Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype

world. We have evaluated the combination of one-step RT-PCR and multiplex nested PCR assays for detectingdengue viruses from clinical samples. Twelve patients were screened for the dengue virus, using a pair of primersthat conserve for several Flavivirus. The results showed that in 12 suspect patients, 100% were positive for Flavivirusand there are some genotypic variation among them, that indicated by several RT-PCR products higher than 511 bp,the expected product for RT-PCR. Further assay was performed to clarify the presence and serotypes of dengue virususing multiplex nested PCR. Serotyping results indicated that 83,3% of samples can be confirmed for dengue virus.Among the dengue virus positive 16,7 % are dengue-2, 16.7 % are dengue-3, and the most common 50% are dengue-4,whereas dengue-1 were not found among the patients. The combination of RT-PCR and multiplex nested PCR assaycan be used for rapid analysis dengue samples in early phase which is potentially useful for clinical, epidemiologyand also evolutionary studies.Key words: Flavivirus, dengue virus, serotype, RT-PCR, multiplex nested PCR

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A one-step TaqMan real-time qRT-PCR assay for the specific detection and quantitation of the Spanish goat encephalitis virus (SGEV)

Journal of Virological Methods ◽

10.1016/j.jviromet.2018.02.017 ◽

2018 ◽

Vol 255 ◽

pp. 98-100

Author(s):

Ana del Cerro ◽

Ana Balseiro ◽

Rosa Casais ◽

Kevin P. Dalton ◽

Luis Salinas ◽

...

Keyword(s):

Real Time ◽

Encephalitis Virus ◽

Specific Detection ◽

Pcr Assay ◽

Qrt Pcr ◽

One Step ◽

Spanish Goat

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Spatio-temporal distribution analysis of circulating genotypes of dengue virus type 1 in western and southern states of India by a one-step real-time RT-PCR assay

Infection Genetics and Evolution ◽

10.1016/j.meegid.2019.103989 ◽

2019 ◽

Vol 75 ◽

pp. 103989 ◽

Cited By ~ 2

Author(s):

K. Alagarasu ◽

J.A. Patil ◽

M.B. Kakade ◽

A.M. More ◽

M. Bote ◽

...

Keyword(s):

Dengue Virus ◽

Real Time ◽

Temporal Distribution ◽

Distribution Analysis ◽

Rt Pcr ◽

Pcr Assay ◽

Southern States ◽

One Step ◽

Spatio Temporal

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Development and Validation of a One-Step Real-Time PCR Assay for Simultaneous Detection of Subtype H5, H7, and H9 Avian Influenza Viruses

Journal of Clinical Microbiology ◽

10.1128/jcm.02204-07 ◽

2008 ◽

Vol 46 (5) ◽

pp. 1769-1773 ◽

Cited By ~ 94

Author(s):

I. Monne ◽

S. Ormelli ◽

A. Salviato ◽

C. De Battisti ◽

F. Bettini ◽

...

Keyword(s):

Avian Influenza ◽

Real Time ◽

Real Time Pcr ◽

Simultaneous Detection ◽

Influenza Viruses ◽

Avian Influenza Viruses ◽

Pcr Assay ◽

One Step ◽

Development And Validation

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Development and validation of a Myxoma virus real-time polymerase chain reaction assay

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638711425946 ◽

2011 ◽

Vol 24 (1) ◽

pp. 135-137 ◽

Cited By ~ 6

Author(s):

Sarah Albini ◽

Brigitte Sigrist ◽

Regula Güttinger ◽

Claude Schelling ◽

Richard K. Hoop ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Real Time ◽

Rapid Diagnosis ◽

Myxoma Virus ◽

The Novel ◽

Specific Detection ◽

Chain Reaction ◽

Polymerase Chain ◽

Development And Validation ◽

Rabbit Tissues

To aid in the rapid diagnosis of myxomatosis in rabbits, a real-time polymerase chain reaction (PCR) for the specific detection of Myxoma virus is described. Primers and probe were designed to amplify a 147-bp fragment within the Serp2 gene. The assay was able to detect 23 copies of a synthesized oligo indicating a reliable sensitivity. In addition, the real-time PCR did not detect the Rabbit fibroma virus used in myxomatosis vaccines. The novel PCR was shown to be able to detect Myxoma virus in fresh and paraffin-embedded rabbit tissues originating from myxomatosis cases from various regions in Switzerland.

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