Induction of Drought Tolerance in Cucumber Plants by a Consortium of Three Plant Growth-Promoting Rhizobacterium Strains

AbstractWater deficit has devastating impacts on legume production, particularly with the current abrupt climate changes in arid environments. The application of plant growth-promoting rhizobacteria (PGPR) is an effective approach for producing natural nitrogen and attenuating the detrimental effects of drought stress. This study investigated the influence of inoculation with the PGPR Rhizobium leguminosarum biovar viciae (USDA 2435) and Pseudomonas putida (RA MTCC5279) solely or in combination on the physio-biochemical and agronomic traits of five diverse Vicia faba cultivars under well-watered (100% crop evapotranspiration [ETc]), moderate drought (75% ETc), and severe drought (50% ETc) conditions in newly reclaimed poor-fertility sandy soil. Drought stress substantially reduced the expression of photosynthetic pigments and water relation parameters. In contrast, antioxidant enzyme activities and osmoprotectants were considerably increased in plants under drought stress compared with those in well-watered plants. These adverse effects of drought stress reduced crop water productivity (CWP) and seed yield‐related traits. However, the application of PGPR, particularly a consortium of both strains, improved these parameters and increased seed yield and CWP. The evaluated cultivars displayed varied tolerance to drought stress: Giza-843 and Giza-716 had the highest tolerance under well-watered and moderate drought conditions, whereas Giza-843 and Sakha-4 were more tolerant under severe drought conditions. Thus, co-inoculation of drought-tolerant cultivars with R. leguminosarum and P. putida enhanced their tolerance and increased their yield and CWP under water-deficit stress conditions. This study showed for the first time that the combined use of R. leguminosarum and P. putida is a promising and ecofriendly strategy for increasing drought tolerance in legume crops.

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Review on Drought Tolerance in Plants Induced by Plant Growth Promoting Rhizobacteria

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2018.705.053 ◽

2018 ◽

Vol 7 (05) ◽

pp. 412-422 ◽

Cited By ~ 3

Author(s):

Zaffar Mahdi Dar ◽

Amjad Masood ◽

Arshad Hussain Mughal ◽

Malik Asif ◽

Mushtaq Ahamd Malik

Keyword(s):

Drought Tolerance ◽

Plant Growth ◽

Plant Growth Promoting Rhizobacteria ◽

Plant Growth Promoting ◽

Growth Promoting

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Characterization of actinomycetes isolates for plant growth promoting traits and their effects on drought tolerance in maize

Journal of Plant Interactions ◽

10.1080/17429145.2020.1752833 ◽

2020 ◽

Vol 15 (1) ◽

pp. 93-105 ◽

Cited By ~ 4

Author(s):

Chinenyenwa Fortune Chukwuneme ◽

Olubukola Oluranti Babalola ◽

Funso Raphael Kutu ◽

Omena Bernard Ojuederie

Keyword(s):

Drought Tolerance ◽

Plant Growth ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Plant Growth Promoting Traits

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Plant growth-promoting rhizobacterium, Paenibacillus polymyxa CR1, upregulates dehydration-responsive genes, RD29A and RD29B, during priming drought tolerance in arabidopsis

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2020.08.049 ◽

2020 ◽

Vol 156 ◽

pp. 146-154

Author(s):

Wenshan Liu ◽

Edward Sikora ◽

Sang-Wook Park

Keyword(s):

Drought Tolerance ◽

Plant Growth ◽

Paenibacillus Polymyxa ◽

Plant Growth Promoting ◽

Growth Promoting

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Induction of drought tolerance in tomato upon the application of ACC deaminase producing plant growth promoting rhizobacterium Bacillus subtilis Rhizo SF 48

Microbiological Research ◽

10.1016/j.micres.2020.126422 ◽

2020 ◽

Vol 234 ◽

pp. 126422 ◽

Cited By ~ 2

Author(s):

Gowtham H.G. ◽

Brijesh Singh S. ◽

Murali M. ◽

Shilpa N. ◽

Melvin Prasad ◽

...

