Combined sterile insect technique and incompatible insect technique: The first proof-of-concept to suppress Aedes aegypti vector populations in semi-rural settings in Thailand

AbstractThe mosquito Aedes aegypti is the principal vector for arboviruses including dengue/yellow fever, chikungunya, and Zika virus, infecting hundreds of millions of people annually. Unfortunately, traditional control methodologies are insufficient, so innovative control methods are needed. To complement existing measures, here we develop a molecular genetic control system termed precision-guided sterile insect technique (pgSIT) in Aedes aegypti. PgSIT uses a simple CRISPR-based approach to generate flightless females and sterile males that are deployable at any life stage. Supported by mathematical models, we empirically demonstrate that released pgSIT males can compete, suppress, and even eliminate mosquito populations. This platform technology could be used in the field, and adapted to many vectors, for controlling wild populations to curtail disease in a safe, confinable, and reversible manner.

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Optimal Control of Aedes Aegypti Mosquitoes by Sterile Insect Technique, Insecticide and Larvicide

10.20906/cps/cilamce2015-0072 ◽

2015 ◽

Cited By ~ 1

Author(s):

Roberto Carlos Antunes Thomé ◽

Claudia Mazza Dias ◽

Edilson Fernandes Arruda ◽

Dayse Haime Pastore ◽

Hyun Mo Yang

Keyword(s):

Optimal Control ◽

Aedes Aegypti ◽

Sterile Insect Technique

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ASSESSING THE SUITABILITY OF STERILE INSECT TECHNIQUE APPLIED TOAEDES AEGYPTI

Journal of Biological System ◽

10.1142/s0218339008002691 ◽

2008 ◽

Vol 16 (04) ◽

pp. 565-577 ◽

Cited By ~ 19

Author(s):

CLAUDIA PIO FERREIRA ◽

HYUN MO YANG ◽

LOURDES ESTEVA

Keyword(s):

Biological Control ◽

Cellular Automata ◽

Aedes Aegypti ◽

Control Strategy ◽

Sterile Insect Technique ◽

Alternative Control ◽

Heterogeneous Distribution ◽

Insecticide Application ◽

Mosquito Behavior

The efficacy of biological control of Aedes aegypti mosquitoes using Sterile Insect Technique (SIT) is analyzed. This approach has shown to be very efficient on agricultural plagues and has become an alternative control strategy to the usual technique of insecticide application, which promotes resistance against chemical controls and is harmful to other species that live in the same mosquito habitat. By using a discrete cellular automata approach we have shown that in the case of Aedes aegypti, the spatially heterogeneous distribution of oviposition containers and the mosquito behavior, especially with respect to mating, make the application of STI difficult or impracticable.

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Efficiency of Two Larval Diets for Mass Rearing of Aedes aegypti in Support of Sterile Insect Technique (SIT)

Trends in Vector Research and Parasitology ◽

10.7243/2054-9881-8-1 ◽

2021 ◽

Vol 8 (1) ◽

pp. 1

Author(s):

Nahida Sultana ◽

Mahfuza Khan ◽

Md. Rezaul Karim ◽

Nazma Khatun

Keyword(s):

Aedes Aegypti ◽

Sterile Insect Technique ◽

Mass Rearing

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Wolbachia-mediated sterility suppresses Aedes aegypti populations in the urban tropics

10.1101/2021.06.16.21257922 ◽

2021 ◽

Author(s):

◽

Lee Ching Ng

Keyword(s):

Aedes Aegypti ◽

Vertical Distribution ◽

High Rise ◽

Promising Tool ◽

Incompatible Insect Technique ◽

Tropical Cities ◽

Dengue Control ◽

Housing Estates

Incompatible insect technique (IIT) via releases of male Wolbachia-infected mosquitoes is a promising tool for dengue control. In a three-year trial in Singaporean high-rise housing estates, we demonstrated that Wolbachia-based IIT dramatically reduces both wildtype Aedes aegypti populations [reductions of 92.7% (95% CI: 84.7%-95.8%) and 98.3% (97.7%-99.8%)] and dengue incidence [reductions of 71% (43%-87%) to 88% (57%-99%)] in the targeted areas. The study highlights the need to ensure adequate vertical distribution of released males in high-rise buildings, address immigration of wildtype females from neighboring areas, and prevent and mitigate stable establishment of Wolbachia in field mosquito populations. Our results demonstrate the potential of Wolbachia-based IIT (supplemented with irradiation, in Singapore's context) for strengthening dengue control in tropical cities, where dengue burden is the greatest.

