scholarly journals Genetic Diversity Characterization of Porcine Reproductive and Respiratory Syndrome Virus Isolates in Romania, Based on Phylogenetic Analysis

2012 ◽  
Vol 13 (12) ◽  
pp. 12046-12061 ◽  
Author(s):  
Mihaela Zaulet ◽  
Maria Rodica Gurau ◽  
Vlad Petrovan ◽  
Laura Buburuzan
2014 ◽  
Vol 24 ◽  
pp. 193-201 ◽  
Author(s):  
Xiaomin Wang ◽  
Kongwang He ◽  
Wenwen Zhang ◽  
Zhongtao Zhou ◽  
Aihua Mao ◽  
...  

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 89
Author(s):  
Jiayu Li ◽  
Fuxian Yang ◽  
Ruobing Liang ◽  
Sheng Guo ◽  
Yaqiong Guo ◽  
...  

Cryptosporidiumfelis is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of C. felis in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (gp60) gene was employed to identify the subtypes of 30 cat-derived C. felis isolates from Guangdong and Shanghai. Altogether, 20 C. felis isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the gp60 sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of C. felis. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of C. felis.


PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33756 ◽  
Author(s):  
Gefen Yin ◽  
Libo Gao ◽  
Xianghua Shu ◽  
Guishu Yang ◽  
Shuhao Guo ◽  
...  

Viruses ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2473
Author(s):  
Zied Bouslama ◽  
Habib Kharmachi ◽  
Nourhene Basdouri ◽  
Jihen Ben Salem ◽  
Samia Ben Maiez ◽  
...  

Rabies is a viral zoonosis that is transmissible to humans via domestic and wild animals. There are two epidemiological cycles for rabies, the urban and the sylvatic cycles. In an attempt to study the epidemiological role of wild canidae in rabies transmission, the present study aimed to analyze the genetic characteristics of virus isolates and confirm prior suggestions that rabies is maintained through a dog reservoir in Tunisia. Virus strains isolated from wild canidae were subject to viral sequencing, and Bayesian phylogenetic analysis was performed using Beast2 software. Essentially, the virus strains isolated from wild canidae belonged to the Africa-1 clade, which clearly diverges from fox-related strains. Our study also demonstrated that genetic characteristics of the virus isolates were not as distinct as could be expected if a wild reservoir had already existed. On the contrary, the geographic landscape is responsible for the genetic diversity of the virus. The landscape itself could have also acted as a natural barrier to the spread of the virus.


2013 ◽  
Vol 92 (10) ◽  
pp. 2681-2686 ◽  
Author(s):  
Soo-Jeong Kye ◽  
Ji-Ye Kim ◽  
Hee-Jung Seul ◽  
Seromi Kim ◽  
Sang-Eun Kim ◽  
...  

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Artem Baidaliuk ◽  
Sébastian Lequime ◽  
Isabelle Moltini-Conclois ◽  
Stéphanie Dabo ◽  
Laura B Dickson ◽  
...  

Abstract Flaviviruses encompass not only medically relevant arthropod-borne viruses (arboviruses) but also insect-specific flaviviruses (ISFs) that are presumably maintained primarily through vertical transmission in the insect host. Interestingly, ISFs are commonly found infecting important arbovirus vectors such as the mosquito Aedes aegypti. Cell-fusing agent virus (CFAV) was the first described ISF of mosquitoes more than four decades ago. Despite evidence for widespread CFAV infections in A.aegypti populations and for CFAV potential to interfere with arbovirus transmission, little is known about CFAV evolutionary history. Here, we generated six novel CFAV genome sequences by sequencing three new virus isolates and subjecting three mosquito samples to untargeted viral metagenomics. We used these new genome sequences together with published ones to perform a global phylogenetic analysis of CFAV genetic diversity. Although there was some degree of geographical clustering among CFAV sequences, there were also notable discrepancies between geography and phylogeny. In particular, CFAV sequences from Cambodia and Thailand diverged significantly, despite confirmation that A.aegypti populations from both locations are genetically close. The apparent phylogenetic discrepancy between CFAV and its A.aegypti host in Southeast Asia indicates that other factors than host population structure shape CFAV genetic diversity.


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