Genetic diversity of the ORF5 gene of porcine reproductive and respiratory syndrome virus isolates in China from 2006 to 2008

To understand the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in South China, we collected 231 clinical samples from pigs with suspected PRRSV infection in Guangdong between 2007 and 2009. We found that 74 of 231 samples were positive by RT-PCR. The PCR products of the ORF5 gene of 35 isolates from different farms were sequenced and their DNA sequences were compared to 23 other PRRSV isolates in the GenBank. We found that the nucleotide similarity among all South China isolates ranged from 87.6% to 100%, and all belonged to the North American genotype. Most of them were classified into subgenotype I, but the rest mapped to subgenotypes III, V or VI. Those in subgenotypes I and III were found to be highly variable in the primary neutralising epitope (PNE) with a specific amino acid mutation (F39/L39→I39), and a few isolates in subgenotypes I and III isolates also had a mutation at L41 (L41→S41). PRRSV isolates in subgenotypes III, V and VI had less potential glycosylation sites than those in subgenotype I. Our data contribute to the understanding of molecular variation of PRRSV in South China.

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Genetic Diversity Characterization of Porcine Reproductive and Respiratory Syndrome Virus Isolates in Romania, Based on Phylogenetic Analysis

International Journal of Molecular Sciences ◽

10.3390/ijms130912046 ◽

2012 ◽

Vol 13 (12) ◽

pp. 12046-12061 ◽

Cited By ~ 7

Author(s):

Mihaela Zaulet ◽

Maria Rodica Gurau ◽

Vlad Petrovan ◽

Laura Buburuzan

Keyword(s):

Genetic Diversity ◽

Phylogenetic Analysis ◽

Respiratory Syndrome Virus ◽

Virus Isolates

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Genetic Diversity of Puumala Virus Isolates in the Republic of Tatarstan and the Republic of Mordovia

BioNanoScience ◽

10.1007/s12668-016-0331-9 ◽

2016 ◽

Vol 7 (2) ◽

pp. 309-312 ◽

Cited By ~ 2

Author(s):

Y. N. Davidyuk ◽

E. Kabwe ◽

S. F. Khaiboullina ◽

R. K. Ismagilova ◽

V. G. Shakirova ◽

...

Keyword(s):

Genetic Diversity ◽

Puumala Virus ◽

The Republic ◽

Virus Isolates

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Research Progress of Porcine Reproductive and Respiratory Syndrome

Insight - Veterinary Science ◽

10.18282/ivs.v1i1.129 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

Xueqi Liao

Keyword(s):

Genetic Diversity ◽

Genetic Variation ◽

Immune Suppression ◽

Persistent Infection ◽

The Body ◽

Research Progress ◽

Respiratory Syndrome Virus ◽

Control Technology ◽

Reproductive Disorder ◽

And Control

Porcine Reproductive and Respiratory Syndrome (PRRS) is caused by Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), which is characterized by sow reproductive disorder and piglet respiratory syndrome. The complex pathogenesis of PPRS includes antibody-dependent enhancement, immune suppression and persistent infection in the body and viral genetic diversity. In order to have a better understanding of the occurrence and prevalence of porcine reproductive and respiratory syndrome in China. The review of PRRS etiology, epidemiology, pathogenesis, genetic variation, diagnosis and control technology and other aspects of the progress were carried out.

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