Keyword(s):

Bacillus Subtilis ◽

Drought Tolerance ◽

Plant Growth ◽

Acc Deaminase ◽

Plant Growth Promoting ◽

Growth Promoting

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Metabolic and physiological changes induced by plant growth regulators and plant growth promoting rhizobacteria and their impact on drought tolerance in Cicer arietinum L.

PLoS ONE ◽

10.1371/journal.pone.0213040 ◽

2019 ◽

Vol 14 (3) ◽

pp. e0213040 ◽

Cited By ~ 9

Author(s):

Naeem Khan ◽

Asghari Bano ◽

MD Ali Babar

Keyword(s):

Drought Tolerance ◽

Plant Growth ◽

Plant Growth Regulators ◽

Cicer Arietinum ◽

Growth Regulators ◽

Plant Growth Promoting Rhizobacteria ◽

Physiological Changes ◽

Cicer Arietinum L ◽

Plant Growth Promoting ◽

Growth Promoting

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Biochemical and Molecular Characterization, and Bioprospecting of Drought Tolerant Actinomycetes from Maize Rhizosphere Soil

10.1101/2020.05.13.094003 ◽

2020 ◽

Author(s):

Chinenyenwa Fortune Chukwuneme ◽

Olubukola Oluranti Babalola ◽

Funso Raphael Kutu ◽

Omena Bernard Ojuederie

Keyword(s):

Drought Tolerance ◽

Plant Growth ◽

Rna Binding ◽

Binding Protein ◽

Rrna Gene ◽

Maize Rhizosphere ◽

Siderophore Biosynthesis ◽

Drought Tolerant ◽

Plant Growth Promoting ◽

Growth Promoting

AbstractDrought is a major limitation to maize cultivation around the globe. Seven actinomycetes strains were isolated from maize rhizosphere soils in Mahikeng, North-West Province, South Africa. The isolates were biochemically characterized and identified with 16S rRNA gene sequence analysis. Isolates were also screened in vitro for abiotic stress tolerance to different concentrations of NaCl, pH, and polyethylene glycol (PEG 8000), as well as for biosynthesis of drought tolerance genes namely Glutathione peroxidase (GPX), Glycine-rich RNA binding protein (GRP), Desiccation protectant protein (DSP), Guanosine triphosphate binding protein (GTP) and plant growth-promoting genes:1-aminocyclopropane-1-carboxylate deaminase (accd) and siderophore biosynthesis (Sid). About 71.43% of isolates were of the genus Streptomyces (99-100% similarity), while 14.29% belong to the genus Arthrobacter (R15) and 14.29% to the genus Microbacterium (S11) respectively (99% similarity). Five isolates had their optimum growth at 35°C. Arthrobacter arilaitensis (R15) grew and tolerated 5%, 10%, and 20% PEG at 120 h. Root length increased by 110.53% in PEG treated maize seeds (−0.30 MPa) inoculated with Streptomyces pseudovenezuelae (S20) compared to the un-inoculated control. Likewise, germination percentage and vigor index increased by 37.53% and 194.81% respectively in PEG treated seeds inoculated with S20 than the un-inoculated PEG treated seeds. ACC deaminase gene was amplified in all the isolates, while the gene for siderophore biosynthesis was amplified in 85.71% of the isolates. Genes for the synthesis of GPX, GRP, DSP and GTP were amplified in Arthrobacter arilaitensis (R15) and Streptomyces pseudovenezuelae (S20) which lacked GTP. The amplification of drought-tolerant and plant growth-promoting primers indicates the possible presence of these genes in the isolates. These isolates have the potential for use as bio-inoculants, not only to improve drought tolerance in maize but also to be utilized as biofertilizers and biocontrol agents to facilitate growth promotion.

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