scholarly journals A Recombinant Avian Infectious Bronchitis Virus Expressing a Heterologous Spike Gene Belonging to the 4/91 Serotype

PLoS ONE ◽  
2011 ◽  
Vol 6 (8) ◽  
pp. e24352 ◽  
Author(s):  
Maria Armesto ◽  
Sharon Evans ◽  
David Cavanagh ◽  
Abu-Bakr Abu-Median ◽  
Sarah Keep ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Avian Infectious Bronchitis Virus ◽  
Spike Gene ◽  
Infectious Bronchitis

scholarly journals Recombinant Avian Infectious Bronchitis Virus Expressing a Heterologous Spike Gene Demonstrates that the Spike Protein Is a Determinant of Cell Tropism

2003 ◽  
Vol 77 (16) ◽  
pp. 9084-9089 ◽  
Author(s):  
Rosa Casais ◽  
Brian Dove ◽  
David Cavanagh ◽  
Paul Britton
Keyword(s):  
Infectious Bronchitis Virus ◽  
Reverse Genetics ◽  
Cell Types ◽  
Spike Protein ◽  
Cell Tropism ◽  
Avian Infectious Bronchitis Virus ◽  
Spike Glycoprotein ◽  
Spike Gene ◽  
Infectious Bronchitis ◽  
Different Cell Types

ABSTRACT A recombinant infectious bronchitis virus (IBV), BeauR-M41(S), was generated using our reverse genetics system (R. Casais, V. Thiel, S. G. Siddell, D. Cavanagh, and P. Britton, J. Virol. 75:12359-12369, 2001), in which the ectodomain region of the spike gene from IBV M41-CK replaced the corresponding region of the IBV Beaudette genome. BeauR-M41(S) acquired the same cell tropism phenotype as IBV M41-CK in four different cell types, demonstrating that the IBV spike glycoprotein is a determinant of cell tropism.

Comparative Evaluation of In Vitro and In Vivo Assays for the Detection of Avian Infectious Bronchitis Virus as a Contaminant of Live Poultry Vaccines

1998 ◽  
Vol 26 (5) ◽  
pp. 629-634
Author(s):  
Emiliana Falcone ◽  
Edoardo Vignolo ◽  
Livia Di Trani ◽  
Simona Puzelli ◽  
Maria Tollis
Keyword(s):  
Infectious Bronchitis Virus ◽  
Serological Response ◽  
Avian Infectious Bronchitis Virus ◽  
Animal Testing ◽  
Rt Pcr ◽  
Pcr Assay ◽  
In Vivo Test ◽  
Infectious Bronchitis

A reverse transcriptase polymerase chain reaction (RT-PCR) assay specific for identifying avian infectious bronchitis virus (IBV) in poultry vaccines, and the serological response to IBV induced by the inoculation of chicks with a Newcastle disease vaccine spiked with the Massachusetts strain of IBV, were compared for their ability to detect IBV as a contaminant of avian vaccines. The sensitivity of the IBV-RT-PCR assay provided results which were at least equivalent to the biological effect produced by the inoculation of chicks, allowing this assay to be considered a valid alternative to animal testing in the quality control of avian immunologicals. This procedure can easily be adapted to detect a number of contaminants for which the in vivo test still represents the only available method of detection.

Avian infectious bronchitis virus: isolation of an apparently new variant in Italy

2000 ◽  
Vol 146 (7) ◽  
pp. 191-193 ◽  
Author(s):  
A. Zanella ◽  
R. Coaro ◽  
R. Marchi ◽  
G. Fabris ◽  
A. Lavazza
Keyword(s):  
Infectious Bronchitis Virus ◽  
Virus Isolation ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
New Variant

scholarly journals Research Note: Antibodies to Avian Infectious Bronchitis Virus in Pakistani Chickens

1987 ◽  
Vol 66 (4) ◽  
pp. 765-767 ◽  
Author(s):  
MOHAMMAD A. MUNEER ◽  
JOHN A. NEWMAN ◽  
SAGAR M. GOYAL ◽  
M. AJMAL
Keyword(s):  
Infectious Bronchitis Virus ◽  
Research Note ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis
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