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Temperature-Inducible Precision Guided Sterile Insect Technique

10.1101/2021.06.14.448312 ◽

2021 ◽

Author(s):

Nikolay P Kandul ◽

Junru Liu ◽

Omar Akbari

Keyword(s):

Drosophila Melanogaster ◽

Gold Standard ◽

Sterile Insect Technique ◽

Population Control ◽

Insect Population ◽

Proof Of Concept ◽

Control Programs ◽

True Breeding ◽

Genetic Cross ◽

Automate Production

Releases of sterile males are the gold standard for many insect population control programs, and precise sex sorting to remove females prior to male releases is essential to the success of these operations. To advance traditional methods for scaling the generation of sterile males, we previously described a CRISPR-mediated precision-guided sterile insect technique (pgSIT), in which Cas9 and gRNA strains are genetically crossed to generate sterile males for release. While effective at generating F1 sterile males, pgSIT requires a genetic cross between the two parental strains which requires maintenance and sexing of two strains in a factory. Therefore, to further advance pgSIT by removing this crossing step, here we describe a next-generation Temperature-Inducible pgSIT (TI-pgSIT) technology and demonstrate its proof-of-concept in Drosophila melanogaster. Importantly, we were able to develop a true-breeding strain for TI-pgSIT that eliminates the requirement for sex sorting, a feature that may help further automate production at scale.

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Dampak Iradiasi Sinar Gamma pada Produktivitas Aedes aegypti Jantan

Jurnal Vektor Penyakit ◽

10.22435/vektorp.v13i1.962 ◽

2019 ◽

Vol 13 (1) ◽

pp. 1-6

Author(s):

MIRNA WATI DEWI ◽

Susi Soviana ◽

Umi Cahyaningsih ◽

Ali Rahayu

Keyword(s):

Aedes Aegypti ◽

Dengue Fever ◽

Sterile Insect Technique ◽

Second Generation ◽

Gamma Ray ◽

Vector Borne Disease ◽

Egg Hatchability ◽

Negative Effect ◽

Gamma Ray Irradiation ◽

Vector Borne

Abstract Dengue fever is a vector-borne disease with Aedes aegypti as the main vector. Vector controls currently depended on insecticide. Considering the negative effect of insecticide, Sterile Insect Technique (SIT) was developed. This study was conducted to evaluate the effect of gamma-ray irradiation on the productivity of male Ae. aegypti. Male pupae age less than 15 hours were irradiated with 60 Gy and 70 Gy gamma-ray. When the pupae became adult, the sterile males mated with the same age females Aedes aegypti. Observation on fecundity, hatchability, and age was carried out until the second generation. Gamma-ray irradiation with the dose of 60 Gy and 70 Gy showed different effects on fecundity, egg hatchability, the emergence of the adult, and age of Aedes aegypti compared to control. Abstrak Demam berdarah merupakan penyakit tular vektor yang sampai saat ini masih menjadi masalah kesehatan secara global. Vektor utama yang berperan pada penyebaran penyakit DBD yaitu nyamuk Aedes aegytpi. Pengendalian vektor saat ini sangat bergantung pada penggunaan insektisida. Dampak negatif penggunaan insektisida menyebabkan pengembangan pengendalian vektor yang lain diantaranya yaitu Teknik Serangga Mandul (TSM). Penelitian ini dilakukan untuk menganalisis produktivitas Ae. aegypti jantan iradiasi hingga generasi kedua. Pupa jantan umur <15 jam diradiasi sinargamma dosis 60 Gy dan 70 Gy. Setelah menjadi nyamuk jantan dewasa segeradikawinkan dengan nyamuk betina tidak iradiasi dengan umur yang sama. Pengamatan dilakukan terutama terhadap fekunditas, daya tetas, kemunculan nyamuk, dan umurnyamuk hingga generasi kedua. Iradiasi sinar gamma dosis 60 Gy dan 70 Gymenghasilkan dampak yang berbeda terhadap fekunditas, daya tetas telur, kemunculan nyamuk dan umur nyamuk bila dibandingkan dengan kontrol.

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