scholarly journals Selection of Diethylstilbestrol-Specific Single-Chain Antibodies from a Non-Immunized Mouse Ribosome Display Library

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33186 ◽  
Author(s):  
Yanan Sun ◽  
Baoan Ning ◽  
Ming Liu ◽  
Xianjun Gao ◽  
Xianjun Fan ◽  
...  
Keyword(s):  
Single Chain ◽  
Ribosome Display ◽  
Single Chain Antibodies ◽  
Selection Of

scholarly journals Generation and Selection of a Panel of Pan-Filovirus Single-Chain Antibodies using Cell-Free Ribosome Display

2018 ◽  
Author(s):  
Adinarayana Kunamneni ◽  
Elizabeth C. Clarke ◽  
Chunyan Ye ◽  
Steven B. Bradfute ◽  
Ravi Durvasula
Keyword(s):  
Hemorrhagic Fever ◽  
Cross Reactivity ◽  
Free Ribosome ◽  
Rapid Diagnostics ◽  
Single Chain ◽  
Ribosome Display ◽  
Single Chain Antibodies ◽  
Single Chain Fv ◽  
New Generation ◽  
Selection Of

AbstractFiloviruses, which include ebolaviruses and marburgvirus, can cause outbreaks of highly lethal hemorrhagic fever. This disease causes significant morbidity and mortality in humans and non-human primates, with human fatality rates reaching 90% during some outbreaks. Currently, there are a lack of licensed vaccines or antivirals for these viruses. Since early symptoms of filovirus infection mimic more common diseases, there is a strong unmet public health and biodefense need for broad-spectrum filovirus rapid diagnostics. We have generated a panel of mouse single-chain Fv-antibodies (scFvs) to filovirus glycoproteins (GPs) using cell-free ribosome display and determined their cross-reactivity profiles to all known filovirus species. Two scFvs (4-2 and 22-1) were able to detect all known Ebolavirus and Marburgvirus species. This is the first report on ribosome display scFvs that can detect a broad set of filovirus GPs, which demonstrates their potential use in the development of a new generation of rapid diagnostic immunoassays.

Selection of bisphenol A – single-chain antibodies from a non-immunized mouse library by ribosome display

2015 ◽  
Vol 488 ◽  
pp. 59-64 ◽  
Author(s):  
Li Zhao ◽  
Baoan Ning ◽  
Jialei Bai ◽  
Xiang Chen ◽  
Yuan Peng ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Single Chain ◽  
Ribosome Display ◽  
Single Chain Antibodies ◽  
Selection Of

scholarly journals Generation and Selection of a Panel of Pan-Filovirus Single-Chain Antibodies using Cell-Free Ribosome Display

2019 ◽  
Vol 101 (1) ◽  
pp. 198-206 ◽  
Author(s):  
Adinarayana Kunamneni ◽  
Elizabeth C. Clarke ◽  
Chunyan Ye ◽  
Steven B. Bradfute ◽  
Ravi Durvasula
Keyword(s):  
Free Ribosome ◽  
Single Chain ◽  
Ribosome Display ◽  
Single Chain Antibodies ◽  
Selection Of

Selection of Single Chain Variable Fragments Specific for the Human-Inducible Costimulator Using Ribosome Display

2012 ◽  
Vol 168 (5) ◽  
pp. 967-979 ◽  
Author(s):  
Yangbin Pan ◽  
Weiping Mao ◽  
Xuanxuan Liu ◽  
Chong Xu ◽  
Zhijuan He ◽  
...  
Keyword(s):  
Single Chain ◽  
Ribosome Display ◽  
Inducible Costimulator ◽  
Single Chain Variable Fragments ◽  
Selection Of

scholarly journals Ribosome Display of Combinatorial Antibody Libraries Derived from Mice Immunized with Heat-Killed Xylella fastidiosa and the Selection of MopB-Specific Single-Chain Antibodies

