Genetic Diversity of the ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus Isolates in Southwest China from 2007 to 2009

To understand the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in South China, we collected 231 clinical samples from pigs with suspected PRRSV infection in Guangdong between 2007 and 2009. We found that 74 of 231 samples were positive by RT-PCR. The PCR products of the ORF5 gene of 35 isolates from different farms were sequenced and their DNA sequences were compared to 23 other PRRSV isolates in the GenBank. We found that the nucleotide similarity among all South China isolates ranged from 87.6% to 100%, and all belonged to the North American genotype. Most of them were classified into subgenotype I, but the rest mapped to subgenotypes III, V or VI. Those in subgenotypes I and III were found to be highly variable in the primary neutralising epitope (PNE) with a specific amino acid mutation (F39/L39→I39), and a few isolates in subgenotypes I and III isolates also had a mutation at L41 (L41→S41). PRRSV isolates in subgenotypes III, V and VI had less potential glycosylation sites than those in subgenotype I. Our data contribute to the understanding of molecular variation of PRRSV in South China.

Download Full-text

Genetic Diversity Characterization of Porcine Reproductive and Respiratory Syndrome Virus Isolates in Romania, Based on Phylogenetic Analysis

International Journal of Molecular Sciences ◽

10.3390/ijms130912046 ◽

2012 ◽

Vol 13 (12) ◽

pp. 12046-12061 ◽

Cited By ~ 7

Author(s):

Mihaela Zaulet ◽

Maria Rodica Gurau ◽

Vlad Petrovan ◽

Laura Buburuzan

Keyword(s):

Genetic Diversity ◽

Phylogenetic Analysis ◽

Respiratory Syndrome Virus ◽

Virus Isolates

Download Full-text

Assessment of genetic diversity in Amomum tsao-ko Crevost & Lemarié, an important medicine food homologous crop from Southwest China using SRAP and ISSR markers

Genetic Resources and Crop Evolution ◽

10.1007/s10722-021-01204-6 ◽

2021 ◽

Author(s):

Mengli Ma ◽

Tiantao Wang ◽

Bingyue Lu

Keyword(s):

Genetic Diversity ◽

Southwest China ◽

Issr Markers

Download Full-text

Genetic diversity and population structure of rice stripe virus in China

Journal of General Virology ◽

10.1099/vir.0.006858-0 ◽

2009 ◽

Vol 90 (4) ◽

pp. 1025-1034 ◽

Cited By ~ 73

Author(s):

Tai-Yun Wei ◽

Jin-Guang Yang ◽

Fu-Long Liao ◽

Fang-Luan Gao ◽

Lian-Ming Lu ◽

...

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Yunnan Province ◽

Southwest China ◽

Eastern China ◽

Rice Stripe Virus ◽

Genetic Distances ◽

Geographical Regions ◽

Strong Negative Selection ◽

Recent Outbreak

Rice stripe virus (RSV) is one of the most economically important pathogens of rice and is repeatedly epidemic in China, Japan and Korea. The most recent outbreak of RSV in eastern China in 2000 caused significant losses and raised serious concerns. In this paper, we provide a genotyping profile of RSV field isolates and describe the population structure of RSV in China, based on the nucleotide sequences of isolates collected from different geographical regions during 1997–2004. RSV isolates could be divided into two or three subtypes, depending on which gene was analysed. The genetic distances between subtypes range from 0.050 to 0.067. The population from eastern China is composed only of subtype I/IB isolates. In contrast, the population from Yunnan province (southwest China) is composed mainly of subtype II isolates, but also contains a small proportion of subtype I/IB isolates and subtype IA isolates. However, subpopulations collected from different districts in eastern China or Yunnan province are not genetically differentiated and show frequent gene flow. RSV genes were found to be under strong negative selection. Our data suggest that the most recent outbreak of RSV in eastern China was not due to the invasion of new RSV subtype(s). The evolutionary processes contributing to the observed genetic diversity and population structure are discussed.

Download Full-text