Vector competence of Aedes aegypti from New Caledonia for the four recent circulating dengue virus serotypes

INTRODUCTION: To detect dengue virus, eggs of Aedes sp were collected in the city of Belo Horizonte, Brazil, in 2007. METHODS: Egg samples were subsequently hatched and the larvae were tested for the presence of dengue virus RNA by RT-PCR. RESULTS: Among the Aedes aegypti larvae samples, 163 (37.4%) out of 435 were positive, including 32 (10.9%) of 293 individual larvae samples concomitantly positive for two serotypes. CONCLUSIONS: Virological surveillance detecting coinfected vectors in the field could represent an important strategy for understanding the numerous factors involved in the transmission and clinical presentation of dengue.

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Comparative analysis of midgut bacterial communities of Aedes aegypti mosquito strains varying in vector competence to dengue virus

Parasitology Research ◽

10.1007/s00436-013-3428-x ◽

2013 ◽

Vol 112 (7) ◽

pp. 2627-2637 ◽

Cited By ~ 28

Author(s):

Shakti S. Charan ◽

Kiran D. Pawar ◽

David W. Severson ◽

Milind S. Patole ◽

Yogesh S. Shouche

Keyword(s):

Comparative Analysis ◽

Aedes Aegypti ◽

Dengue Virus ◽

Bacterial Communities ◽

Vector Competence

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Reciprocal Tripartite Interactions between the Aedes aegypti Midgut Microbiota, Innate Immune System and Dengue Virus Influences Vector Competence

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0001561 ◽

2012 ◽

Vol 6 (3) ◽

pp. e1561 ◽

Cited By ~ 178

Author(s):

Jose Luis Ramirez ◽

Jayme Souza-Neto ◽

Rolando Torres Cosme ◽

Jose Rovira ◽

Alma Ortiz ◽

...

Keyword(s):

Immune System ◽

Aedes Aegypti ◽

Dengue Virus ◽

Innate Immune System ◽

Vector Competence ◽

Innate Immune ◽

Tripartite Interactions

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Assessment of fitness and vector competence of a New Caledonia wMel Aedes aegypti strain before field-release

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0009752 ◽

2021 ◽

Vol 15 (9) ◽

pp. e0009752

Author(s):

Nicolas Pocquet ◽

Olivia O’Connor ◽

Heather A. Flores ◽

Jordan Tutagata ◽

Morgane Pol ◽

...

Keyword(s):

Aedes Aegypti ◽

Cytoplasmic Incompatibility ◽

New Caledonia ◽

Vector Competence ◽

Oral Feeding ◽

Maternal Transmission ◽

Strong Inhibitor ◽

Feeding Experiments ◽

Dengue Outbreaks ◽

Mosquito Saliva

Background Biological control programs involving Wolbachia-infected Aedes aegypti are currently deployed in different epidemiological settings. New Caledonia (NC) is an ideal location for the implementation and evaluation of such a strategy as the only proven vector for dengue virus (DENV) is Ae. aegypti and dengue outbreaks frequency and severity are increasing. We report the generation of a NC Wolbachia-infected Ae. aegypti strain and the results of experiments to assess the vector competence and fitness of this strain for future implementation as a disease control strategy in Noumea, NC. Methods/principal findings The NC Wolbachia strain (NC-wMel) was obtained by backcrossing Australian AUS-wMel females with New Caledonian Wild-Type (NC-WT) males. Blocking of DENV, chikungunya (CHIKV), and Zika (ZIKV) viruses were evaluated via mosquito oral feeding experiments and intrathoracic DENV challenge. Significant reduction in infection rates were observed for NC-wMel Ae. aegypti compared to WT Ae. aegypti. No transmission was observed for NC-wMel Ae. aegypti. Maternal transmission, cytoplasmic incompatibility, fertility, fecundity, wing length, and insecticide resistance were also assessed in laboratory experiments. Ae. aegypti NC-wMel showed complete cytoplasmic incompatibility and a strong maternal transmission. Ae. aegypti NC-wMel fitness seemed to be reduced compared to NC-WT Ae. aegypti and AUS-wMel Ae. aegypti regarding fertility and fecundity. However further experiments are required to assess it accurately. Conclusions/significance Our results demonstrated that the NC-wMel Ae. aegypti strain is a strong inhibitor of DENV, CHIKV, and ZIKV infection and prevents transmission of infectious viral particles in mosquito saliva. Furthermore, our NC-wMel Ae. aegypti strain induces reproductive cytoplasmic incompatibility with minimal apparent fitness costs and high maternal transmission, supporting field-releases in Noumea, NC.

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Feasibility of feeding Aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0007116 ◽

2019 ◽

Vol 13 (2) ◽

pp. e0007116 ◽

Cited By ~ 2

Author(s):

Kanya C. Long ◽

Juan Sulca ◽

Isabel Bazan ◽

Helvio Astete ◽

Hugo L. Jaba ◽

...

Keyword(s):

Aedes Aegypti ◽

Dengue Virus ◽

Vector Competence ◽

Human Volunteers

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Sialic Acid Expression in the MosquitoAedes aegyptiand Its Possible Role in Dengue Virus-Vector Interactions

BioMed Research International ◽

10.1155/2015/504187 ◽

2015 ◽

Vol 2015 ◽

pp. 1-16 ◽

Cited By ~ 11

Author(s):

Jorge Cime-Castillo ◽

Philippe Delannoy ◽

Guillermo Mendoza-Hernández ◽

Verónica Monroy-Martínez ◽

Anne Harduin-Lepers ◽

...

Keyword(s):

Sialic Acid ◽

Aedes Aegypti ◽

Dengue Virus ◽

Mammalian Cells ◽

Vector Competence ◽

Denv Infection ◽

Viral Disease ◽

Salivary Gland Extract ◽

Denv Serotypes ◽

Mosquito Saliva

Dengue fever (DF) is the most prevalent arthropod-borne viral disease which affects humans. DF is caused by the four dengue virus (DENV) serotypes, which are transmitted to the host by the mosquitoAedes aegyptithat has key roles in DENV infection, replication, and viral transmission (vector competence). Mosquito saliva also plays an important role during DENV transmission. In this study, we detected the presence of sialic acid (Sia) inAedes aegyptitissues, which may have an important role during DENV-vector competence. We also identified genome sequences encoding enzymes involved in Sia pathways. The cDNA forAedes aegyptiCMP-Sia synthase (CSAS) was amplified, cloned, and functionally evaluated via the complementation of LEC29.Lec32 CSAS-deficient CHO cells.AedesCSAS-transfected LEC29.Lec32 cells were able to express Sia moieties on the cell surface. Sequences related toα-2,6-sialyltransferase were detected in theAedes aegyptigenome. Likewise, we identified Sia-α-2,6-DENV interactions in different mosquito tissues. In addition, we evaluated the possible role of sialylated molecules in a salivary gland extract during DENV internalization in mammalian cells. The knowledge of early DENV-host interactions could facilitate a better understanding of viral tropism and pathogenesis to allow the development of new strategies for controlling DENV transmission.

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