2012 ◽  
Vol 78 (8) ◽  
pp. 2638-2647 ◽  
Author(s):  
Armaghan Azizi ◽  
Arinder Arora ◽  
Anatoliy Markiv ◽  
David J. Lampe ◽  
Thomas A. Miller ◽  
...  
Keyword(s):  
Xylella Fastidiosa ◽  
Pierce's Disease ◽  
Single Chain ◽  
Ribosome Display ◽  
Content Type ◽  
Single Chain Antibodies ◽  
Pierce’S Disease ◽  
Antibody Libraries ◽  
Potential Use

ABSTRACTPierce's disease is a devastating lethal disease ofVitus viniferagrapevines caused by the bacteriumXylella fastidiosa. There is no cure for Pierce's disease, and control is achieved predominantly by suppressing transmission of the glassy-winged sharpshooter insect vector. We present a simple robust approach for the generation of panels of recombinant single-chain antibodies against the surface-exposed elements ofX. fastidiosathat may have potential use in diagnosis and/or disease transmission blocking studies.In vitrocombinatorial antibody ribosome display libraries were assembled from immunoglobulin transcripts rescued from the spleens of mice immunized with heat-killedX. fastidiosa. The libraries were used in a single round of selection against an outer membrane protein, MopB, resulting in the isolation of a panel of recombinant antibodies. The potential use of selected anti-MopB antibodies was demonstrated by the successful application of the 4XfMopB3 antibody in an enzyme-linked immunosorbent assay (ELISA), a Western blot assay, and an immunofluorescence assay (IFA). These immortalizedin vitrorecombinant single-chain antibody libraries generated against heat-killedX. fastidiosaare a resource for the Pierce's disease research community that may be readily accessed for the isolation of antibodies against a plethora ofX. fastidiosasurface-exposed antigenic molecules.

scholarly journals Selection of Single-Chain Antibodies against the VP8* Subunit of Rotavirus VP4 Outer Capsid Protein and Their Expression in Lactobacillus casei

2004 ◽  
Vol 70 (11) ◽  
pp. 6936-6939 ◽  
Author(s):  
Vicente Monedero ◽  
Jes�s Rodr�guez-D�az ◽  
Rosa Viana ◽  
Javier Buesa ◽  
Gaspar P�rez-Mart�nez
Keyword(s):  
Lactobacillus Casei ◽  
Probiotic Bacteria ◽  
Extracellular Expression ◽  
Outer Capsid Protein ◽  
Single Chain ◽  
Single Chain Antibodies ◽  
Outer Capsid ◽  
Selection Of

ABSTRACT Single-chain antibodies (scFv) recognizing the VP8* fraction of rotavirus outer capsid and blocking rotavirus infection in vitro were isolated by phage display. Vectors for the extracellular expression in Lactobacillus casei of one of the scFv were constructed. L. casei was able to secrete active scFv to the growth medium, showing the potential of probiotic bacteria to be engineered to express molecules suitable for in vivo antirotavirus therapies.

scholarly journals Ribosome display and selection of single-chain variable fragments effectively inhibit growth and progression of microspheres in vitro and in vivo

2018 ◽  
Vol 109 (5) ◽  
pp. 1503-1512
Author(s):  
Shangke Huang ◽  
Lu Feng ◽  
Gaili An ◽  
Xiaojin Zhang ◽  
Zixuan Zhao ◽  
...  
Keyword(s):  
Single Chain ◽  
Ribosome Display ◽  
Single Chain Variable Fragments ◽  
Selection Of

In vivo selection of single-chain antibodies using a yeast two-hybrid system

2000 ◽  
Vol 238 (1-2) ◽  
pp. 161-172 ◽  
Author(s):  
Antje Pörtner-Taliana ◽  
Marijane Russell ◽  
Karen J Froning ◽  
Paul R Budworth ◽  
John D Comiskey ◽  
...  
Keyword(s):  
Hybrid System ◽  
Single Chain ◽  
Yeast Two Hybrid ◽  
Single Chain Antibodies ◽  
In Vivo Selection ◽  
Yeast Two Hybrid System ◽  
Two Hybrid ◽  
Selection Of
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