Development and Validation of Real-Time RT-LAMP Assays for the Specific Detection of Zika Virus

2020 ◽  
pp. 147-164
Author(s):  
Benjamin Lopez-Jimena ◽  
Mohammed Bakheit ◽  
Michaël Bekaert ◽  
Graham Harold ◽  
Sieghard Frischmann ◽  
...  
Keyword(s):  
Real Time ◽  
Zika Virus ◽  
Specific Detection ◽  
Development And Validation

Development and validation of a rapid real-time PCR based method for the specific detection of Salmonella on fresh meat

Meat Science ◽  
2009 ◽  
Vol 83 (3) ◽  
pp. 555-562 ◽  
Author(s):  
Sheila McGuinness ◽  
Evonne McCabe ◽  
Edel O’Regan ◽  
Anthony Dolan ◽  
Geraldine Duffy ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Specific Detection ◽  
Fresh Meat ◽  
Development And Validation

scholarly journals Development and validation of a Myxoma virus real-time polymerase chain reaction assay

2011 ◽  
Vol 24 (1) ◽  
pp. 135-137 ◽  
Author(s):  
Sarah Albini ◽  
Brigitte Sigrist ◽  
Regula Güttinger ◽  
Claude Schelling ◽  
Richard K. Hoop ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Rapid Diagnosis ◽  
Myxoma Virus ◽  
The Novel ◽  
Specific Detection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Development And Validation ◽  
Rabbit Tissues

To aid in the rapid diagnosis of myxomatosis in rabbits, a real-time polymerase chain reaction (PCR) for the specific detection of Myxoma virus is described. Primers and probe were designed to amplify a 147-bp fragment within the Serp2 gene. The assay was able to detect 23 copies of a synthesized oligo indicating a reliable sensitivity. In addition, the real-time PCR did not detect the Rabbit fibroma virus used in myxomatosis vaccines. The novel PCR was shown to be able to detect Myxoma virus in fresh and paraffin-embedded rabbit tissues originating from myxomatosis cases from various regions in Switzerland.

scholarly journals Development and Validation of a TaqMan Real-Time PCR Assay for the Specific Detection and Quantification of Fusarium fujikuroi in Rice Plants and Seeds

2017 ◽  
Vol 107 (7) ◽  
pp. 885-892 ◽  
Author(s):  
Greice Amaral Carneiro ◽  
Slavica Matić ◽  
Giuseppe Ortu ◽  
Angelo Garibaldi ◽  
Davide Spadaro ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Fusarium Species ◽  
Fusarium Fujikuroi ◽  
Specific Detection ◽  
Pcr Assay ◽  
Bakanae Disease ◽  
Repeatability And Reproducibility ◽  
Development And Validation ◽  
Early Rice

Bakanae disease, which is caused by the seedborne pathogen Fusarium fujikuroi, is found throughout the world on rice. A TaqMan real-time PCR has been developed on the TEF 1-α gene to detect F. fujikuroi in different rice tissues. Three primer/probe sets were tested. The selected set produced an amplicon of 84 bp and was specific for F. fujikuroi with respect to eight Fusarium species of rice and six other rice common pathogens. The assay was validated for specificity, selectivity, sensitivity, repeatability, and reproducibility. The detection limit was set at 27.5 fg of DNA, which is approximately equivalent to one haploid genome of F. fujikuroi. The developed TaqMan real-time assay was able to efficiently detect and quantify F. fujikuroi from rice culms, leaves, roots, and seeds. At 1 week post-germination (wpg), the pathogen was more diffused in the green tissues, while at 3 wpg it was uniformly spread also in the roots. The highest concentration of F. fujikuroi was measured in the M6 cultivar, which showed around 1,450 fungal cells/g. The assay was sufficiently sensitive to detect a few genomic equivalents in the rice seeds, corresponding to 9.89 F. fujikuroi cells/g. The assay permitted bakanae disease to be detected in asymptomatic tissues at the early rice development stages.

scholarly journals Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype

2018 ◽  
Vol 12 (5) ◽  
pp. e0006381 ◽  
Author(s):  
Benjamin Lopez-Jimena ◽  
Michaël Bekaert ◽  
Mohammed Bakheit ◽  
Sieghard Frischmann ◽  
Pranav Patel ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Specific Detection ◽  
Virus Serotype ◽  
One Step ◽  
Development And Validation

Chromo-fluorogenic sensors for chemical warfare agents in real-time analysis: journey towards accurate detection and differentiation

2021 ◽  
Vol 57 (28) ◽  
pp. 3430-3444
Author(s):  
Vinod Kumar
Keyword(s):  
Real Time ◽  
Chemical Warfare Agents ◽  
Chemical Warfare ◽  
Chemical Probes ◽  
Time Analysis ◽  
Specific Detection ◽  
Success Stories ◽  
Real Time Analysis ◽  
Warfare Agents ◽  
Unique Chemical

This article describes our journey and success stories in the development of chemical warfare detection, detailing the range of unique chemical probes and methods explored to achieve the specific detection of individual agents in realistic environments.

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