◽

2004 ◽

Vol 101 (5) ◽

pp. 979-988 ◽

Cited By ~ 26

Author(s):

Daniela Cilloni ◽

Francesca Messa ◽

Enrico Gottardi ◽

Milena Fava ◽

Francesca Arruga ◽

...

Keyword(s):

Gene Expression ◽

Wilms Tumor ◽

Colony Growth ◽

Imatinib Therapy ◽

Wilms Tumor Gene ◽

In Vitro Incubation ◽

Tumor Gene Expression ◽

Tumor Gene

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Acyl-CoA Synthetase 5 Promotes the Growth and Invasion of Colorectal Cancer Cells

Canadian Journal of Gastroenterology and Hepatology ◽

10.1155/2017/7615736 ◽

2017 ◽

Vol 2017 ◽

pp. 1-14 ◽

Cited By ~ 3

Author(s):

Shihua Ding ◽

Shaohui Tang ◽

Min Wang ◽

Donghai Wu ◽

Haijian Guo

Keyword(s):

Colorectal Cancer ◽

Cell Proliferation ◽

Cell Migration ◽

Cell Apoptosis ◽

Migration And Invasion ◽

Cell Migration And Invasion ◽

Sw620 Cells ◽

Role Of It

Background and Aims. Acyl-CoA synthetase 5 (ACS5) has been reported to be associated with the development of various cancers, but the role of it in colorectal cancer (CRC) is not well understood. The present study aimed to explore the potential role of ACS5 in the development and progression of CRC. Methods. ACS5 expression in CRC tissues and CRC cell lines was examined, and its clinical significance was analyzed. The role of ACS5 in cell proliferation, apoptosis, and invasion was examined in vitro. Results. We found that ACS5 expression was upregulated in CRC cells and CRC tissues and that high ACS5 expression was more frequent in CRC patients with excess muscular layer and with poor tumor differentiation. Furthermore, knockdown of ACS5 in HT29 and SW480 cells significantly dampened cell proliferation, induced cell apoptosis, and reduced cell migration and invasion. In contrast, the ectopic overexpression of ACS5 in LOVO and SW620 cells remarkably promoted cell proliferation, inhibited cell apoptosis, and enhanced cell migration and invasion. Enhanced cell growth and invasion ability mediated by the gain of ACS5 expression were associated with downregulation of caspase-3 and E-cadherin and upregulation of survivin and CD44. Conclusions. Our data demonstrate that ACS5 can promote the growth and invasion of CRC cells and provide a potential target for CRC gene therapy.

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Polydatin, a Glycoside of Resveratrol, Induces Apoptosis and Inhibits Metastasis Oral Squamous Cell Carcinoma Cells In Vitro

Pharmaceuticals ◽

10.3390/ph14090902 ◽

2021 ◽

Vol 14 (9) ◽

pp. 902

Author(s):

Tae-Hyun Bang ◽

Bong-Soo Park ◽

Hae-Mi Kang ◽

Jung-Han Kim ◽

In-Ryoung Kim

Keyword(s):

Cell Migration ◽

Side Effects ◽

Oral Cancer ◽

Cancer Treatment ◽

Migration And Invasion ◽

Related Factor ◽

Related Factors ◽

Cell Migration And Invasion ◽

Snail Proteins

Although various methods, such as surgery and chemotherapy, are applied to the treatment of OSCC, there are problems, such as functional and aesthetic limitations of the mouth and face, drug side effects, and lymph node metastasis. Many researchers are making efforts to develop new therapeutic agents from plant-derived substances to overcome the side effects that occur in oral cancer treatment. Polydatin is known as a natural precursor of resveratrol, and research on its efficacy is being actively conducted recently. Therefore, we investigated whether polydatin can induce apoptosis and whether it affects cell migration and invasion through the regulation of EMT-related factors in OSCC. Polydatin decreased the survival and proliferation rates of CAL27 and Ca9-22 cells, and induced the release of cytochrome c, a factor related to apoptosis, and fragmentation of procaspase-3 and PARP. Another form of cell death, autophagy, was observed in polydatin-treated cells. In addition, polydatin inhibits cell migration and invasion, and it has been shown to occur through increased expression of E-cadherin, an EMT related factor, and decreased expression of N-cadherin and Slug and Snail proteins and genes. These findings suggest that polydatin is a potential oral cancer treatment.

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